scholarly journals Stem Cells Go Soft: Pliant Substrate Surfaces Enhance Motor Neuron Differentiation

2014 ◽  
Vol 14 (6) ◽  
pp. 701-703 ◽  
Author(s):  
Ning Wang
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Neuron Differentiation ◽  
Substrate Surfaces

Microtubule stabilizer epothilone B as a motor neuron differentiation agent for human endometrial stem cells

2020 ◽  
Vol 44 (5) ◽  
pp. 1168-1183 ◽  
Author(s):  
Narges Mahmoodi ◽  
Jafar Ai ◽  
Somayeh Ebrahimi‐Barough ◽  
Zahra Hassannejad ◽  
Elham Hasanzadeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Neuron Differentiation ◽  
Epothilone B ◽  
Endometrial Stem Cells ◽  
Microtubule Stabilizer

Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers

2016 ◽  
Vol 4 (19) ◽  
pp. 3305-3312 ◽  
Author(s):  
Yadong Tang ◽  
Li Liu ◽  
Junjun Li ◽  
Leqian Yu ◽  
Francesco Paolo Ulloa Severino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Electrode Array ◽  
Plug And Play ◽  
Neuron Differentiation ◽  
Neuron Spike ◽  
Induced Pluripotent ◽  
Multi Electrode Array

A patch made of crosslinked monolayer nanofibers was used for motor neuron differentiation from human induced pluripotent stem cells and plug-and-play with a commercial multi-electrode array for neuron spike recording.

Purmorphamine as a Shh Signaling Activator Small Molecule Promotes Motor Neuron Differentiation of Mesenchymal Stem Cells Cultured on Nanofibrous PCL Scaffold

2016 ◽  
Vol 54 (7) ◽  
pp. 5668-5675 ◽  
Author(s):  
Naghmeh Bahrami ◽  
Mohammad Bayat ◽  
Abdolreza Mohamadnia ◽  
Mehrdad Khakbiz ◽  
Meysam Yazdankhah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Small Molecule ◽  
Shh Signaling ◽  
Neuron Differentiation ◽  
Cells Cultured

scholarly journals Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daisuke Shimojo ◽  
Kazunari Onodera ◽  
Yukiko Doi-Torii ◽  
Yasuharu Ishihara ◽  
Chinatsu Hattori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Simple Motor

scholarly journals High-efficiency motor neuron differentiation from human pluripotent stem cells and the function of Islet-1

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Qiuhao Qu ◽  
Dong Li ◽  
Kathleen R. Louis ◽  
Xiangzhen Li ◽  
Hong Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Islet 1

scholarly journals Notch Signaling Regulates Motor Neuron Differentiation of Human Embryonic Stem Cells

Stem Cells ◽  
2015 ◽  
Vol 33 (2) ◽  
pp. 403-415 ◽  
Author(s):  
Etti Ben-Shushan ◽  
Eva Feldman ◽  
Benjamin E. Reubinoff
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Motor Neuron ◽  
Notch Signaling ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neuron Differentiation

scholarly journals The Ubiquitin Proteasome System Is a Key Regulator of Pluripotent Stem Cell Survival and Motor Neuron Differentiation

Cells ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 581 ◽  
Author(s):  
Monique Bax ◽  
Jessie McKenna ◽  
Dzung Do-Ha ◽  
Claire H. Stevens ◽  
Sarah Higginbottom ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Ubiquitin Proteasome System ◽  
Neuron Differentiation ◽  
Stem Cell Survival ◽  
Ubiquitin Proteasome

The ubiquitin proteasome system (UPS) plays an important role in regulating numerous cellular processes, and a dysfunctional UPS is thought to contribute to motor neuron disease. Consequently, we sought to map the changing ubiquitome in human iPSCs during their pluripotent stage and following differentiation to motor neurons. Ubiquitinomics analysis identified that spliceosomal and ribosomal proteins were more ubiquitylated in pluripotent stem cells, whilst proteins involved in fatty acid metabolism and the cytoskeleton were specifically ubiquitylated in the motor neurons. The UPS regulator, ubiquitin-like modifier activating enzyme 1 (UBA1), was increased 36-fold in the ubiquitome of motor neurons compared to pluripotent stem cells. Thus, we further investigated the functional consequences of inhibiting the UPS and UBA1 on motor neurons. The proteasome inhibitor MG132, or the UBA1-specific inhibitor PYR41, significantly decreased the viability of motor neurons. Consistent with a role of the UPS in maintaining the cytoskeleton and regulating motor neuron differentiation, UBA1 inhibition also reduced neurite length. Pluripotent stem cells were extremely sensitive to MG132, showing toxicity at nanomolar concentrations. The motor neurons were more resilient to MG132 than pluripotent stem cells but demonstrated higher sensitivity than fibroblasts. Together, this data highlights the important regulatory role of the UPS in pluripotent stem cell survival and motor neuron differentiation.

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