Corrigendum to ‘Visualizing viral protein structures in cells using genetic probes for correlated light and electron microscopy’ [Methods 90 (2015) 39–48]

Methods ◽  
2016 ◽  
Vol 104 ◽  
pp. 206
Author(s):  
Horng D. Ou ◽  
Thomas J. Deerinck ◽  
Eric Bushong ◽  
Mark H. Ellisman ◽  
Clodagh C. O’Shea
Keyword(s):  
Electron Microscopy ◽  
Viral Protein ◽  
Protein Structures ◽  
Light And Electron Microscopy ◽  
Genetic Probes ◽  
In Cells

scholarly journals Visualizing viral protein structures in cells using genetic probes for correlated light and electron microscopy

Methods ◽  
2015 ◽  
Vol 90 ◽  
pp. 39-48 ◽  
Author(s):  
Horng D. Ou ◽  
Thomas J. Deerinck ◽  
Eric Bushong ◽  
Mark H. Ellisman ◽  
Clodagh C. O’Shea
Keyword(s):  
Electron Microscopy ◽  
Viral Protein ◽  
Protein Structures ◽  
Light And Electron Microscopy ◽  
Genetic Probes ◽  
In Cells

scholarly journals Electron Microscopy of the Bovine Cutaneous Papilloma

1966 ◽  
Vol 3 (3) ◽  
pp. 196-207 ◽  
Author(s):  
D. Brobst ◽  
E.J. Hinsman
Keyword(s):  
Electron Microscopy ◽  
Plasma Membranes ◽  
Light And Electron Microscopy ◽  
Degenerative Changes ◽  
Virus Like Particles ◽  
Cytoplasmic Vacuolation ◽  
Naturally Occurring ◽  
Prickle Cell ◽  
In Cells

Experimentally produced bovine cutaneous papillomas of 30, 76 and 112 days' duration as well as 3 naturally occurring bovine cutaneous papillomas were examined by light and electron microscopy. Degenerative changes were not observed in the fibromatous base of the 30-day-old papilloma. An alteration in all papillomas older than 30 days was cytoplasmic vacuolation in cells of the prickle cell and keratohyaline layers. As cells progressed upward their degeneration became more prominent. In the keratinized layers of 2 papillomas virus-like particles were observed within plasma membranes of keratinized cells.

Light and electron microscopy localization of chloride ions in cells of Salicornia pacifica var. utahensis

1975 ◽  
Vol 53 (12) ◽  
pp. 1176-1187 ◽  
Author(s):  
W. M. Hess ◽  
D. J. Hansen ◽  
D. J. Weber
Keyword(s):  
Electron Microscopy ◽  
Vascular System ◽  
Silver Chloride ◽  
Light And Electron Microscopy ◽  
Cell Types ◽  
Chloride Ions ◽  
Vascular Bundles ◽  
Silver Acetate ◽  
Parenchyma Cells ◽  
In Cells

Light and electron microscopy was used to determine the distribution of chloride ions in cells and tissues of Salicornia pacifica Standl, var. utahensis (Tidestrom) Munz. Chlorenchyma cells with chloroplasts around the periphery and sclereid-like cells with distinct wall thickenings which extended from the anastomosing vascular system to near the epidermis were present in the cortex. The vascular bundles or stelar strands were surrounded by several layers of large parenchyma cells. Tissues were treated with silver acetate for silver chloride precipitation. Silver chloride precipitation sites were present in all cell types. Precipitation sites were readily evident in the vacuoles but not in other organelles.

Tubulin Structure and the Development of Electron Crystallography

1999 ◽  
Vol 5 (S2) ◽  
pp. 406-407
Author(s):  
Kenneth H. Downing
Keyword(s):  
Electron Microscopy ◽  
Dynamic Properties ◽  
Protein Structures ◽  
Light And Electron Microscopy ◽  
Major Protein ◽  
Electron Crystallography ◽  
Technical Problems ◽  
3 Dimensional ◽  
Technical Developments ◽  
Tubulin Structure

The identification of tubulin as the major protein component of microtubules (1) occurred at about the same time, a little over thirty years ago, as the first demonstration of the ability to obtain 3- dimensional data from electron micrographs. DeRosier and Klug's paper on image reconstruction of the bacteriophage tail (2) marks what many consider the beginning of electron crystallography - determining protein structures by electron microscopy. Microtubules have long been the target of active study by light and electron microscopy, and even before the discovery of tubulin played a significant role in driving technical developments in EM. The demonstration that tubulin could form two-dimensional crystalline sheets (3) seemed to pave the way for high resolution .structural studies. The discovery of the sheet polymer of tubulin came shortly after Unwin and Henderson's first publications on bacteriorhodopsin showing secondary structure resolved by EM (4). Application of their methods to tubulin in the late 1970's resolved low resolution features of the protein, but technical problems prevented achieving much more than showing the molecular envelope. Many developments in both tubulin studies and electron crystallography followed this work over the next 15 years. As techniques were refined for purifying, labeling and visualizing tubulin and microtubules, the dynamic properties of microtubules and their role in the cell cycle were characterized, and some hints about the mechanism of polymerization and disassembly were derived from biophysical and microscopic investigations.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

Ultrastructure of two neoplasms in culture compared with original biopsies

1984 ◽  
Vol 42 ◽  
pp. 50-51
Author(s):  
Joseph M. Harb ◽  
James T. Casper ◽  
Vlcki Piaskowski
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Biopsy Specimen ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells ◽  
Morphological Distinction ◽  
Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

The Effect of Vitamin A on the Intermittent Nitrogen Dioxide Gas Exposure in Hamsters

1976 ◽  
Vol 34 ◽  
pp. 176-177
Author(s):  
J.C.S. Kim ◽  
M.G. Jourden ◽  
E.S. Carlisle
Keyword(s):  
Electron Microscopy ◽  
Vitamin A ◽  
Nitrogen Dioxide ◽  
Chronic Exposure ◽  
Light And Electron Microscopy ◽  
Specific Effect ◽  
Deficient Diet ◽  
Intermittent Exposure ◽  
Hypovitaminosis A ◽  
Gas Exposure

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

The Response of Testis Cells to Low Dose X-Irradiation

1981 ◽  
Vol 39 ◽  
pp. 542-543
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
Joann Stevenson ◽  
S. Black
Keyword(s):  
Electron Microscopy ◽  
Stem Cell ◽  
Light Microscopy ◽  
Dose Level ◽  
Cross Sections ◽  
Low Dose ◽  
Light And Electron Microscopy ◽  
Cellular Responses ◽  
Post Irradiation ◽  
X Irradiation

The response of spermatogonial cells to X-irradiation is well documented. It has been shown that there is a radiation resistent stem cell (As) which, after irradiation, replenishes the seminiferous epithelium. Most investigations in this area have dealt with radiation dosages of 100R or more. This study was undertaken to observe cellular responses at doses less than 100R of X-irradiation utilizing a system in which the tissue can be used for light and electron microscopy.Brown B6D2F1 mice aged 16 weeks were exposed to X-irradiation (225KeV; 15mA; filter 0.35 Cu; 50-60 R/min). Four mice were irradiated at each dose level between 1 and 100 rads. Testes were removed 3 days post-irradiation, fixed, and embedded. Sections were cut at 2 microns for light microscopy. After staining, surviving spermatogonia were identified and counted in tubule cross sections. The surviving fraction of spermatogonia compared to control, S/S0, was plotted against dose to give the curve shown in Fig. 1.

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