scholarly journals Nodal/Activin Signaling Predicts Human Pluripotent Stem Cell Lines Prone to Differentiate Toward the Hematopoietic Lineage

2010 ◽  
Vol 18 (12) ◽  
pp. 2173-2181 ◽  
Author(s):  
Veronica Ramos-Mejia ◽  
Gustavo J Melen ◽  
Laura Sanchez ◽  
Ivan Gutierrez-Aranda ◽  
Gertrudis Ligero ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Activin Signaling ◽  
Hematopoietic Lineage ◽  
Stem Cell Lines

scholarly journals Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs

2016 ◽  
Vol 16 (3) ◽  
pp. 735-739
Author(s):  
Federico González-Pozas ◽  
Rosa Montes ◽  
Lourdes López-Onieva ◽  
Tamara Romero ◽  
Joan Domingo-Reinés ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Notch Ligand ◽  
Short Hairpin ◽  
Stem Cell Lines ◽  
Short Hairpin Rnas

Hypoxic culture of human pluripotent stem cell lines is permissible using mouse embryonic fibroblasts

2012 ◽  
Vol 7 (5) ◽  
pp. 675-683 ◽  
Author(s):  
Jennifer L Badger ◽  
Meg L Byrne ◽  
Farlan S Veraitch ◽  
Chris Mason ◽  
Ivan B Wall ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Stem Cell Lines

scholarly journals Generation of human pluripotent stem cell lines (iPSCs) from mesenchymal stem cells (MSCs) from three elderly patients with osteoarthritis

2020 ◽  
Vol 44 ◽  
pp. 101721 ◽  
Author(s):  
Lydiane Pichard ◽  
Jean-Marc Brondelo ◽  
Fabienne Becker ◽  
Romain Desprat ◽  
Frédéric De Ceuninck ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Elderly Patients ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Lines

scholarly journals Expression of miRNAs from the Imprinted DLK1/DIO3 Locus Signals the Osteogenic Potential of Human Pluripotent Stem Cells

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1523 ◽  
Author(s):  
Laetitia Barrault ◽  
Jacqueline Gide ◽  
Tingting Qing ◽  
Lea Lesueur ◽  
Jorg Tost ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Osteogenic Potential ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Lines

Substantial variations in differentiation properties have been reported among human pluripotent cell lines (hPSC), which could affect their utility and clinical safety. We characterized the variable osteogenic capacity observed between different human pluripotent stem cell lines. By focusing on the miRNA expression profile, we demonstrated that the osteogenic differentiation propensity of human pluripotent stem cell lines could be associated with the methylation status and the expression of miRNAs from the imprinted DLK1/DIO3 locus. More specifically, quantitative analysis of the expression of six different miRNAs of that locus prospectively identified human embryonic stem cells and human-induced pluripotent stem cells with differential osteogenic differentiation capacities. At the molecular and functional levels, we showed that these miRNAs modulated the expression of the activin receptor type 2B and the downstream signal transduction, which impacted osteogenesis. In conclusion, miRNAs of the imprinted DLK1/DIO3 locus appear to have both a predictive value and a functional impact in determining the osteogenic fate of human pluripotent stem cells.

scholarly journals Establishment of a collection of human pluripotent stem cell lines (iPSC) from mesenchymal stem cells (MSC) from three healthy elderly donors

2021 ◽  
Vol 53 ◽  
pp. 102297
Author(s):  
Lydiane Pichard ◽  
Jean-Marc Brondello ◽  
Fabienne Becker ◽  
Romain Desprat ◽  
Frédéric De Ceuninck ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Healthy Elderly ◽  
Stem Cell Lines

scholarly journals Cost-Effective Master Cell Bank Validation of Multiple Clinical-Grade Human Pluripotent Stem Cell Lines From a Single Donor

2014 ◽  
Vol 3 (10) ◽  
pp. 1116-1124 ◽  
Author(s):  
Liani Devito ◽  
Anastasia Petrova ◽  
Cristian Miere ◽  
Stefano Codognotto ◽  
Nicola Blakely ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Clinical Grade ◽  
Human Pluripotent Stem Cell ◽  
Cell Bank ◽  
Single Donor ◽  
Master Cell Bank ◽  
Stem Cell Lines
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