Raman imaging goes to high resolution

2011 ◽  
Vol 30 (11) ◽  
pp. 1702-1703
Author(s):  
Harald Fischer
Keyword(s):  
High Resolution ◽  
Raman Imaging

High-resolution, high-speed confocal Raman imaging

2008 ◽  
Vol 48 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Thomas Dieing ◽  
Olaf Hollricher
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Raman Imaging ◽  
Confocal Raman

scholarly journals The potential of Raman microscopy and Raman imaging in plant research

2007 ◽  
Vol 21 (2) ◽  
pp. 69-89 ◽  
Author(s):  
Notburga Gierlinger ◽  
Manfred Schwanninger
Keyword(s):  
Raman Spectroscopy ◽  
Cell Wall ◽  
High Resolution ◽  
Structural Information ◽  
Plant Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Imaging ◽  
Hierarchical Level ◽  
Inorganic Substances ◽  
Fourier Transform Raman Spectroscopy

To gain a better understanding on structure, chemical composition and properties of plant cells, tissues and organs several microscopic, chemical and physical methods have been applied during the last years. However, a knowledge gap exists about the location, quantity and structural arrangement of molecules in the native sample or what happens on the molecular level when samples are chemically or mechanically treated or how they respond to mechanical stress. These questions need to be answered to optimise utilization of plants in food industry and pharmacy and to understand structure-function relationships of plant cells to learn from natures unique. Advances in combining microscopy with Raman spectroscopy have tackled this problem in a non-invasive way and provide chemical and structural informationin situwithout any staining or complicated sample preparation. In this review the different Raman techniques (e.g. near infrared Fourier Transform Raman spectroscopy (NIR-FT), resonance Raman spectroscopy, surface-enhanced Raman spectroscopy) are briefly described before approaches in plant science are summarised. Investigations on structural cell wall components, valuable plant substances, metabolites and inorganic substances are included with emphasis on Raman imaging. The introduction of the NIR-FT-Raman technique led to many applications on green plant material by eliminating the problem of sample fluorescence. For mapping and imaging of whole plant organs (seeds, fruits, leaves) the lateral resolution (~10μm) of the NIR-FT technique is adequate, whereas for investigations on the lower hierarchical level of cells and cell walls the high resolution gained with a visible laser based system is needed. Examples on high resolution Raman imaging are given on wood cells, showing that changes in chemistry and orientation can be followed within and between different cell wall layers having dimensions smaller than 1 μm. In addition imaging the distribution of amorphous silica is shown on horsetail tissue, including an area scan from a cross section as well as a depth profiling within a silica rich knob of the outer stem wall.

scholarly journals In situ measurement of absolute concentrations by Normalized Raman Imaging

2019 ◽  
Author(s):  
Seungeun Oh ◽  
ChangHee Lee ◽  
Wenlong Yang ◽  
Ang Li ◽  
Chongzhao Ran ◽  
...  
Keyword(s):  
Light Scattering ◽  
High Resolution ◽  
Stimulated Raman Scattering ◽  
Water Concentration ◽  
Raman Imaging ◽  
Absolute Concentration ◽  
Tissue Samples ◽  
Microscopy Method ◽  
Absolute Concentrations

AbstractWe introduce Normalized Raman Imaging (NoRI), a Stimulated Raman Scattering (SRS) microscopy method that computationally removes the effect of tissue light scattering. NoRI provides high resolution measurements of the absolute concentration of total protein, total lipid and water from live or fixed thick tissue samples with single cell resolution, and can also be applied to other Raman bands. NoRI enables study of the protein, lipid, and water concentration variation associated with development and diseases.

scholarly journals Insights into the chemical composition of Equisetum hyemale by high resolution Raman imaging

Planta ◽  
2007 ◽  
Vol 227 (5) ◽  
pp. 969-980 ◽  
Author(s):  
Notburga Gierlinger ◽  
Lanny Sapei ◽  
Oskar Paris
Keyword(s):  
Chemical Composition ◽  
High Resolution ◽  
Raman Imaging ◽  
Equisetum Hyemale

Distribution of Polymorphic Crystals in the Ring-Banded Spherulites of Poly(butylene adipate) Studied Using High-Resolution Raman Imaging

2017 ◽  
Vol 50 (8) ◽  
pp. 3377-3387 ◽  
Author(s):  
Mengfan Wang ◽  
Sanpon Vantasin ◽  
Jiping Wang ◽  
Harumi Sato ◽  
Jianming Zhang ◽  
...  
Keyword(s):  
High Resolution ◽  
Raman Imaging ◽  
Polymorphic Crystals ◽  
Banded Spherulites

scholarly journals A microfluidic chip for high resolution Raman imaging of biological cells

RSC Advances ◽  
2015 ◽  
Vol 5 (61) ◽  
pp. 49350-49355 ◽  
Author(s):  
Barbara M. Liszka ◽  
Hoon Suk Rho ◽  
Yoonsun Yang ◽  
Aufried T. M. Lenferink ◽  
Leon W. M. M. Terstappen ◽  
...  
Keyword(s):  
High Resolution ◽  
Microfluidic Chip ◽  
Raman Imaging ◽  
Biological Cells ◽  
Imaging Spectrometer ◽  
Confocal Raman

A microfluidic chip was designed, prepared and tested for integration with a confocal Raman imaging spectrometer with the specific purpose of enabling studies of individual biological cells.

High-resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

2014 ◽  
Vol 46 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Katarzyna M. Marzec ◽  
Anna Rygula ◽  
Bayden R. Wood ◽  
Stefan Chlopicki ◽  
Malgorzata Baranska
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Spatial Location ◽  
Raman Imaging

scholarly journals High-resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

2015 ◽  
Vol 47 (2) ◽  
pp. 252-252 ◽  
Author(s):  
Katarzyna M. Marzec ◽  
Anna Rygula ◽  
Bayden R. Wood ◽  
Stefan Chlopicki ◽  
Malgorzata Baranska
Keyword(s):  
High Resolution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Spatial Location ◽  
Raman Imaging
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