scholarly journals In vivo imaging of C. elegans endocytosis

Methods ◽  
2014 ◽  
Vol 68 (3) ◽  
pp. 518-528 ◽  
Author(s):  
Lei Wang ◽  
Anjon Audhya
Keyword(s):  
In Vivo Imaging ◽  
C Elegans

Airy light sheet microscope for in-vivo imaging of C. elegans

2021 ◽  
Author(s):  
Hung-Chuan Hsu ◽  
Sunil Vyas ◽  
Kuang-Yuh Huang ◽  
Hsien-Shun Liao ◽  
Yuan Luo
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
C Elegans

A near-infrared chemodosimeter with Pi-selective colorimetric and fluorescent sensing and its application in vivo imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (88) ◽  
pp. 71756-71759 ◽  
Author(s):  
Tie Nan Zang ◽  
Rui Rui Zhao ◽  
Xing Zhu Yang ◽  
Yan Gao ◽  
Guang Ke Wang ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Chemosensor ◽  
Practical Utility ◽  
Fluorescent Sensing ◽  
C Elegans ◽  
Phosphate Ion

A near-infrared colorimetric and fluorescent chemosensor for detecting phosphate ion (Pi) has been developed. The practical utility of this chemosensor was demonstrated by employing it to detect Pi in Paramecium and C. elegans.

scholarly journals In Vivo Imaging of C. elegans Mechanosensory Neurons Demonstrates a Specific Role for the MEC-4 Channel in the Process of Gentle Touch Sensation

Neuron ◽  
2003 ◽  
Vol 39 (6) ◽  
pp. 1005-1017 ◽  
Author(s):  
Hiroshi Suzuki ◽  
Rex Kerr ◽  
Laura Bianchi ◽  
Christian Frøkjær-Jensen ◽  
Dan Slone ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Specific Role ◽  
C Elegans ◽  
Mechanosensory Neurons ◽  
Touch Sensation

scholarly journals In vivo imaging of retrogradely transported synaptic vesicle proteins in C. elegans neurons

2010 ◽  
Vol 344 (1) ◽  
pp. 508-509
Author(s):  
Kausalya T. Murthy ◽  
Jaffar Bhat ◽  
Sandhya P. Koushika
Keyword(s):  
Synaptic Vesicle ◽  
In Vivo Imaging ◽  
C Elegans ◽  
Vesicle Proteins

scholarly journals In vivo imaging of C. elegans ASH neurons: cellular response and adaptation to chemical repellents

2004 ◽  
Vol 24 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Massimo A Hilliard ◽  
Alfonso J Apicella ◽  
Rex Kerr ◽  
Hiroshi Suzuki ◽  
Paolo Bazzicalupo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Cellular Response ◽  
C Elegans ◽  
Chemical Repellents

A naphthalimide-based fluorescent probe for highly selective detection of histidine in aqueous solution and its application in in vivo imaging

2015 ◽  
Vol 51 (15) ◽  
pp. 3143-3146 ◽  
Author(s):  
Hio-Ieng Un ◽  
Shuai Wu ◽  
Chang-Bo Huang ◽  
Zheng Xu ◽  
Lin Xu
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Living Cells ◽  
Selective Detection ◽  
C Elegans

A naphthalimide-based fluorescent probe for selectively detecting His in aqueous solution, living cells, andC. eleganshas been developed.

scholarly journals E-Cadherin/HMR-1 Membrane Enrichment Is Polarized by WAVE-Dependent Branched Actin

2021 ◽  
Vol 9 (2) ◽  
pp. 19
Author(s):  
Luigy Cordova-Burgos ◽  
Falshruti B. Patel ◽  
Martha C. Soto
Keyword(s):  
Cell Polarity ◽  
In Vivo Imaging ◽  
C Elegans ◽  
Lateral Membrane ◽  
Junction Protein ◽  
Apical Junctions ◽  
Membrane Changes ◽  
Apical Membranes ◽  
E Cadherin

Polarized epithelial cells adhere to each other at apical junctions that connect to the apical F-actin belt. Regulated remodeling of apical junctions supports morphogenesis, while dysregulated remodeling promotes diseases such as cancer. We have documented that branched actin regulator, WAVE, and apical junction protein, Cadherin, assemble together in developing C. elegans embryonic junctions. If WAVE is missing in embryonic epithelia, too much Cadherin assembles at apical membranes, and yet apical F-actin is reduced, suggesting the excess Cadherin is not fully functional. We proposed that WAVE supports apical junctions by regulating the dynamic accumulation of Cadherin at membranes. To test this model, here we examine if WAVE is required for Cadherin membrane enrichment and apical–basal polarity in a maturing epithelium, the post-embryonic C. elegans intestine. We find that larval and adult intestines have distinct apicobasal populations of Cadherin, each with distinct dependence on WAVE branched actin. In vivo imaging shows that loss of WAVE components alters post-embryonic E-cadherin membrane enrichment, especially at apicolateral regions, and alters the lateral membrane. Analysis of a biosensor for PI(4,5)P2 suggests loss of WAVE or Cadherin alters the polarity of the epithelial membrane. EM (electron microscopy) illustrates lateral membrane changes including separations. These findings have implications for understanding how mutations in WAVE and Cadherin may alter cell polarity.

scholarly journals Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging

2020 ◽  
Author(s):  
Yong Qian ◽  
Danielle M. Orozco Cosio ◽  
Kiryl D. Piatkevich ◽  
Sarah Aufmkolk ◽  
Wan-Chi Su ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Calcium Ion ◽  
Near Infrared ◽  
Cultured Cells ◽  
State Of The Art ◽  
Brain Slices ◽  
C Elegans ◽  
Visible Wavelength ◽  
Mammalian Tissues

AbstractNear-infrared (NIR) genetically-encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross-talk with visible-light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report two improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and C. elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.

Sign in / Sign up

Export Citation Format

Share Document