scholarly journals Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors

2020 ◽  
Vol 11 (9) ◽  
pp. 5258 ◽  
Author(s):  
Danil S. Serdyukov ◽  
Tatiana N. Goryachkovskaya ◽  
Irina A. Mescheryakova ◽  
Svetlana V. Bannikova ◽  
Sergei A. Kuznetsov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Terahertz Radiation ◽  
Gene Networks ◽  
Fluorescent Biosensors

scholarly journals Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli

iScience ◽  
2019 ◽  
Vol 14 ◽  
pp. 323-334 ◽  
Author(s):  
Alex J.H. Fedorec ◽  
Tanel Ozdemir ◽  
Anjali Doshi ◽  
Yan-Kay Ho ◽  
Luca Rosa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks

scholarly journals Automatic detection of changes in the dynamics of delayed stochastic gene networks and in vivo production of RNA molecules in Escherichia coli

2011 ◽  
Vol 27 (19) ◽  
pp. 2714-2720 ◽  
Author(s):  
Jarno Mäkelä ◽  
Heikki Huttunen ◽  
Meenakshisundaram Kandhavelu ◽  
Olli Yli-Harja ◽  
Andre S. Ribeiro
Keyword(s):  
Escherichia Coli ◽  
Gene Networks ◽  
Automatic Detection ◽  
Rna Molecules ◽  
Stochastic Gene Networks

scholarly journals Pathogenic potential in catheter associated Escherichia coli is associated with separable biofilm and virulence gene determinants

2021 ◽  
Author(s):  
Zongsen Zou ◽  
Robert Potter ◽  
William McCoy ◽  
George Katumba ◽  
Peter J. Mucha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Biofilm Formation ◽  
Gene Networks ◽  
Bacterial Colonization ◽  
Asymptomatic Bacteriuria ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Pathogenic Potential ◽  
E Coli

Urinary catheterization facilitates asymptomatic bacterial colonization of the urinary tract and increases the risk of urinary tract infection (UTI). Differentiating catheter-associated asymptomatic bacteriuria (CAASB) from catheter-associated UTI (CAUTI) can be challenging when the presence or origins of symptoms are unclear. To determine whether strain-specific Escherichia coli characteristics are associated with CAUTI, we compared genomic composition and experimental catheter biofilm production by urinary isolates from catheterized inpatients and rectal isolates from healthy volunteers. CAUTI isolates were associated with a distinctive phylotype B2 sub-clade dominated by the multidrug resistant, pandemic ST131 lineage. While catheter biofilm formation was widespread among E. coli isolates, phylotype B2 biofilms were more extensive and biofilm-associated genes were preferentially found in B2 and ST131 isolates. Distinctive suites of iron-responsive genes were associated with both the ST131 lineage and catheter biofilm formation. Catheter biofilms produced by some CAASB strains could inhibit ST131 colonization, suggesting a potential beneficial function for these strains. These results suggest that the combination of biofilm and non-biofilm-associated gene networks in urinary E. coli influence CAUTI risk in catheterized patients.

Stress-induced systems in Escherichia coli and their response to terahertz radiation

2017 ◽  
Vol 7 (8) ◽  
pp. 858-868 ◽  
Author(s):  
S. E. Peltek ◽  
E. V. Demidova ◽  
V. M. Popik ◽  
T. N. Goryachkovskaya
Keyword(s):  
Escherichia Coli ◽  
Terahertz Radiation

scholarly journals Escherichia coli stress response systems and their reaction to terahertz radiation

2016 ◽  
Vol 20 (6) ◽  
pp. 876-886
Author(s):  
S. E. Peltek ◽  
E. V. Demidova ◽  
V. M. Popik ◽  
T. N. Goryachkovskaya
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Terahertz Radiation ◽  
Response Systems

scholarly journals Engineered orthogonal quorum sensing systems for synthetic gene regulation

2018 ◽  
Author(s):  
Stefan J. Tekel ◽  
Christina L. Smith ◽  
Brianna Lopez ◽  
Amber Mani ◽  
Christopher Connot ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Signaling Pathways ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks ◽  
Homoserine Lactones ◽  
Signaling Systems ◽  
Sensing Systems ◽  
Engineered Bacteria

AbstractGene regulators that are controlled by membrane-permeable compounds called Homoserine lactones (HSLs) have become popular tools for building synthetic gene networks that coordinate behaviors across populations of engineered bacteria. Synthetic HSL-signaling systems are derived from natural DNA and protein elements from microbial quorum signaling pathways. Crosstalk, where a single HSL can activate multiple regulators, can lead to faults in networks composed of parallel signaling pathways. Here, we report an investigation of quorum sensing components to identify synthetic pathways that exhibit little to no crosstalk in liquid and solid cultures. In previous work, we characterized the response of a single regulator (LuxR) to ten distinct HSL-synthase enzymes. Our current study determined the responses of five different regulators (LuxR, LasR, TraR, BjaR, and AubR) to the same set of synthases. We identified two sets of orthogonal synthase-regulator pairs (BjaI/BjaR + EsaI/TraR and LasI/LasR + EsaI/TraR) that show little to no crosstalk when they are expressed in Escherichia coli BL21. These results expand the toolbox of characterized components for engineering microbial communities.

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

The Nature of the Intramembrane Particle

1980 ◽  
Vol 38 ◽  
pp. 688-691
Author(s):  
A.J. Verkleij
Keyword(s):  
Escherichia Coli ◽  
Tight Junction ◽  
Gap Junction ◽  
The Other ◽  
Fracture Face ◽  
Band 3 Protein ◽  
Satisfactory Explanation ◽  
Freeze Fracturing ◽  
Intramembrane Particle ◽  
Luminal Membrane

Freeze-fracturing splits membranes into two helves, thus allowing an examination of the membrane interior. The 5-10 rm particles visible on both monolayers are widely assumed to be proteinaceous in nature. Most membranes do not reveal impressions complementary to particles on the opposite fracture face, if the membranes are fractured under conditions without etching. Even if it is considered that shadowing, contamination or fracturing itself might obscure complementary pits', there is no satisfactory explanation why under similar physical circimstances matching halves of other membranes can be visualized. A prominent example of uncomplementarity is found in the erythrocyte manbrane. It is wall established that band 3 protein and possibly glycophorin represents these nonccmplanentary particles. On the other hand a number of membrane types show pits opposite the particles. Scme well known examples are the ";gap junction',"; tight junction, the luminal membrane of the bladder epithelial cells and the outer membrane of Escherichia coli.

Sign in / Sign up

Export Citation Format

Share Document