scholarly journals Muscle Stem Cells Undergo Extensive Clonal Drift during Tissue Growth via Meox1-Mediated Induction of G2 Cell-Cycle Arrest

2017 ◽  
Vol 21 (1) ◽  
pp. 107-119.e6 ◽  
Author(s):  
Phong Dang Nguyen ◽  
David Baruch Gurevich ◽  
Carmen Sonntag ◽  
Lucy Hersey ◽  
Sara Alaei ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Cycle Arrest ◽  
Tissue Growth ◽  
Muscle Stem Cells ◽  
Cycle Arrest ◽  
G2 Cell Cycle Arrest

scholarly journals PAWI-2 overcomes tumor stemness and drug resistance via cell cycle arrest in integrin β3-KRAS-dependent pancreatic cancer stem cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiongjia Cheng ◽  
John R. Cashman
Keyword(s):  
Cell Cycle ◽  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Cycle Arrest ◽  
Specific Inhibitor ◽  
Feedback Mechanism ◽  
Major Health Problem ◽  
Cycle Arrest ◽  
Drug Inhibition

Abstract Today, pancreatic cancer (PC) remains a major health problem in the US. The fact that cancer stem cells (CSCs) become enriched in humans following anti-cancer therapy implicates CSCs as key contributors to tumor dormancy, metastasis, and relapse in PC. A highly validated CSC model (FGβ3 cells) was used to test a novel compound (PAWI-2) to eradicate CSCs. Compared to parental bulk FG cells, PAWI-2 showed greater potency to inhibit cell viability and self-renewal capacity of FGβ3 cells. For FGβ3 cells, dysregulated integrin β3-KRAS signaling drives tumor progression. PAWI-2 inhibited β3-KRAS signaling independent of KRAS. This is clinically relevant. PAWI-2 targeted the downstream TBK1 phosphorylation cascade that was negatively regulated by optineurin phosphorylation via a feedback mechanism. This was confirmed by TBK1 genetic knockdown or co-treatment with TBK1-specific inhibitor (MRT67307). PAWI-2 also overcame erlotinib (an EGFR inhibitor) resistance in FGβ3 cells more potently than bortezomib. In the proposed working model, optineurin acts as a key regulator to link inhibition of KRAS signaling and cell cycle arrest (G2/M). The findings show PAWI-2 is a new approach to reverse tumor stemness that resensitizes CSC tumors to drug inhibition.

scholarly journals G1 versus G2 cell cycle arrest after adriamycin-induced damage in mouse Swiss3T3 cells

FEBS Letters ◽  
1999 ◽  
Vol 461 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Wai Yi Siu ◽  
Cain H Yam ◽  
Randy Y.C Poon
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cycle Arrest ◽  
G2 Cell Cycle Arrest

scholarly journals Critical role of PP2A-B56 family protein degradation in HIV-1 Vif mediated G2 cell cycle arrest

2020 ◽  
Vol 527 (1) ◽  
pp. 257-263
Author(s):  
Kayoko Nagata ◽  
Keisuke Shindo ◽  
Yusuke Matsui ◽  
Kotaro Shirakawa ◽  
Akifumi Takaori-Kondo
Keyword(s):  
Cell Cycle ◽  
Protein Degradation ◽  
Cell Cycle Arrest ◽  
Critical Role ◽  
Cycle Arrest ◽  
Family Protein ◽  
G2 Cell Cycle Arrest ◽  
Hiv 1

scholarly journals Weighted Correlation Network Analysis Reveals CDK2 as a Regulator of a Ubiquitous Environmental Toxin-Induced Cell-Cycle Arrest

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 143
Author(s):  
Virginie Dubourg ◽  
Alexander Nolze ◽  
Michael Kopf ◽  
Michael Gekle ◽  
Gerald Schwerdt
Keyword(s):  
Cell Cycle ◽  
Network Analysis ◽  
Cell Cycle Arrest ◽  
Regulation Of Gene Expression ◽  
Correlation Network ◽  
Food Contaminants ◽  
Proximal Tubule Cells ◽  
Cycle Arrest ◽  
G2 Cell Cycle Arrest ◽  
Weighted Correlation

Environmental food contaminants constitute a threat to human health. For instance, the globally spread mycotoxin Ochratoxin A (OTA) contributes to chronic kidney damage by affecting proximal tubule cells via unknown mechanisms. We applied a top-down approach to identify relevant toxicological mechanisms of OTA using RNA-sequencing followed by in-depth bioinformatics analysis and experimental validation. Differential expression analyses revealed that OTA led to the regulation of gene expression in kidney human cell lines, including for genes enriched in cell cycle-related pathways, and OTA-induced gap 1 and 2 (G1 and G2) cell-cycle arrests were observed. Weighted correlation network analysis highlighted cyclin dependent kinase 2 (CDK2) as a putative key regulator of this effect. CDK2 was downregulated by OTA exposure, and its overexpression partially blocked the OTA-induced G1 but not G2 cell-cycle arrest. We, therefore, propose CDK2 as one of the key regulators of the G1 cell-cycle arrest induced by low nanomolar concentrations of OTA.

scholarly journals Sphingosine kinases protect murine embryonic stem cells from sphingosine-induced cell cycle arrest

Stem Cells ◽  
2020 ◽  
Vol 38 (5) ◽  
pp. 613-623
Author(s):  
Suveg Pandey ◽  
Kelly M. Banks ◽  
Ritu Kumar ◽  
Andrew Kuo ◽  
Duancheng Wen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Cycle Arrest ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Cycle Arrest ◽  
Sphingosine Kinases
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