A Plea for Standardized Nuclear Markers in Metazoan DNA Taxonomy

2020 ◽  
Vol 35 (4) ◽  
pp. 336-345 ◽  
Author(s):  
Jonas Eberle ◽  
Dirk Ahrens ◽  
Christoph Mayer ◽  
Oliver Niehuis ◽  
Bernhard Misof
Keyword(s):  
Nuclear Markers ◽  
Dna Taxonomy

scholarly journals Hierarchical Analysis of Genetic Structure in Native Fire Ant Populations: Results From Three Classes of Molecular Markers

Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 643-655 ◽  
Author(s):  
Kenneth G Ross ◽  
Michael J B Krieger ◽  
D DeWayne Shoemaker ◽  
Edward L Vargo ◽  
Laurent Keller
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Solenopsis Invicta ◽  
Large Scale ◽  
Fire Ant ◽  
Nuclear Markers ◽  
Social Forms ◽  
Native Populations ◽  
Nest Level ◽  
Nuclear Differentiation

We describe genetic structure at various scales in native populations of the fire ant Solenopsis invicta using two classes of nuclear markers, allozymes and microsatellites, and markers of the mitochondrial genome. Strong structure was found at the nest level in both the monogyne (single queen) and polygyne (multiple queen) social forms using allozymes. Weak but significant microgeographic structure was detected above the nest level in polygyne populations but not in monogyne populations using both classes of nuclear markers. Pronounced mitochondrial DNA (mtDNA) differentiation was evident also at this level in the polygyne form only. These microgeographic patterns are expected because polygyny in ants is associated with restricted local gene flow due mainly to limited vagility of queens. Weak but significant nuclear differentiation was detected between sympatric social forms, and strong mtDNA differentiation also was found at this level. Thus, queens of each form seem unable to establish themselves in nests of the alternate type, and some degree of assortative mating by form may exist as well. Strong differentiation was found between the two study regions usinga all three sets of markers. Phylogeographic analyses of the mtDNA suggest that recent limitations on gene flow rather than longstanding barriers to dispersal are responsible for this large-scale structure.

The intellectual content of taxonomy: a comment on DNA taxonomy

2003 ◽  
Vol 18 (2) ◽  
pp. 65-66 ◽  
Author(s):  
Diana Lipscomb ◽  
N Platnick ◽  
Q Wheeler
Keyword(s):  
Dna Taxonomy ◽  
Intellectual Content

Genetic evidence for "Darwinian" selection at the molecular level. III. The effect of the suppressive factor on nuclearly and cytoplasmically inherited chloramphenicol resistance in S. cerevisiae

1972 ◽  
Vol 18 (1) ◽  
pp. 1-7 ◽  
Author(s):  
G. H. Rank ◽  
N. T. Bech-Hansen
Keyword(s):  
Deoxyribonucleic Acid ◽  
Molecular Level ◽  
Genetic Evidence ◽  
Nuclear Markers ◽  
Chloramphenicol Resistance ◽  
Darwinian Selection ◽  
Resistance Markers ◽  
Abnormal Mitochondria

Four nuclear and two cytoplasmic chloramphenicol-resistance markers were selected in S. cerevisiae. The effect of the suppressive factor (abnormal mitochondria deoxyribonucleic acid) on the inheritance of these markers was studied. Nuclear markers were shown to be unaffected by the suppressive factor, in contrast to the loss of cytoplasmically inherited chloramphenicol resistance upon the generation of the suppressive factor.

