scholarly journals Combined phylogenetic and genomic approaches for the high-throughput study of microbial habitat adaptation

2011 ◽  
Vol 19 (10) ◽  
pp. 472-482 ◽  
Author(s):  
Jesse R.R. Zaneveld ◽  
Laura Wegener Parfrey ◽  
Will Van Treuren ◽  
Catherine Lozupone ◽  
Jose C. Clemente ◽  
...  
Keyword(s):  
High Throughput ◽  
Habitat Adaptation ◽  
Microbial Habitat ◽  
High Throughput Study

scholarly journals Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yang Zhang ◽  
Tuan M. Nguyen ◽  
Xiao-Ou Zhang ◽  
Limei Wang ◽  
Tin Phan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Throughput ◽  
Biological Effect ◽  
Human Hepatocellular Carcinoma ◽  
Guide Rnas ◽  
Rna Targeting ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Screening Library ◽  
High Throughput Study

AbstractShort hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool and optimizing the strategy for designing single-guide RNAs against circRNA BSJ sites, we markedly enhance specificity of circRNA silencing. This specificity is validated in parallel screenings by shRNA and CRISPR/Cas13d libraries. Using a CRISPR/Cas13d screening library targeting > 2500 human hepatocellular carcinoma-related circRNAs, we subsequently identify a subset of sorafenib-resistant circRNAs. Thus, CRISPR/Cas13d represents an effective approach for high-throughput study of functional circRNAs.

A microfluidic platform for the high-throughput study of pathological cardiac hypertrophy

Lab on a Chip ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 3264-3271 ◽  
Author(s):  
Hesam Parsa ◽  
Bryan Z. Wang ◽  
Gordana Vunjak-Novakovic
Keyword(s):  
Cardiac Hypertrophy ◽  
High Throughput ◽  
Volume Overload ◽  
Microfluidic Platform ◽  
Pathological Cardiac Hypertrophy ◽  
High Throughput Study

Currentin vitromodels fall short in deciphering the mechanisms of cardiac hypertrophy induced by volume overload.

A high-throughput study of magnetocaloric materials: Gradient solidification applied to La-Fe-Si

2019 ◽  
Vol 108 ◽  
pp. 100-108 ◽  
Author(s):  
Yi Ouyang ◽  
Mingxiao Zhang ◽  
Jun Li ◽  
Aru Yan ◽  
Jian Liu
Keyword(s):  
High Throughput ◽  
Magnetocaloric Materials ◽  
High Throughput Study

scholarly journals Discovery of (meth)acrylate polymers that resist colonization by fungi associated with pathogenesis and biodeterioration

2020 ◽  
Vol 6 (23) ◽  
pp. eaba6574 ◽  
Author(s):  
Cindy Vallieres ◽  
Andrew L. Hook ◽  
Yinfeng He ◽  
Valentina Cuzzucoli Crucitti ◽  
Grazziela Figueredo ◽  
...  
Keyword(s):  
High Throughput ◽  
Fungal Colonization ◽  
Socioeconomic Impacts ◽  
Killing Effect ◽  
Plant Toxicity ◽  
Leaf Surfaces ◽  
Acrylate Polymers ◽  
Bioactive Agents ◽  
Fungal Deterioration ◽  
High Throughput Study

Fungi have major, negative socioeconomic impacts, but control with bioactive agents is increasingly restricted, while resistance is growing. Here, we describe an alternative fungal control strategy via materials operating passively (i.e., no killing effect). We screened hundreds of (meth)acrylate polymers in high throughput, identifying several that reduce attachment of the human pathogen Candida albicans, the crop pathogen Botrytis cinerea, and other fungi. Specific polymer functional groups were associated with weak attachment. Low fungal colonization materials were not toxic, supporting their passive, anti-attachment utility. We developed a candidate monomer formulation for inkjet-based 3D printing. Printed voice prosthesis components showed up to 100% reduction in C. albicans biofilm versus commercial materials. Furthermore, spray-coated leaf surfaces resisted fungal infection, with no plant toxicity. This is the first high-throughput study of polymer chemistries resisting fungal attachment. These materials are ready for incorporation in products to counteract fungal deterioration of goods, food security, and health.

scholarly journals Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes

2020 ◽  
Vol 5 (4) ◽  
pp. 1062-1070 ◽  
Author(s):  
Yanran Li ◽  
Si Wang ◽  
Yuanjun Dong ◽  
Ping Mu ◽  
Yun Yang ◽  
...  
Keyword(s):  
High Throughput ◽  
Crystalline Phase ◽  
Tio2 Nanotubes ◽  
Cell Behaviors ◽  
High Throughput Study

High-throughput study of martensitic transformations in the complete Ti–Ni–Cu system

2012 ◽  
Vol 26 ◽  
pp. 98-109 ◽  
Author(s):  
Robert Zarnetta ◽  
Pio John S. Buenconsejo ◽  
Alan Savan ◽  
Sigurd Thienhaus ◽  
Alfred Ludwig
Keyword(s):  
High Throughput ◽  
Martensitic Transformations ◽  
High Throughput Study
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