Repeat estradiol exposure differentially regulates protein expression patterns for estrogen receptor and E-cadherin in older mouse ovarian surface epithelium: Implications for replacement and adjuvant hormone therapies?

Steroids ◽  
2012 ◽  
Vol 77 (6) ◽  
pp. 674-685 ◽  
Author(s):  
Linda S.M. Gulliver ◽  
Peter R. Hurst
Keyword(s):  
Estrogen Receptor ◽  
Protein Expression ◽  
Expression Patterns ◽  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Ovarian Surface ◽  
Hormone Therapies ◽  
E Cadherin

scholarly journals E-cadherin induces mesenchymal-to-epithelial transition in human ovarian surface epithelium

1999 ◽  
Vol 96 (11) ◽  
pp. 6249-6254 ◽  
Author(s):  
N. Auersperg ◽  
J. Pan ◽  
B. D. Grove ◽  
T. Peterson ◽  
J. Fisher ◽  
...  
Keyword(s):  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Ovarian Surface ◽  
Mesenchymal To Epithelial Transition ◽  
E Cadherin

scholarly journals Phenotypic Plasticity of the Ovarian Surface Epithelium: TGF-β1 Induction of Epithelial to Mesenchymal Transition (EMT) in Vitro

Endocrinology ◽  
2010 ◽  
Vol 151 (11) ◽  
pp. 5497-5505 ◽  
Author(s):  
Yihong Zhu ◽  
Mikael Nilsson ◽  
Karin Sundfeldt
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Mesenchymal Transition ◽  
Epithelial Markers ◽  
Ovarian Surface ◽  
Epithelial Junction ◽  
Tgf Β1 ◽  
E Cadherin

Ovarian surface epithelium (OSE) is the most conceivable cell origin of epithelial ovarian carcinomas. Unlike many other epithelial tumors, the precancerous lesion acquires expression of epithelial markers, e.g. E-cadherin and claudins, suggesting that OSE cells undergo mesenchymal to epithelial transition (MET) during transformation. Recent findings indicate that TGF-β1, a prototypic stimulus of epithelial to mesenchymal transition (EMT), i.e. reverse to MET, is produced at significant amounts in the intact ovary. In the present study, we therefore investigated whether TGF-β1 changes the OSE phenotype accordingly, focusing on epithelial junction proteins and transcriptional EMT regulators quantified by real-time RT-PCR and Western blotting in cultured normal human OSE. Early OSE passages were found to paradoxically express de novo E-cadherin and also establish tight junctions exhibiting claudin-1 (but not claudin-3 and -4) and occludin. Stimulation with TGF-β1 (100 ng/ml) for 3–5 d down-regulated all these epithelial markers including Crumbs3 and also prevented the formation of an epithelial barrier This was accompanied by sustained expression of Snail and N-cadherin and transient expression of Slug, whereas Zeb1 (zinc finger E-box binding homeobox 1) and Twist mRNA levels were not significantly changed. In conclusion, TGF-β1 enforces the mesenchymal phenotype of OSE cells in vitro by an EMT-like process, leading to an altered molecular composition of the epithelial junction complex that partly coincides with the expression pattern of the native OSE. This suggests a potential role of TGF-β1-induced EMT in OSE under physiological conditions and possibly also in epithelial ovarian tumorigenesis.

scholarly journals Autoradiographic investigation of estrogen binding in cultured rat ovarian surface epithelial cells.

1983 ◽  
Vol 31 (11) ◽  
pp. 1321-1325 ◽  
Author(s):  
A T Adams ◽  
N Auersperg
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptors ◽  
Cultured Cells ◽  
Ovarian Surface Epithelium ◽  
Live Cells ◽  
Surface Epithelium ◽  
Target Tissue ◽  
Ovarian Carcinomas ◽  
Ovarian Surface ◽  
Freeze Dried

Autoradiographic evidence presented that the cultured rat ovarian surface epithelium exhibits estrogen receptor-like activity. Two autoradiographic techniques were used; one involved live cells that were labeled and freeze-dried, and the other the labeling of ethanol-fixed cells. Autoradiograms were prepared by dipping cells grown on plastic cover slips in liquid nuclear track emulsion. Exposure times were 1 to 4 weeks. Experiments using a pulse-chase technique in live cells and steroid competition tests in fixed and live cells gave evidence for translocation of tritiated estradiol from cytoplasm to nucleus in live cells and for a component of estrogen-specific binding in live and fixed cells. The techniques presented here for the investigation of estrogen receptors in cultured cells require little tissue, are simple, and relatively quick. Reports based on biochemical analyses of tissue homogenates claim the presence of estrogen receptors in human ovarian carcinomas of surface epithelial origin and in rat ovarian surface epithelium. The results of this study add further evidence that the ovarian surface epithelium has estrogen receptor activity and should be considered an estrogen target tissue.

scholarly journals Induction of Proliferation in the Primate Ovarian Surface Epithelium In Vivo

2008 ◽  
Vol 63 (5) ◽  
pp. 307-308
Author(s):  
Jay W. Wright ◽  
Tanja Pejovic ◽  
John Fanton ◽  
Richard L. Stouffer
Keyword(s):  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Ovarian Surface
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