The intracellular antibody capture technology: towards the high-throughput selection of functional intracellular antibodies for target validation

Methods ◽  
2004 ◽  
Vol 34 (2) ◽  
pp. 200-214 ◽  
Author(s):  
M VISINTIN
Keyword(s):  
High Throughput ◽  
Target Validation ◽  
Intracellular Antibody ◽  
Antibody Capture ◽  
Selection Of

Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

2002 ◽  
Vol 317 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Eric Tse ◽  
M.Natividad Lobato ◽  
Alan Forster ◽  
Tomoyuki Tanaka ◽  
Grace T.Y Chung ◽  
...  
Keyword(s):  
Technology Application ◽  
Intracellular Antibody ◽  
Antibody Capture ◽  
Oncogenic Protein ◽  
Selection Of

High-throughput target validation in model organisms

2004 ◽  
Vol 3 (5) ◽  
pp. 191-197 ◽  
Author(s):  
Thanh N. Doan ◽  
Carmen D. Eilertson ◽  
Amy L. Rubinstein
Keyword(s):  
High Throughput ◽  
Model Organisms ◽  
Target Validation

scholarly journals A novel approach for rapid high-throughput selection of recombinant functional rat monoclonal antibodies

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Qin Chen ◽  
Shengping Qiu ◽  
Huanhuan Li ◽  
Chaolong Lin ◽  
Yong Luo ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
Novel Approach ◽  
Selection Of

Prediction of Stilbene Content from Heartwood Increment Cores of Scots Pine Using near Infrared Spectroscopy Methodology

2016 ◽  
Vol 24 (6) ◽  
pp. 517-528 ◽  
Author(s):  
Susanna Pulkka ◽  
Vincent Segura ◽  
Anni Harju ◽  
Tarja Tapanila ◽  
Johanna Tanner ◽  
...  
Keyword(s):  
Scots Pine ◽  
High Throughput ◽  
Near Infrared ◽  
Nir Spectroscopy ◽  
Tree Breeding ◽  
Decay Resistance ◽  
Increment Core ◽  
Automatic Selection ◽  
Non Destructive ◽  
Selection Of

High-throughput and non-destructive methods for quantifying the content of the stilbene compounds of Scots pine ( Pinus sylvestris L.) heartwood are needed in the breeding for decay resistance of heartwood timber. In this study, near infrared (NIR) spectroscopy calibrations were developed for a large collection of solid heartwood increment core samples in order to predict the amount of the stilbene pinosylvin (PS), its monomethyl ether (PSM) and their sum (STB). The resulting models presented quite accurate predictions in an independent validation set with R2V values ranging between 0.79 and 0.91. The accuracy of the models strongly depended on the chemical being calibrated, with the lowest accuracy for PS, intermediate accuracy for PSM and highest accuracy for STB. The effect of collecting one, two or more (up to five) spectra per sample on the calibration models was studied and it was found that averaging multiple spectra yielded better accuracy as it may account for the heterogeneity of wood along the increment core within and between rings. Several statistical pretreatments of the spectra were tested and an automatic selection of wavenumbers prior to calibration. Without the automatic selection of wavenumbers, a first derivative of normalised spectra yielded the best accuracies, whereas after the automatic selection of wavenumbers, no particular statistical pretreatment appeared to yield better results than any other. Finally, the automatic selection of wavenumbers slightly improved the accuracy of the models for all traits. These results demonstrate the potential of NIR spectroscopy as a high-throughput and non-destructive phenotyping technique in tree breeding for the improvement of decay resistance in heartwood timber.

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