Use of an insect cell culture growth medium to isolate bacteria from horses with effusive, fibrinous pericarditis: A preliminary study

2007 ◽  
Vol 121 (1-2) ◽  
pp. 177-181 ◽  
Author(s):  
Samuel L. Jones ◽  
Amy Valenzisi ◽  
Sushama Sontakke ◽  
Kimberly A. Sprayberry ◽  
Ricardo Maggi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Growth Medium ◽  
Insect Cell ◽  
Insect Cell Culture ◽  
Preliminary Study ◽  
Culture Growth ◽  
Cell Culture Growth

scholarly journals Primary insect cell culture from total embryo and embryonic brain tissue of Periplaneta americana: A preliminary study

2015 ◽  
Vol 67 (4) ◽  
pp. 1203-1208
Author(s):  
Seçkin Soya ◽  
Hüseyin Can ◽  
Mehmet Yıkılmaz
Keyword(s):  
Cell Culture ◽  
Brain Tissue ◽  
Insect Cell ◽  
Periplaneta Americana ◽  
Single Cells ◽  
Petri Dish ◽  
Insect Cell Culture ◽  
Embryonic Brain ◽  
Network Systems ◽  
Preliminary Study

The aim of this preliminary study was to establish a primary insect cell culture from total embryos and embryonic brain tissues of Periplaneta americana, collected from Izmir, Turkey. Cells were cultured at 29?C in Grace?s insect medium for one month. In the embryonic brain tissue culture, single cells and cell clumps containing spherical and ovoid as well as dividing cells were observed. Single bipolar neurons were detected after 4 days in culture. Network systems comprised of bipolar neurons were observed on the 5th day of incubation. In addition, presumably glia cells were observed in the embryonic brain culture. In the total embryo culture, the cell population exhibited variable morphologies, including spherical, spindle-like, polygonal and giant cells after nearly 20 days; the culture covered almost half of the Petri dish area within 30 days. This preliminary study associated with Periplaneta americana primary cell culture is the first of its kind in Turkey. These results should contribute to the development of new insect cell lines that are indigenous to Turkey.

scholarly journals Reproduction of Leishmania (Leishmania ) infantum chagasi in conditioned cell culture growth medium

2006 ◽  
Vol 48 (3) ◽  
pp. 147-150 ◽  
Author(s):  
Yeda L. Nogueira ◽  
Eunice A.B. Galati
Keyword(s):  
Cell Culture ◽  
Growth Medium ◽  
Growth Kinetics ◽  
Leishmania Infantum ◽  
Low Cost ◽  
High Efficacy ◽  
Diagnostic Kits ◽  
Leishmania Infantum Chagasi ◽  
Culture Growth ◽  
Cell Culture Growth

Leishmanias can be produced by inoculation in conditioned McCoy cell culture growth medium (CGM). Leishmania (Leishmania) infantum chagasi (100 parasites) grown in NNN medium was inoculated in 2.5 mL CGM, kept in plates (24 wells) and its multiplication was observed for five days (120 hours). After day 5, the medium was saturated with the flagellate forms of the parasite (promastigotes). The reproduction of the leishmanias was observed every 24 hours and the number of parasites was calculated by counting the parasites in a drop of 10 µ L and photomicrographied. So the number of Leishmanias was adjusted to 1 mL volume. The advantage of the technique by isolation of Leishmania in CGM demonstrated in this study is its low cost and high efficacy even with a small quantity of parasites (10² promastigotes) used as inoculum. Additionally, isolation of the leishmania can be obtained together with an increase in their density (180 times) as observed by growth kinetics, within a shorter time. These results justify the use of this low-cost technique for the isolation and investigation of the behavior and multiplication of Leishmania both in vertebrates and invertebrates, besides offering means of obtaining antigens, whether whole antigens (leishmanias) or the soluble antigens produced by the parasites which may be useful for the production of new diagnostic kits.

Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles

2007 ◽  
Vol 103 (6) ◽  
pp. 572-574 ◽  
Author(s):  
Takuya Shishido ◽  
Masaru Muraoka ◽  
Hideki Yamaji ◽  
Akihiko Kondo ◽  
Hideki Fukuda
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Insect Cell Culture ◽  
Biomass Support Particles

Effects of NDA, a new plant growth retardant, on cell culture growth ofZea mays L.

1983 ◽  
Vol 2 (1-4) ◽  
pp. 19-29 ◽  
Author(s):  
Klaus Grossmann ◽  
Wilhelm Rademacher ◽  
Johannes Jung
Keyword(s):  
Cell Culture ◽  
Plant Growth ◽  
Growth Retardant ◽  
Culture Growth ◽  
Cell Culture Growth

Medium design for insect cell culture

1996 ◽  
Vol 20 (1-3) ◽  
pp. 57-70 ◽  
Author(s):  
Ernst-J�rgen Schlaeger
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Insect Cell Culture ◽  
Medium Design
Sign in / Sign up

Export Citation Format

Share Document