Oxygen-stimulated cytochrome oxidase assembly in hepatocyte monolayer cultures

1982 ◽  
Vol 113 (1) ◽  
pp. 23-27 ◽  
Author(s):  
James F. Hare ◽  
Randall Hodges
Keyword(s):  
Cytochrome Oxidase ◽  
Monolayer Cultures ◽  
Hepatocyte Monolayer

Effect of Epidermal Growth Factor on Adult Rat Hepatocyte Monolayer Cultures

1980 ◽  
Vol 38 ◽  
pp. 778-779
Author(s):  
W. A. Samsonoff ◽  
R. Jansing
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Function ◽  
Parenchymal Cell ◽  
Male Rats ◽  
Monolayer Cultures ◽  
Epidermal Growth ◽  
And Function ◽  
Hepatocyte Monolayer

Primary hepatocyte monolayer cultures have proven useful for in vitro study of parenchymal cell function. Unfortunately these cultures lose liver function rapidly and are often short-lived. Modified subtrates (1,2) can overcome some of these problems, but they produce fewer viable cells. Specific media supplements can also prolong cellular function. Epidermal growth factor (EGF) for example, significantly affects the structure and function of cells in culture (3). In this study we determined the effect of EGF on the ultrastructure and function of hepatocytes from Fisher 344 adult male rats. They were maintained as primary monolayer cultures in Leibovitz L-15 medium with supplements.

Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

2007 ◽  
Vol 21 (3) ◽  
pp. 527-532 ◽  
Author(s):  
David J. Stevenson ◽  
Caroline Morgan ◽  
Lesley I. McLellan ◽  
M. Helen Grant
Keyword(s):  
Reduced Glutathione ◽  
Glutathione Synthesis ◽  
Monolayer Cultures ◽  
Hepatocyte Monolayer

Cryopreservation of rat hepatocyte monolayer cultures

1996 ◽  
Vol 15 (1) ◽  
pp. 30-37 ◽  
Author(s):  
P. Watts ◽  
MH Grant
Keyword(s):  
Plasma Membranes ◽  
Rat Hepatocytes ◽  
Repair Process ◽  
Significant Loss ◽  
Urea Synthesis ◽  
Albumin Synthesis ◽  
Rat Hepatocyte ◽  
Monolayer Cultures ◽  
Cryopreservation Method ◽  
Hepatocyte Monolayer

1 Most previous attempts to cryopreserve hepatocytes have used suspensions stored at either - 70°C or in liquid nitrogen, and the major problem is that these do not, on subsequent thawing, attach well in culture. This limits their use in studies of drug metabolism and xenobiotic- induced toxicity. In this manuscript we demonstrate successful cryopreservation of rat hepatocytes as mono layers attached to a collagen film. 2 Monolayers can be frozen and thawed without significant loss of cells, and although damage to the internal and plasma membranes is evident immediately post-thaw, a remarkable repair process takes place over 24-48 h post-thaw. Immediately post-thaw only 10% of the cells exclude Trypan Blue, but by 48 h 80 - 90% of the thawed cells are viable, indicating that repair of the plasma membranes has taken place. 3 The cells post-thaw retain aspects of liver-specific function including cytochrome P450 content and albumin synthesis. However, cytosolic proteins are lost through the damaged membranes and, probably because of this, urea synthesis from ammonia is retained at only 25% of pre- freeze values. 4 A cryopreservation method based on adherent hepa tocytes on a collagen substrate overcomes the problems encountered with culture of cryopreserved hepatocyte suspensions, and may provide a practical means of establishing a 'bank' of hepatocytes from several donors and species.

Regulation of Ketogenesis, Gluconeogenesis, and Glycogen Synthesis by Insulin and Proinsulin in Rat Hepatocyte Monolayer Cultures

Diabetes ◽  
1986 ◽  
Vol 35 (11) ◽  
pp. 1286-1293 ◽  
Author(s):  
L. Agius ◽  
M. H. Chowdhury ◽  
S. N. Davis ◽  
K. G. M. M. Alberti
Keyword(s):  
Glycogen Synthesis ◽  
Rat Hepatocyte ◽  
Monolayer Cultures ◽  
Hepatocyte Monolayer
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