Regulation of OX40 gene expression in graft-versus-host disease

2005 ◽  
Vol 37 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Y. Miura ◽  
C.J. Thoburn ◽  
E.C. Bright ◽  
S. Arai ◽  
A.D. Hess
Keyword(s):  
Gene Expression ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host

The relationship between HIF family gene expression in allogeneic T-cell products and acute graft-versus-host disease.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. 7026-7026
Author(s):  
Joseph Rimando ◽  
Steven Larrick ◽  
Nicholas Fowler ◽  
Luciano Castiello ◽  
Syed Abbas Ali ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host ◽  
Family Gene ◽  
The Relationship ◽  
Acute Graft

scholarly journals Increased P2X7 expression in the gastrointestinal tract and skin in a humanised mouse model of graft-versus-host disease

2020 ◽  
Vol 134 (2) ◽  
pp. 207-223 ◽  
Author(s):  
Peter Cuthbertson ◽  
Sam R. Adhikary ◽  
Nicholas J. Geraghty ◽  
Thomas V. Guy ◽  
Amirazin Hadjiashrafi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Graft Versus Host Disease ◽  
Mononuclear Cells ◽  
Early Stage ◽  
Human Peripheral Blood ◽  
Quantitative Polymerase Chain Reaction ◽  
Haematopoietic Stem Cell ◽  
Host Disease ◽  
Graft Versus Host ◽  
Nsg Mice

Abstract Background: Allogeneic haematopoietic stem cell transplantation (HSCT) is a curative therapy for blood cancers; but results in the development of graft-versus-host disease (GVHD) in up to 70% of recipients. During GVHD, tissue damage results in ATP release into the extracellular compartment activating P2X7 on antigen-presenting cells, leading to the release of pro-inflammatory cytokines and subsequent activation of donor T cells. Therefore, the aim of the present study was to examine murine (m) P2rx7 and human (h) P2RX7 gene expression in GVHD target organs of humanised mice, and further characterise disease impact in these organs. Methods: NOD-scid IL2Rγnull (NSG) mice were injected with human peripheral blood mononuclear cells (hu-PBMC-NSG mice) or phosphate-buffered saline (PBS, control). Leucocytes were assessed by flow cytometry; gene expression was measured by quantitative polymerase chain reaction (qPCR), and tissue sections examined by histology. Results: Compared with control mice, hu-PBMC-NSG mice had increased mP2rx7 and mP2rx4 expression in the duodenum, ileum and skin. hP2RX7 was expressed in all tissues examined. hu-PBMC-NSG mice also displayed increased mReg3g expression in the duodenum and ileum, despite limited histological gut GVHD. hu-PBMC-NSG mice showed histological evidence of GVHD in the skin, liver and lung. Compared with control mice, hu-PBMC-NSG mice displayed increased ear swelling. Conclusion: Combined data revealed that P2rx7 is up-regulated in gut and skin GVHD and that P2RX7 is present in target tissues of GVHD, corresponding to human leucocyte infiltration. Data also reveal increased mReg3g expression and ear swelling in hu-PBMC-NSG mice, offering new measurements of early-stage gut GVHD and skin GVHD, respectively.

Levels of soluble FasL and FasL gene expression during the development of graft-versus-host disease in DLT-treated patients

1999 ◽  
Vol 104 (4) ◽  
pp. 795-800 ◽  
Author(s):  
Hiranmoy Das ◽  
Shion Imoto ◽  
Tohru Murayama ◽  
Kazuyoshi Kajimoto ◽  
Takeshi Sugimoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host ◽  
Soluble Fasl ◽  
Fasl Gene

The Impact of Age and Body Fat on Graft-Versus-Host Disease (GVHD) in Mice

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1100-1100
Author(s):  
Chien-Chun Steven Pai ◽  
Mingyi Chen ◽  
Lam Khuat ◽  
Annie Mirsoian ◽  
Anthony E Zamora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Fat ◽  
Graft Versus Host Disease ◽  
Acute Gvhd ◽  
Obese Mice ◽  
Gi Tract ◽  
Host Disease ◽  
Graft Versus Host ◽  
Aged Mice ◽  
Tnf Α

