Should Preconditioning Hyperbaric Oxygenation Protect the Liver Against Ischemia–Reperfusion Injury? An Experimental Study in a Rat Model

2014 ◽  
Vol 46 (1) ◽  
pp. 56-62 ◽  
Author(s):  
D.M. Losada ◽  
M.E. Jordani ◽  
M.C. Jordani ◽  
M.A.N.C. Piccinato ◽  
C.F. Fina ◽  
...  
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Hyperbaric Oxygenation ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion

scholarly journals The Effect of Erythropoietin on Testosterone Levels During Ischemia Reperfusion Injury in Rats

2016 ◽  
Vol 22 (4) ◽  
pp. 264-269
Author(s):  
C. Tsompos ◽  
C. Panoulis ◽  
K. Toutouzas ◽  
Triantafyllou Aggeliki ◽  
G. Zografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Study Time ◽  
Combined Effect ◽  
Short Term ◽  
Testosterone Levels ◽  
Short Term Effects

Abstract Objective: This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in an adrenal ischemia-reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean testosterone levels (T). Materials and methods: 40 rats of mean weight 247.7 g were used in the study. T levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results: Erythropoietin administration non significantly increased the testosterone levels by 71.21%+44.19% (p=0.1080). Reperfusion time non-significantly decreased the testosterone levels by 65.17%+44.45% (p=0.0792). However, erythropoietin administration and reperfusion time together produced a non-significant combined effect in increasing the testosterone levels by 27.65%+27.21% (p= 0.3006). Conclusions: Erythropoietin administration whether it interacted or not with reperfusion time has increasing non significant short-term effects on testosterone levels. Perhaps, a longer study time or a higher Epo dose, may reveal clearer and more significant effects.

The Effect of Erythropoietin on Amylase Levels during Ischemia-Reperfusion Injury in Rats

2016 ◽  
Vol 50 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Constantinos Tsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
George Zografos ◽  
Apostolos Papalois
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Combined Effect ◽  
Short Term ◽  
Baseline Values

ABSTRACT The aim of this experimental study was to examine the effect of erythropoietin (EPO) on rat model and particularly in an ischemia-reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean amylase levels. Materials and methods Forty rats of mean weight 247.7 gm were used in the study. Amylase levels were measured at 60 minutes (groups A and C) and at 120 minutes (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results Erythropoietin administration kept non-significantly increased the A levels by 5.04 ± 6.12% (p = 0.3831). Reperfusion time kept non-significantly increased the A levels by 10.08 ± 5.95% (p = 0.0615). However, EPO administration and reperfusion time together produced a non-significant combined effect in keeping increased the A levels by 4.36 ± 3.65% (p = 0.2258) Conclusion Erythropoietin administration, reperfusion time and their interaction kept non-significantly short-term increased the amylase levels. The restorating effect of Epo is satisfactory, since it reduced the discrepancy from baseline values at non-significant level. How to cite this article Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The Effect of Erythropoietin on Amylase Levels during Ischemia-Reperfusion Injury in Rats. J Postgrad Med Edu Res 2016;50(1):18-21.

scholarly journals The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

2017 ◽  
Vol 11 ◽  
Author(s):  
Constantinos Tsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
Aggeliki Triantafyllou ◽  
George Zografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Renal Function ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Serum Levels ◽  
Renal Ischemia

This experimental study examined the effect of the antioxidant drug U-74389G, on a rat model and particularly in a renal ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean urea levels. Forty rats of mean weight 231.875 g were used in the study. Urea levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. U-74389G administration significantly decreased the predicted urea levels by 11.35%+2.73% (P=0.0001). Reperfusion time non-significantly increased the predicted urea levels by 2.26%+3.29% (P=0.4103). However, U-74389G administration and reperfusion time together significantly decreased the predicted urea levels by 6.31%+1.70% (P=0.0005). U-74389G administration whether it interacted or not with reperfusion time has significant decreasing effect on the urea serum levels, reflecting a respective renal function augmentation.

scholarly journals The Effect of the Antioxidant Drug U-74389G on Uric Acid Levels during Ischemia Reperfusion Injury in Rats

2016 ◽  
Vol 17 (3) ◽  
pp. 247-250
Author(s):  
Constantinos Tsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
Aggeliki Triantafyllou ◽  
George Zografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Uric Acid ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Tubular Epithelial Cells ◽  
Renal Ischaemia ◽  
Ischaemia Reperfusion ◽  
Anti Oxidant

