scholarly journals Designing fluorescent biosensors using circular permutations of riboswitches

Methods ◽  
2018 ◽  
Vol 143 ◽  
pp. 102-109 ◽  
Author(s):  
Johnny Truong ◽  
Yu-Fang Hsieh ◽  
Lynda Truong ◽  
Guifang Jia ◽  
Ming C. Hammond
2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.


Author(s):  
A.A. Lugovski ◽  
M.P. Samtsov ◽  
A.P. Lugovski ◽  
E.S. Voropay ◽  
A.V. Lavysh ◽  
...  

2007 ◽  
Vol 51 (1) ◽  
pp. 95-96
Author(s):  
H. Hayashi ◽  
M. Inoue ◽  
T. Morii

Author(s):  
Andrew Solomon ◽  
Paul Sutcliffe ◽  
Raymond Lister

2020 ◽  
Vol 11 (9) ◽  
pp. 5258 ◽  
Author(s):  
Danil S. Serdyukov ◽  
Tatiana N. Goryachkovskaya ◽  
Irina A. Mescheryakova ◽  
Svetlana V. Bannikova ◽  
Sergei A. Kuznetsov ◽  
...  

2013 ◽  
Vol 104 (2) ◽  
pp. 529a ◽  
Author(s):  
Claudia Solscheid ◽  
Martin R. Webb

2019 ◽  
Vol 12 (1) ◽  
pp. 157-169
Author(s):  
Daniel Gray ◽  
Charles Lanning ◽  
Hua Wang

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