Abstract Allogeneic hematopoietic stem cell transplantation (HSCT) is a potentially curative procedure performed for a variety of hematological diseases. Patient populations undergoing HSCT are generally skewed towards either young or old age due to the pathogenesis of hematological cancers. Several retrospective studies have identified age as one of the risk factors that correlate with treatment related mortality (TRM), graft versus host disease (GVHD) and tumor relapse. Aging is characterized by a gradual decline in immune cell function, known as immune senescence, yet is also hallmarked by a chronic, low-grade proinflammatory phenotype termed “inflammaging”. We hypothesized that aged animals develop more severe GVHD as a result of this inflammaging phenomenon. To investigate this, we first transplanted donor cells from B10.D2 mice (H-2d) into either young (< 3 months old) or aged (>15 months old) BALB/c (H-2d) recipients. While young recipients developed typical sclerodermatous chronic GVHD and died by 56 days post transplantation, aged mice developed severe acute GVHD and died at Day 7. Upon pathological examination, aged mice displayed massive lymphocytic infiltration associated with tissue necrosis in the gastrointestinal (GI) tract. Among several cytokines examined, elevated levels of serum TNF-α (97.89±9.83 versus 68.29±1.07 pg/ml, respectively) were observed in aged animals compared to young counterparts. Similarly, TNF-α and IL-6 gene expression levels were also increased in GI tract tissues. Additionally, we found greater frequencies of splenocyte derived TNF-α+ macrophages (CD45+CD19-F4/80+/CD11b+) in aged animals compared to young animals (26.2±1.00% versus 17.233±1.25%, respectively; P< 0.001) following allogeneic HSCT. Macrophage depletion using liposomal clodronate reduced serum TNF-α levels (97.89 ±9.83 versus 57.17±2.86 pg/ml) in aged mice following HSCT. We observed that aged mice had markedly higher levels of visceral body fat compared to young mice. Based on the similarities in the inflammatory status between aged and obese animals, we next sought to verify whether the severity of GVHD can also be attributed to obesity. Eight week old recipient BALB/c mice were maintained on either a low fat diet (10% calories from fat) or a high fat diet (60% calories from fat) for three months. Each cohort of mice then underwent HSCT, following a conditional regimen of total body irradiation (Cs; 800 cGy) and adoptive transfer from donor B10.D2 mice. In line with the results observed from aged recipients, diet induced obese (DIO) mice died at Day 7 and demonstrated a severe acute GVHD response in the GI tract compared to lean mice based on histo-pathological scores correlating with significantly increased TNF-α and IL-6 gene expression in the GI tract. Flow analysis revealed an increase in total numbers of CD8+ T cells infiltrating in the GI tract in obese mice compared to lean mice (0.229± 0.025 x 106 versus 0.071± 0.010 x 106; P<0.01). In addition, obese mice demonstrated an increase in total numbers of TNF-a+ macrophages (CD45+F4/80+CD11b+TNF-α +; 5.18± 1.09 x 104 versus 1.31± 0.75 x 104 ; P<0.05) in the visceral fat tissues. Overall, these data implicate that age and body fat can predispose to severe acute GVHD, which is associated with increased production of proinflammatory cytokines mediated by dysregulated macrophages. Disclosures No relevant conflicts of interest to declare.

scholarly journals Kinetics of Gene Expression in Murine Cutaneous Graft-versus-Host Disease

2004 ◽  
Vol 164 (6) ◽  
pp. 2189-2202 ◽  
Author(s):  
Philip B. Sugerman ◽  
Sara B. Faber ◽  
Lucy M. Willis ◽  
Aleksandra Petrovic ◽  
George F. Murphy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host ◽  
Kinetics Of

Interferon-gamma gene expression during acute graft-versus-host disease: relationship to MHC induction and tissue injury

1999 ◽  
Vol 189 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Nollaig A. Parfrey ◽  
Amr El-Sheikh ◽  
Elizabeth A. Monckton ◽  
Sandra M. Cockfield ◽  
Philip F. Halloran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Interferon Gamma ◽  
Graft Versus Host Disease ◽  
Tissue Injury ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

A Preliminary Gene Expression Profile of Acute Graft-Versus-Host Disease

2008 ◽  
Vol 17 (5) ◽  
pp. 489-494 ◽  
Author(s):  
Matthew P. Buzzeo ◽  
Jie Yang ◽  
George Casella ◽  
Vijay Reddy
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft
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