Immune Suppression Produced by Intrathymic Inoculation With Xenogeneic Antigen and Whole-Body γ-Irradiation in a Pig-to-Monkey Heart Transplantation Model

2010 ◽  
Vol 42 (9) ◽  
pp. 3759-3762 ◽  
Author(s):  
Q.V. Jichen ◽  
G. Chen ◽  
G. Jiang ◽  
J. Ding ◽  
W. Gao ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Immune Suppression ◽  
Whole Body ◽  
Γ Irradiation ◽  
Transplantation Model

Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents and preliminary studies of their metabolism

Parasitology ◽  
1978 ◽  
Vol 76 (2) ◽  
pp. 159-176 ◽  
Author(s):  
W. E. Gutteridge ◽  
B. Cover ◽  
Maria Gaborak
Keyword(s):  
Trypanosoma Cruzi ◽  
Blood Cells ◽  
Light And Electron Microscopy ◽  
Deae Cellulose ◽  
Whole Body ◽  
Differential Centrifugation ◽  
Γ Irradiation ◽  
Hind Limb Muscle ◽  
Radio Tracer

SummaryIsolation of blood and intracellular forms of Trypanosoma cruzi was made mainly from rats (90–110 g) which had received 580 rad of whole-body γ-irradiation not more than 24 h before subcutaneous inoculation with 107 trypomastigotes of the Sonya strain of T. cruzi. Unirradiated chinchillas (250–350 g) were, however, used for some experiments. Blood forms were isolated using a technique involving differential centrifugation to remove most of the erythrocytes and DEAE–cellulose chromatography to remove the remaining blood cells. Overall recoveries were usually in the range 30–70%. Parasites were mainly (approximately 98%) broad forms and were motile, metabolically active (as judged by respiratory and radio-tracer incorporation studies) and had lost none of their infectivity for mice. Intracellular forms were isolated from hind-limb muscle tissue. This was disrupted in an MSE tissue homogenizer and the homogenate incubated with DNase, collagenase and trypsin. Parasites, contaminated only by a few blood cells, were then obtained by differential centrifugation. For purer preparations, a terminal sucrose gradient step was used. Recoveries ranged between 40 and 70%. About 1–3% of the parasites isolated were epimastigotes and trypomastigotes; the remainder are probably best collectively termed ‘amastigotes’, though they were pointed and most had a short, free flagellum. They were undamaged as judged by light and electron microscopy and metabolically active as judged by respiratory and radio-tracer incorporation studies. However, the infectivity for mice of both these purified preparations and the initial cell homogenates could be accounted for by the epimastigotes and trypomastigotes present in them. Preliminary biochemical studies with isolated parasites have shown that blood, intracellular and culture forms of T. cruzi have a respiratory system which is in part sensitive to CN- and that all forms synthesize nucleic acids and proteins when incubated in vitro. There appears, however, to be a lack of DNA synthesis in blood stages, and thus it is not surprising that these forms do not divide.

scholarly journals Regulatory T Cells in γ Irradiation-Induced Immune Suppression

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39092 ◽  
Author(s):  
Hugh I. McFarland ◽  
Montserrat Puig ◽  
Lucja T. Grajkowska ◽  
Kazuhide Tsuji ◽  
Jay P. Lee ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Immune Suppression ◽  
Γ Irradiation

scholarly journals Hepatic Gene Expression Changes in Rats Internally Exposed to Radioactive 56MnO2 Particles at Low Doses

2021 ◽  
Vol 43 (2) ◽  
pp. 758-766
Author(s):  
Bakhyt Ruslanova ◽  
Zhaslan Abishev ◽  
Nailya Chaizhunussova ◽  
Dariya Shabdarbayeva ◽  
Sholpan Tokesheva ◽  
...  
Keyword(s):  
Atomic Bomb ◽  
Kinase Inhibitor ◽  
Biological Effects ◽  
Nuclear Factor Κb ◽  
Internal Exposure ◽  
Whole Body ◽  
Control Group ◽  
Gene Expressions ◽  
Γ Irradiation ◽  
Γ Rays

