A near-infrared chemodosimeter with Pi-selective colorimetric and fluorescent sensing and its application in vivo imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (88) ◽  
pp. 71756-71759 ◽  
Author(s):  
Tie Nan Zang ◽  
Rui Rui Zhao ◽  
Xing Zhu Yang ◽  
Yan Gao ◽  
Guang Ke Wang ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Chemosensor ◽  
Practical Utility ◽  
Fluorescent Sensing ◽  
C Elegans ◽  
Phosphate Ion

A near-infrared colorimetric and fluorescent chemosensor for detecting phosphate ion (Pi) has been developed. The practical utility of this chemosensor was demonstrated by employing it to detect Pi in Paramecium and C. elegans.

scholarly journals Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging

2020 ◽  
Author(s):  
Yong Qian ◽  
Danielle M. Orozco Cosio ◽  
Kiryl D. Piatkevich ◽  
Sarah Aufmkolk ◽  
Wan-Chi Su ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Calcium Ion ◽  
Near Infrared ◽  
Cultured Cells ◽  
State Of The Art ◽  
Brain Slices ◽  
C Elegans ◽  
Visible Wavelength ◽  
Mammalian Tissues

AbstractNear-infrared (NIR) genetically-encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross-talk with visible-light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report two improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and C. elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

scholarly journals Molecular imaging and deep learning analysis of uMUC1 expression in response to chemotherapy in an orthotopic model of ovarian cancer

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hongwei Zhao ◽  
Hasaan Hayat ◽  
Xiaohong Ma ◽  
Daguang Fan ◽  
Ping Wang ◽  
...  
Keyword(s):  
Neural Networks ◽  
Ovarian Cancer ◽  
Deep Learning ◽  
Cancer Progression ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Response To Therapy ◽  
Response To Chemotherapy

Abstract Artificial Intelligence (AI) algorithms including deep learning have recently demonstrated remarkable progress in image-recognition tasks. Here, we utilized AI for monitoring the expression of underglycosylated mucin 1 (uMUC1) tumor antigen, a biomarker for ovarian cancer progression and response to therapy, using contrast-enhanced in vivo imaging. This was done using a dual-modal (magnetic resonance and near infrared optical imaging) uMUC1-specific probe (termed MN-EPPT) consisted of iron-oxide magnetic nanoparticles (MN) conjugated to a uMUC1-specific peptide (EPPT) and labeled with a near-infrared fluorescent dye, Cy5.5. In vitro studies performed in uMUC1-expressing human ovarian cancer cell line SKOV3/Luc and control uMUC1low ES-2 cells showed preferential uptake on the probe by the high expressor (n = 3, p < .05). A decrease in MN-EPPT uptake by SKOV3/Luc cells in vitro due to uMUC1 downregulation after docetaxel therapy was paralleled by in vivo imaging studies that showed a reduction in probe accumulation in the docetaxel treated group (n = 5, p < .05). The imaging data were analyzed using deep learning-enabled segmentation and quantification of the tumor region of interest (ROI) from raw input MRI sequences by applying AI algorithms including a blend of Convolutional Neural Networks (CNN) and Fully Connected Neural Networks. We believe that the algorithms used in this study have the potential to improve studying and monitoring cancer progression, amongst other diseases.

scholarly journals Extending the Near-Infrared Emission Range of Indium Phosphide Quantum Dots for Multiplexed In Vivo Imaging

Nano Letters ◽  
2021 ◽  
Author(s):  
Alexander M. Saeboe ◽  
Alexey Yu. Nikiforov ◽  
Reyhaneh Toufanian ◽  
Joshua C. Kays ◽  
Margaret Chern ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Indium Phosphide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Infrared Emission ◽  
Near Infrared Emission

A macrophage uptaking near-infrared chemical probe CDnir7 for in vivo imaging of inflammation

2014 ◽  
Vol 50 (50) ◽  
pp. 6589 ◽  
Author(s):  
Nam-Young Kang ◽  
Sung-Jin Park ◽  
Xiao Wei Emmiline Ang ◽  
Animesh Samanta ◽  
Wouter H. P. Driessen ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Chemical Probe

A hypoxia-activated near infrared fluorescent probe for cyclooxygenase-2 and in vivo imaging for tumor and inflammation

2018 ◽  
Vol 265 ◽  
pp. 582-590 ◽  
Author(s):  
Yuezheng Ti ◽  
Ling Yu ◽  
Yao Tang ◽  
Tongxia Jin ◽  
Ming Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Cyclooxygenase 2
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