scholarly journals Human DNA Polymerase ι Incorporates dCTP Opposite Template G via a G.C+ Hoogsteen Base Pair

Structure ◽  
2005 ◽  
Vol 13 (10) ◽  
pp. 1569-1577 ◽  
Author(s):  
Deepak T. Nair ◽  
Robert E. Johnson ◽  
Louise Prakash ◽  
Satya Prakash ◽  
Aneel K. Aggarwal
Keyword(s):  
Dna Polymerase ◽  
Base Pair ◽  
Human Dna ◽  
Dna Polymerase Ι

Hoogsteen base pair formation promotes synthesis opposite the 1,N6-ethenodeoxyadenosine lesion by human DNA polymerase ι

2006 ◽  
Vol 13 (7) ◽  
pp. 619-625 ◽  
Author(s):  
Deepak T Nair ◽  
Robert E Johnson ◽  
Louise Prakash ◽  
Satya Prakash ◽  
Aneel K Aggarwal
Keyword(s):  
Dna Polymerase ◽  
Base Pair ◽  
Pair Formation ◽  
Human Dna ◽  
Dna Polymerase Ι

scholarly journals Human DNA Polymerase ι Utilizes Different Nucleotide Incorporation Mechanisms Dependent upon the Template Base

2004 ◽  
Vol 24 (2) ◽  
pp. 936-943 ◽  
Author(s):  
M. Todd Washington ◽  
Robert E. Johnson ◽  
Louise Prakash ◽  
Satya Prakash
Keyword(s):  
Dna Polymerase ◽  
Base Pair ◽  
Translesion Dna Synthesis ◽  
Human Dna ◽  
Nucleotide Incorporation ◽  
Steady State Kinetic ◽  
Y Family ◽  
State Kinetic ◽  
Dna Polymerase Ι ◽  
Better Than

ABSTRACT Human DNA polymerase ι (Polι) is a member of the Y family of DNA polymerases involved in translesion DNA synthesis. Polι is highly unusual in that it possesses a high fidelity on template A, but has an unprecedented low fidelity on template T, preferring to misincorporate a G instead of an A. To understand the mechanisms of nucleotide incorporation opposite different template bases by Polι, we have carried out pre-steady-state kinetic analyses of nucleotide incorporation opposite templates A and T. These analyses have revealed that opposite template A, the correct nucleotide is preferred because it is bound tighter and is incorporated faster than the incorrect nucleotides. Opposite template T, however, the correct and incorrect nucleotides are incorporated at very similar rates, and interestingly, the greater efficiency of G misincorporation relative to A incorporation opposite T arises predominantly from the tighter binding of G. Based on these results, we propose that the incipient base pair is accommodated differently in the active site of Polι dependent upon the template base and that when T is the templating base, Polι accommodates the wobble base pair better than the Watson-Crick base pair.

Response of human DNA polymerase ι promoter to N-methyl-N′-nitro-N-nitrosoguanidine

2010 ◽  
Vol 29 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Huifang Zhu ◽  
Yanfeng Fan ◽  
Hongjuan Jiang ◽  
Jing Shen ◽  
Hongyan Qi ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Human Dna ◽  
Dna Polymerase Ι

Replication by human DNA polymerase-ι occurs by Hoogsteen base-pairing

Nature ◽  
2004 ◽  
Vol 430 (6997) ◽  
pp. 377-380 ◽  
Author(s):  
Deepak T. Nair ◽  
Robert E. Johnson ◽  
Satya Prakash ◽  
Louise Prakash ◽  
Aneel K. Aggarwal
Keyword(s):  
Dna Polymerase ◽  
Base Pairing ◽  
Human Dna ◽  
Hoogsteen Base Pairing ◽  
Dna Polymerase Ι

scholarly journals Human DNA Polymerase ι Promotes Replication through a Ring-Closed Minor-Groove Adduct That Adopts a syn Conformation in DNA

2005 ◽  
Vol 25 (19) ◽  
pp. 8748-8754 ◽  
Author(s):  
William T. Wolfle ◽  
Robert E. Johnson ◽  
Irina G. Minko ◽  
R. Stephen Lloyd ◽  
Satya Prakash ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
Minor Groove ◽  
Dna Lesions ◽  
Unsaturated Aldehyde ◽  
Cyclic Form ◽  
Human Dna ◽  
A Minor ◽  
Dna Polymerase Ι

ABSTRACT Acrolein, an α,β-unsaturated aldehyde, is generated in vivo as the end product of lipid peroxidation and from oxidation of polyamines. The reaction of acrolein with the N 2 group of guanine in DNA leads to the formation of a cyclic adduct, γ-hydroxy-1,N 2-propano-2′-deoxyguanosine (γ-HOPdG). Previously, we have shown that proficient replication through the γ-HOPdG adduct can be mediated by the sequential action of human DNA polymerases (Pols) ι and κ, in which Polι incorporates either pyrimidine opposite γ-HOPdG, but Polκ extends only from the cytosine. Since γ-HOPdG can adopt either a ring-closed cyclic form or a ring-opened form in DNA, to better understand the mechanisms that Pols ι and κ employ to promote replication through this lesion, we have examined the ability of these polymerases to replicate through the structural analogs of γ-HOPdG that are permanently either ring closed or ring opened. Our studies with these model adducts show that whereas the ring-opened form of γ-HOPdG is not inhibitory to synthesis by human Pols η, ι, or κ, only Polι is able to incorporate nucleotides opposite the ring-closed form, which is known to adopt a syn conformation in DNA. From these studies, we infer that (i) Pols η, ι, and κ have the ability to proficiently replicate through minor-groove DNA lesions that do not perturb the Watson-Crick hydrogen bonding of the template base with the incoming nucleotide, and (ii) Polι can accommodate a minor-groove-adducted template purine which adopts a syn conformation in DNA and forms a Hoogsteen base pair with the incoming nucleotide.

scholarly journals Lesion Bypass ofN2-Ethylguanine by Human DNA Polymerase ι

2008 ◽  
Vol 284 (3) ◽  
pp. 1732-1740 ◽  
Author(s):  
Matthew G. Pence ◽  
Patrick Blans ◽  
Charles N. Zink ◽  
Thomas Hollis ◽  
James C. Fishbein ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Lesion Bypass ◽  
Human Dna ◽  
Dna Polymerase Ι

scholarly journals Structural Basis for Proficient Incorporation of dTTP OppositeO6-Methylguanine by Human DNA Polymerase ι

2010 ◽  
Vol 285 (52) ◽  
pp. 40666-40672 ◽  
Author(s):  
Matthew G. Pence ◽  
Jeong-Yun Choi ◽  
Martin Egli ◽  
F. Peter Guengerich
Keyword(s):  
Dna Polymerase ◽  
Structural Basis ◽  
Human Dna ◽  
Dna Polymerase Ι

scholarly journals Proliferating Cell Nuclear Antigen-dependent Coordination of the Biological Functions of Human DNA Polymerase ι

2004 ◽  
Vol 279 (46) ◽  
pp. 48360-48368 ◽  
Author(s):  
Antonio E. Vidal ◽  
Patricia Kannouche ◽  
Vladimir N. Podust ◽  
Wei Yang ◽  
Alan R. Lehmann ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Biological Functions ◽  
Human Dna ◽  
Proliferating Cell ◽  
Dna Polymerase Ι
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