scholarly journals Yolk proteolysis and aquaporin-1o play essential roles to regulate fish oocyte hydration during meiosis resumption

2006 ◽  
Vol 295 (1) ◽  
pp. 250-262 ◽  
Author(s):  
Mercedes Fabra ◽  
Demetrio Raldúa ◽  
María G. Bozzo ◽  
Peter M.T. Deen ◽  
Esther Lubzens ◽  
...  
Keyword(s):  
Oocyte Hydration ◽  
Fish Oocyte

Physiological and molecular basis of fish oocyte hydration

2007 ◽  
pp. 349-396 ◽  
Author(s):  
Joan Cerdà ◽  
Mercedes Fabra ◽  
Demetrio Raldúa
Keyword(s):  
Molecular Basis ◽  
Oocyte Hydration ◽  
Fish Oocyte

Fish Oocyte Ageing and its Effect on Egg Quality

2015 ◽  
Vol 23 (3) ◽  
pp. 302-314 ◽  
Author(s):  
Azin Mohagheghi Samarin ◽  
Tomas Policar ◽  
Franz Lahnsteiner
Keyword(s):  
Egg Quality ◽  
Fish Oocyte

scholarly journals Effect of a low water concentration in chloride, sodium and potassium on oocyte maturation, oocyte hydration, ovulation and egg quality in rainbow trout

Aquaculture ◽  
2022 ◽  
Vol 546 ◽  
pp. 737374
Author(s):  
Emilien Segret ◽  
Emilie Cardona ◽  
Sandrine Skiba-Cassy ◽  
Frédéric Cachelou ◽  
Julien Bobe
Keyword(s):  
Rainbow Trout ◽  
Oocyte Maturation ◽  
Egg Quality ◽  
Water Concentration ◽  
Oocyte Hydration ◽  
Sodium And Potassium

In vivo oocyte hydration in Atlantic halibut (Hippoglossus hippoglossus); proteolytic liberation of free amino acids, and ion transport, are driving forces for osmotic water influx

2002 ◽  
Vol 205 (2) ◽  
pp. 211-224
Author(s):  
Roderick Nigel Finn ◽  
Gunn C. Østby ◽  
Birgitta Norberg ◽  
Hans Jørgen Fyhn
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Driving Forces ◽  
Atlantic Halibut ◽  
Hippoglossus Hippoglossus ◽  
Oocyte Hydration ◽  
Osmotic Water ◽  
Cellular Water

SUMMARY The in vivo swelling and hydration of maturing oocytes of Atlantic halibut Hippoglossus hippoglossus were studied in order to characterise the osmotic mechanism underlying oocyte hydration in oviparous marine teleosts that spawn pelagic eggs. Sequential biopsies from two females, spanning four hydration cycles, were examined by osmometry, solute analysis and electrophoresis of dissected hydrating oocytes and ovulated eggs. The hydration cycle of the biopsied halibuts lasted 33–54 h. The majority of ovarian oocytes existed in a pre-hydrated condition (individual wet mass approx. 3.7 mg, diameter approx. 1.87 mm, 63 % H2O) with easily visible, non-coalesced, yolk platelets. Group-synchronous batches of the pre-hydrated oocytes increased in individual wet mass, diameter and water content to reach the ovulated egg stage of approximately 15 mg, 3.0 mm and 90 % H2O, respectively. The yolk osmolality of the hydrating oocytes was transiently hyperosmotic to the ovarian fluid (range 305–350 mOsmol l–1) with a peak osmolality of about 450 mOsmol l–1 in oocytes of 6–8 mg individual wet mass. The transient hyperosmolality was well accounted for by the increase in oocyte content of free amino acids (FAAs; approx. 2300 nmol oocyte–1), K+ (approx. 750 nmol oocyte–1), Cl– (approx. 900 nmol oocyte–1), total ammonium (approx. 300 nmol oocyte–1) and inorganic phosphate (Pi; approx. 200 nmol oocyte–1) when relating to the increase in cellular water. The oocyte content of Na+ did not increase during the hydration phase. Extensive proteolysis of yolk proteins, in particular a 110 kDa protein, correlated with the increase in the FAA pool, although the latter increased by approx. 20 % more than could be accounted for by the decrease in the oocyte protein content. Both indispensable and dispensable amino acids increased in the FAA pool, and particularly serine, alanine, leucine, lysine, glutamine and glutamate. Taurine content remained stable at approx. 70 nmol oocyte–1 during oocyte hydration. The results show that final hydration of Atlantic halibut oocytes is caused by an osmotic water uptake in which FAAs, derived mainly from the hydrolysis of a 110 kDa yolk protein, contribute approximately 50 % of the yolk osmolality and ions (Cl–, K+, Pi, NH4+) make up the balance.

scholarly journals Study differentiating fish oocyte developmental stages using bioimpedance spectroscopy

Aquaculture ◽  
2021 ◽  
pp. 737396
Author(s):  
Eloïse Detrez ◽  
Vincent Kerzérho ◽  
Mohamed-Moez Belhaj ◽  
Alain Vergnet ◽  
Hugues de Verdal ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Bioimpedance Spectroscopy ◽  
Fish Oocyte
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