scholarly journals Characterization of the CD8+ T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice

Virology ◽  
2006 ◽  
Vol 352 (1) ◽  
pp. 157-168 ◽  
Author(s):  
Michaël V. Lukens ◽  
Erwin A.W. Claassen ◽  
Patricia M.A. de Graaff ◽  
Mariska E.A. van Dijk ◽  
Peter Hoogerhout ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
T Cell ◽  
Secondary Infection ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Cell Responses ◽  
Syncytial Virus

scholarly journals Functional characterization of CD4 and CD8 T cell responses among human papillomavirus infected patients with ano-genital warts

VirusDisease ◽  
2017 ◽  
Vol 28 (2) ◽  
pp. 133-140 ◽  
Author(s):  
Manjula Singh ◽  
Deepshi Thakral ◽  
Narayan Rishi ◽  
Hemanta Kumar Kar ◽  
Dipendra Kumar Mitra
Keyword(s):  
Human Papillomavirus ◽  
T Cell ◽  
Functional Characterization ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Genital Warts ◽  
Cell Responses

scholarly journals Gene-gun DNA vaccination aggravates respiratory syncytial virus-induced pneumonitis

2004 ◽  
Vol 85 (10) ◽  
pp. 3017-3026 ◽  
Author(s):  
Christina Bartholdy ◽  
Wieslawa Olszewska ◽  
Anette Stryhn ◽  
Allan Randrup Thomsen ◽  
Peter J. M. Openshaw
Keyword(s):  
Weight Loss ◽  
Respiratory Syncytial Virus ◽  
T Cell ◽  
T Cell Responses ◽  
Dna Vaccination ◽  
T Cell Memory ◽  
Gene Gun ◽  
Cell Responses ◽  
Virus Clearance ◽  
Syncytial Virus

A CD8+ T-cell memory response to respiratory syncytial virus (RSV) was generated by using a DNA vaccine construct encoding the dominant Kd-restricted epitope from the viral transcription anti-terminator protein M2 (M282–90), linked covalently to human β 2-microglobulin (β 2m). Cutaneous gene-gun immunization of BALB/c mice with this construct induced an antigen-specific CD8+ T-cell memory. After intranasal RSV challenge, accelerated CD8+ T-cell responses were observed in pulmonary lymph nodes and virus clearance from the lungs was enhanced. The construct induced weaker CD8+ T-cell responses than those elicited with recombinant vaccinia virus expressing the complete RSV M2 protein, but stronger than those induced by a similar DNA construct without the β 2m gene. DNA vaccination led to enhanced pulmonary disease after RSV challenge, with increased weight loss and cell recruitment to the lung. Depletion of CD8+ T cells reduced, but did not abolish, enhancement of disease. Mice vaccinated with a construct encoding a class I-restricted lymphocytic choriomeningitis virus epitope and β 2m suffered more severe weight loss after RSV infection than unvaccinated RSV-infected mice, although RSV-specific CD8+ T-cell responses were not induced. Thus, in addition to specific CD8+ T cell-mediated immunopathology, gene-gun DNA vaccination causes non-specific enhancement of RSV disease without affecting virus clearance.

scholarly journals Regulatory CD4+CD25+ T Cells Restrict Memory CD8+ T Cell Responses

2002 ◽  
Vol 196 (12) ◽  
pp. 1585-1592 ◽  
Author(s):  
Mischo Kursar ◽  
Kerstin Bonhagen ◽  
Joachim Fensterle ◽  
Anne Köhler ◽  
Robert Hurwitz ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Secondary Infection ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
T Cell Response ◽  
Cell Responses ◽  
Boost Immunization ◽  
Memory Cd8 T Cell

CD4+ T cell help is important for the generation of CD8+ T cell responses. We used depleting anti-CD4 mAb to analyze the role of CD4+ T cells for memory CD8+ T cell responses after secondary infection of mice with the intracellular bacterium Listeria monocytogenes, or after boost immunization by specific peptide or DNA vaccination. Surprisingly, anti-CD4 mAb treatment during secondary CD8+ T cell responses markedly enlarged the population size of antigen-specific CD8+ T cells. After boost immunization with peptide or DNA, this effect was particularly profound, and antigen-specific CD8+ T cell populations were enlarged at least 10-fold. In terms of cytokine production and cytotoxicity, the enlarged CD8+ T cell population consisted of functional effector T cells. In depletion and transfer experiments, the suppressive function could be ascribed to CD4+CD25+ T cells. Our results demonstrate that CD4+ T cells control the CD8+ T cell response in two directions. Initially, they promote the generation of a CD8+ T cell responses and later they restrain the strength of the CD8+ T cell memory response. Down-modulation of CD8+ T cell responses during infection could prevent harmful consequences after eradication of the pathogen.

scholarly journals Functional Characterization of Class Ia- and Non-Class Ia-RestrictedChlamydia-Reactive CD8+T Cell Responses in Humans

2003 ◽  
Vol 171 (8) ◽  
pp. 4278-4286 ◽  
Author(s):  
Ana L. Gervassi ◽  
Peter Probst ◽  
Walter E. Stamm ◽  
Jeanne Marrazzo ◽  
Kenneth H. Grabstein ◽  
...  
Keyword(s):  
T Cell ◽  
Functional Characterization ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Cell Responses
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