Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

Toxicology ◽  
2007 ◽  
Vol 231 (2-3) ◽  
pp. 100-101
Author(s):  
D STEVENSON ◽  
C MORGAN ◽  
L MCLELLAN ◽  
M GRANT
Keyword(s):  
Reduced Glutathione ◽  
Glutathione Synthesis ◽  
Monolayer Cultures ◽  
Hepatocyte Monolayer

Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

2007 ◽  
Vol 21 (3) ◽  
pp. 527-532 ◽  
Author(s):  
David J. Stevenson ◽  
Caroline Morgan ◽  
Lesley I. McLellan ◽  
M. Helen Grant
Keyword(s):  
Reduced Glutathione ◽  
Glutathione Synthesis ◽  
Monolayer Cultures ◽  
Hepatocyte Monolayer

Effect of Epidermal Growth Factor on Adult Rat Hepatocyte Monolayer Cultures

1980 ◽  
Vol 38 ◽  
pp. 778-779
Author(s):  
W. A. Samsonoff ◽  
R. Jansing
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Function ◽  
Parenchymal Cell ◽  
Male Rats ◽  
Monolayer Cultures ◽  
Epidermal Growth ◽  
And Function ◽  
Hepatocyte Monolayer

Primary hepatocyte monolayer cultures have proven useful for in vitro study of parenchymal cell function. Unfortunately these cultures lose liver function rapidly and are often short-lived. Modified subtrates (1,2) can overcome some of these problems, but they produce fewer viable cells. Specific media supplements can also prolong cellular function. Epidermal growth factor (EGF) for example, significantly affects the structure and function of cells in culture (3). In this study we determined the effect of EGF on the ultrastructure and function of hepatocytes from Fisher 344 adult male rats. They were maintained as primary monolayer cultures in Leibovitz L-15 medium with supplements.

scholarly journals Escherichia coli Resistance to Chlorine and Glutathione Synthesis in Response to Oxygenation and Starvation

1999 ◽  
Vol 65 (12) ◽  
pp. 5600-5603 ◽  
Author(s):  
Sébastien Saby ◽  
Pierre Leroy ◽  
Jean-Claude Block
Keyword(s):  
Escherichia Coli ◽  
Parent Strain ◽  
Reduced Glutathione ◽  
Glutathione Synthesis ◽  
E Coli ◽  
Oxygenation Rate

ABSTRACT Reduced glutathione (GSH) levels and resistance to chlorine were measured for two isogenic Escherichia coli strains stressed by oxygenation and/or starvation. The E. coli mutant deficient in GSH was not more sensitive to the oxidant than its parent strain when the bacteria were cultured with a low oxygenation rate. Starvation or oxygenation increased the resistance of the parent strain to chlorine, while the resistance of the deficient strain remained unchanged.

scholarly journals THE INACTIVATION OF ENTEROVIRUS INFECTIVITY BY THE SULFHYDRYL REAGENT p-CHLOROMERCURIBENZOATE

1961 ◽  
Vol 113 (4) ◽  
pp. 713-734 ◽  
Author(s):  
Purnell W. Choppin ◽  
Lennart Philipson
Keyword(s):  
Reduced Glutathione ◽  
Sulfhydryl Groups ◽  
Monkey Kidney ◽  
Host Cells ◽  
Kidney Cells ◽  
Thiol Compound ◽  
Sulfhydryl Reagent ◽  
Ionic Environment ◽  
Monolayer Cultures

The infectivity of several enteroviruses was inactivated by the sulfhydryl reagent p-chloromercuribenzoate (PCMB). The rate of inactivation was dependent on the ionic environment in which the reaction was carried out. Inactivation of infectivity was reversed by the thiol compound, reduced glutathione. Under certain conditions, PCMB prevented the adsorption of some enteroviruses to monolayer cultures of monkey kidney cells. The results suggest that enterovirus sulfhydryl groups are involved in the establishment of infection, and that they play a role in the adsorption of virus to host cells.

Cryopreservation of rat hepatocyte monolayer cultures

1996 ◽  
Vol 15 (1) ◽  
pp. 30-37 ◽  
Author(s):  
P. Watts ◽  
MH Grant
Keyword(s):  
Plasma Membranes ◽  
Rat Hepatocytes ◽  
Repair Process ◽  
Significant Loss ◽  
Urea Synthesis ◽  
Albumin Synthesis ◽  
Rat Hepatocyte ◽  
Monolayer Cultures ◽  
Cryopreservation Method ◽  
Hepatocyte Monolayer

1 Most previous attempts to cryopreserve hepatocytes have used suspensions stored at either - 70°C or in liquid nitrogen, and the major problem is that these do not, on subsequent thawing, attach well in culture. This limits their use in studies of drug metabolism and xenobiotic- induced toxicity. In this manuscript we demonstrate successful cryopreservation of rat hepatocytes as mono layers attached to a collagen film. 2 Monolayers can be frozen and thawed without significant loss of cells, and although damage to the internal and plasma membranes is evident immediately post-thaw, a remarkable repair process takes place over 24-48 h post-thaw. Immediately post-thaw only 10% of the cells exclude Trypan Blue, but by 48 h 80 - 90% of the thawed cells are viable, indicating that repair of the plasma membranes has taken place. 3 The cells post-thaw retain aspects of liver-specific function including cytochrome P450 content and albumin synthesis. However, cytosolic proteins are lost through the damaged membranes and, probably because of this, urea synthesis from ammonia is retained at only 25% of pre- freeze values. 4 A cryopreservation method based on adherent hepa tocytes on a collagen substrate overcomes the problems encountered with culture of cryopreserved hepatocyte suspensions, and may provide a practical means of establishing a 'bank' of hepatocytes from several donors and species.

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