AbstractThis experimental study examined the effect of the anti-oxidant drug U-74389G in a rat model using a renal ischaemia-reperfusion (IR) protocol. The effects of the molecule were studied biochemically by assessing mean serum uric acid levels (SUA). In total, 40 rats (mean weight = 231.875 g) were used in the study. SUA levels were measured at 60 min of reperfusion for groups A and C and at 120 min of reperfusion for groups B and D. The drug U-74389G was administered only in groups C and D. U-74389G administration non-significantly increased the SUA levels by 15.43%±9.10% (p=0.096) at the representative endpoint of 1.5 h. The reperfusion time non-significantly decreased the SUA levels by 13.61%±9.18% (p=0.126). However, the interaction of U-74389G administration and reperfusion time non-significantly increased the SUA levels by 4.78%±5.64% (p= 0.387). Whether it interacted with the reperfusion time, U-74389G administration non-significantly increased SUA levels. It seems that U-74389G cannot reverse injury to IR tubular epithelial cells within 2 hours.

scholarly journals THE EFFECT OF THE ANTIOXIDANT DRUG “U-74389G” ON TOTAL PROTEIN LEVELS DURING ISCHEMIA REPERFUSION INJURY IN RATS

2015 ◽  
Vol 14 (1) ◽  
pp. 30-41
Author(s):  
Constantinos Tsompos ◽  
◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
George Zografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Beneficial Effect ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Total Protein ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Protein Levels

The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G”, on rat model and particularly in an ischemia – reperfusion protocol. The beneficial effect or non-effectiveness of that molecule were studied biochemically using blood mean total protein levels. Materials and methods. 40 rats of mean weight 231.875 gr were used in the study. Total protein levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D), A and B without but C and D with U-74389G administration. Results were that U-74389G administration significantly decreased the predicted TP levels by 7.34% + 1.76% (P = 0.000). Reperfusion time non-significantly increased the predicted TP levels by 1.46% + 2.12% (p = 0.410). However, U-74389G administration and reperfusion time together significantly decreased the TP levels by 4.08% + 1.10% (P = 0.000). Conclusions are that U-74389G administration interacted or not with reperfusion time significantly decreases the total protein levels.

scholarly journals The Effect of the Antioxidant Drug “U-74389G” on Creatinine Levels during Ischemia Reperfusion Injury in Rats

2015 ◽  
Vol 9 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Constantinos Τsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Τοutouzas ◽  
George Ζografos ◽  
Apostolos Papalois
Keyword(s):  
Experimental Study ◽  
Renal Function ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Combined Effect ◽  
Short Term ◽  
Creatinine Excretion ◽  
Blood Creatinine

Objective: The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G” on a rat model using an ischemia reperfusion protocol. The effect of U-74389G was studied biochemically by measuring mean blood creatinine levels. Materials and Methods: Forty rats were used in the study. Creatinine levels were measured at 60 min of reperfusion (groups A and C) or at 120 min of reperfusion (groups B and D), where groups A and B were controls and groups C and D received U-74389G administration. Results: U-74389G administration significantly decreased the predicted creatinine levels by 21.02 ± 5.06% (p = 0.0001). Reperfusion time non-significantly increased the predicted creatinine levels by 4.20 ± 6.12% (p = 0.4103). However, U-74389G administration and reperfusion time together produced a significant combined effect in decreasing the predicted creatinine levels by 11.69 ± 3.16% (p = 0.0005). Conclusion: Independent of reperfusion time, U-74389G administration significantly decreased the creatinine levels in an ischemic rat model. This study demonstrates that short-term U-74389G administration improves renal function by increasing creatinine excretion.

Effect of morphine on ischemia-reperfusion injury: Experimental study in testicular torsion rat model

Urology ◽  
2005 ◽  
Vol 66 (6) ◽  
pp. 1338-1342 ◽  
Author(s):  
Amirali Hassanzadeh Salmasi ◽  
Azadeh Beheshtian ◽  
Seyedmehdi Payabvash ◽  
Shadpour Demehri ◽  
Mohammad Reza Ebrahimkhani ◽  
...  
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Testicular Torsion ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion

scholarly journals The Effect of the Antioxidant Drug U-74389G on Albumin Levels during Ischemia Reperfusion Injury in Rats

2016 ◽  
Vol 43 (2) ◽  
pp. 11-20 ◽  
Author(s):  
C. Τsompos ◽  
C. Panoulis ◽  
K. Τοutouzas ◽  
A. Triantafyllou ◽  
G. Ζografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Antioxidant Capacity ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia ◽  
Short Term ◽  
Short Term Effects

SummaryThis experimental study examined the effect of the antioxidant drug U-74389G on a rat model and particularly in a liver ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean albumin levels. 40 rats of mean weight 231.875 g were used in the study. Albumin levels were measured at 60th min of reperfusion (groups A and C) and at 120th min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. U-74389G administration significantly decreased the predicted albumin levels by 3.63% ± 0.87% (p = 0.0001). Reperfusion time non-significantly increased the predicted albumin levels by 0.72% ± 1.04% (p = 0.4103). However, U-74389G administration and reperfusion time together significantly decreased the predicted albumin levels by 2.02% ± 0.54% (p = 0.0005). U-74389G administration whether it interacted or not with reperfusion time has significant decreasing short - term effects on albumin levels. It seems that the antioxidant capacity is associated with albumin catabolism.

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