Transformation of protoplasted yeast cells is directly associated with cell fusion

1984 ◽  
Vol 4 (4) ◽  
pp. 771-778
Author(s):  
S Harashima ◽  
A Takagi ◽  
Y Oshima
Keyword(s):  
Cell Fusion ◽  
Mating Type ◽  
Nuclear Fusion ◽  
Yeast Cells ◽  
Nuclear Markers ◽  
Type Locus ◽  
Mating Type Locus ◽  
Yeast Protoplasts ◽  
Yeast Plasmids ◽  
Binominal Distribution

The frequency of cell fusion during transformation of yeast protoplasts with various yeast plasmids with a chromosome replicon (YRp or YCp) or 2 mu DNA (YEp) was estimated by two methods. In one method, a mixture of protoplasts of two haploid strains with identical mating type and complementary auxotrophic nuclear markers with or without cytoplasmic markers was transformed. When the number of various phenotypic classes of transformants for the nuclear markers was analyzed by equations derived from binominal distribution theory, the frequency of nuclear fusion among the transformants was 42 to 100% in transformations with the YRp or YCp plasmids and 28 to 39% with the YEp plasmids. In another method, a haploid bearing the sir mutation, which allows a diploid (or polyploid) homozygous for the MAT (mating type) locus to sporulate by the expression of the silent mating-type loci HML and HMR, was transformed with the plasmids. Sporulation ability was found in 43 to 95% of the transformants with the YRp or YCp plasmids, and 26 to 31% of the YEp transformants. When cytoplasmic mixing was included with the nuclear fusion, 96 to 100% of the transformants were found to be cell fusants. Based upon these observations, we concluded that transformation of yeast protoplasts is directly associated with cell fusion.

Five new deep-sea species of nudibranchs (Gastropoda: Heterobranchia: Cladobranchia) from the Northeast Pacific

Zootaxa ◽  
2018 ◽  
Vol 4526 (4) ◽  
pp. 401 ◽  
Author(s):  
ÁNGEL VALDÉS ◽  
LONNY LUNDSTEN ◽  
NERIDA G. WILSON
Keyword(s):  
Climate Change ◽  
New Species ◽  
Deep Sea ◽  
Undescribed Species ◽  
Invertebrate Community ◽  
Nuclear Markers ◽  
Mitochondrial Coi ◽  
Northeast Pacific ◽  
Northeastern Pacific ◽  
System Information

Increased exploration of northeastern Pacific deep-sea habitats has revealed a diverse and often poorly-known invertebrate community, including a number of undescribed species of nudibranchs studied herein. We used morphology to distinguish several new species from their congeners, and generated data where possible for mitochondrial (COI, 16S) and nuclear markers (H3) to place them in a phylogenetic context. We described here Tritonia nigritigris sp. nov., Dendronotus claguei sp. nov., Ziminella vrijenhoeki sp. nov., Cuthona methana sp. nov., Aeolidia libitinaria sp. nov. and redescribed Zeusia herculea (Bergh, 1894). Another species of Tritonia is described but not named due to the absence of reproductive system information. Although there are difficulties in collection from deep-sea habitats, only two of our new species are known from single specimens. As with many other deep-sea regions, we expect the number of new species from this region to increase with further exploration. Because the deep regions of the northeast Pacific are particularly vulnerable to the effects of decreasing oxygenation due to climate change, we consider that documenting this fauna has some level of urgency. 

Uncovering cryptic diversity in Aspidomorphus (Serpentes: Elapidae): Evidence from mitochondrial and nuclear markers

2010 ◽  
Vol 54 (2) ◽  
pp. 405-416 ◽  
Author(s):  
Genevieve A. Metzger ◽  
Fred Kraus ◽  
Allen Allison ◽  
Christopher L. Parkinson
Keyword(s):  
Cryptic Diversity ◽  
Nuclear Markers ◽  
Mitochondrial And Nuclear Markers

Nuclear markers reveal that inter-lake cichlids’ similar morphologies do not reflect similar genealogy

2006 ◽  
Vol 40 (2) ◽  
pp. 383-388 ◽  
Author(s):  
Daud Kassam ◽  
Shingo Seki ◽  
Michio Hori ◽  
Kosaku Yamaoka
Keyword(s):  
Nuclear Markers
Sign in / Sign up

Export Citation Format

Share Document