We have studied the biological effects of the internal exposure to radioactive manganese-56 dioxide (56MnO2), the major radioisotope dust found in soil after atomic bomb explosions. Our previous study of blood chemistry indicated a possible adverse effect of 56MnO2 on the liver. In the present study, we further examined the effects on the liver by determining changes in hepatic gene expressions. Male Wistar rats were exposed to 56MnO2 particles (three groups with the whole-body doses of 41, 90, and 100 mGy), stable MnO2 particles, or external 60Co γ-rays (2 Gy), and were examined together with the non-treated control group on postexposure day 3 and day 61. No histopathological changes were observed in the liver. The mRNA expression of a p53-related gene, the cyclin-dependent kinase inhibitor 1A, increased in 56MnO2 as well as in γ-ray irradiated groups on postexposure day 3 and day 61. The expression of a stress-responsive gene, nuclear factor κB, was also increased by 56MnO2 and γ-rays on postexposure day 3. However, the expression of cytokine genes (interleukin-6 or chemokine ligand 2) or fibrosis-related TGF-β/Smad genes (Tgfb1, Smad3, or Smad4) was not altered by the exposure. Our data demonstrated that the internal exposure to 56MnO2 particles at less than 0.1 Gy significantly affected the short-term gene expressions in the liver in a similar manner with 2 Gy of external γ-irradiation. These changes may be adaptive responses because no changes occurred in cytokine or TGF-β/Smad gene expressions.

Efficacy of Mycophenolic Acid Combined With KRP-203, a Novel Immunomodulator, in a Rat Heart Transplantation Model

2006 ◽  
Vol 25 (3) ◽  
pp. 302-309 ◽  
Author(s):  
Chihiro Suzuki ◽  
Masafumi Takahashi ◽  
Hajime Morimoto ◽  
Atsushi Izawa ◽  
Hirohiko Ise ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Mycophenolic Acid ◽  
Rat Heart ◽  
Transplantation Model

A NEW SYNTHETIC IMMUNOMODULATOR, KRP-203, ABROGATES CHRONIC REJECTION IN A RAT HEART TRANSPLANTATION MODEL

2004 ◽  
Vol 78 ◽  
pp. 49
Author(s):  
H Shimizu ◽  
M Takahashi ◽  
T Murakami ◽  
T Kaneko ◽  
Y Hakamata ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Chronic Rejection ◽  
Rat Heart ◽  
Transplantation Model

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

10.3791/62007 ◽  
2021 ◽  
Author(s):  
Decheng Yin ◽  
Jian Fu ◽  
Ida Allabauer ◽  
Oliver Witzke ◽  
Song Rong ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Mouse Heart ◽  
Transplantation Model

scholarly journals Acute Monocytic Leukemia in an Irradiated Beagle

1979 ◽  
Vol 16 (2) ◽  
pp. 243-254 ◽  
Author(s):  
D. V. Tolle ◽  
T. M. Seed ◽  
T. E. Fritz ◽  
L. S. Lombard ◽  
C. M. Poole ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Whole Body ◽  
Acute Monocytic Leukemia ◽  
Monocytic Differentiation ◽  
Monocytic Leukemia ◽  
Γ Irradiation ◽  
Culture Studies ◽  
Invasive Cell ◽  
Progressive Anemia

A purebred female Beagle dog that had received 2,000 R of protracted whole-body γ-irradiation from 60Co when 14 months old had hematologic changes consistent with a myeloproliferative disorder 3 years after the termination of radiation exposure. Peripheral blood and bone marrow findings during the 7-month period before death showed progressive anemia with increased numbers of platelets; immature granulocytes, monocytes and promonocytes. A period of partial remission occurred during which time the peripheral blood was aleukemic, although there was marked thrombocytosis and abnormal erythropoiesis which was evidenced by bizarre circulating nucleated red cells, anisocytosis, poikilocytosis and Howell-Jolly bodies. The dog had a terminal crisis with marked leukocytosis, most cells in the peripheral blood being bizarre monocytes and promonocytes. Tissues obtained at necropsy showed diffuse as well as focal infiltration of the spleen, liver, lymph nodes, heart, kidney and gastrointestinal wall with immature neoplastic cells resembling monocytes and monocytic precursors. The monocytic differentiation of the invasive cell population was confirmed by morphological, cytochemical, histological, ultrastructural and in vitro cell culture studies.

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