scholarly journals Hair Follicle Epithelial Stem Cells Get Their Sox On

2008 ◽  
Vol 3 (1) ◽  
pp. 3-4 ◽  
Author(s):  
Angela M. Christiano
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Epithelial Stem Cells

scholarly journals Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures

2018 ◽  
Vol 2018 ◽  
pp. 1-14
Author(s):  
Jun Liu ◽  
Claire A. Higgins ◽  
Jenna C. Whitehouse ◽  
Susan J. Harris ◽  
Heather Crawford ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Hair Follicle ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
Dermal Papilla ◽  
Epithelial Stem Cells ◽  
Stem Cell Maintenance ◽  
Induced Pluripotent ◽  
Dermal Cells

In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within folliclesin situin a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.

scholarly journals Keratin 15 Promoter Targets Putative Epithelial Stem Cells in the Hair Follicle Bulge

2003 ◽  
Vol 121 (5) ◽  
pp. 963-968 ◽  
Author(s):  
Yaping Liu ◽  
Stephen Lyle ◽  
Zaixin Yang ◽  
George Cotsarelis
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Epithelial Stem Cells

scholarly journals Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Stéphanie Claudinot ◽  
Jun-Ichi Sakabe ◽  
Hideo Oshima ◽  
Christèle Gonneau ◽  
Thimios Mitsiadis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Epidermal Cells ◽  
Epithelial Stem Cells ◽  
Sebaceous Glands ◽  
Embryonic Origin ◽  
Lineage Markers

Abstract The formation of hair follicles, a landmark of mammals, requires complex mesenchymal–epithelial interactions and it is commonly believed that embryonic epidermal cells are the only cells that can respond to hair follicle morphogenetic signals in vivo. Here, we demonstrate that epithelial stem cells of non-skin origin (e.g. that of cornea, oesophagus, vagina, bladder, prostate) that express the transcription factor Tp63, a master gene for the development of epidermis and its appendages, can respond to skin morphogenetic signals. When exposed to a newborn skin microenvironment, these cells express hair-follicle lineage markers and contribute to hair follicles, sebaceous glands and/or epidermis renewal. Our results demonstrate that lineage restriction is not immutable and support the notion that all Tp63-expressing epithelial stem cells, independently of their embryonic origin, have latent skin competence explaining why aberrant hair follicles or sebaceous glands are sometimes observed in non-skin tissues (e.g. in cornea, vagina or thymus).

Dermal papilla-induced hair differentiation of adult epithelial stem cells from human skin

2004 ◽  
Vol 19 (2) ◽  
pp. 207-217 ◽  
Author(s):  
Cecilia Roh ◽  
Qingfeng Tao ◽  
Stephen Lyle
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Cell Fate ◽  
Time Course ◽  
Dermal Papilla ◽  
Fold Increase ◽  
Keratinocyte Differentiation ◽  
Epithelial Stem Cells ◽  
High Calcium ◽  
Keratinocyte Stem Cells

The epithelial-mesenchymal interactions between keratinocyte stem cells and dermal papilla (DP) cells are crucial for normal development of the hair follicle as well as during hair cycling. During the cyclical regrowth of a new lower follicle, the multipotent hair follicle stem cells are stimulated to proliferate and differentiate through interactions with the underlying mesenchymal DP cells. To characterize the events occurring during the process of epithelial stem cell fate determination, we utilized a coculture system by incubating human hair follicle keratinocyte stem cells with DP cells. Using GeneChip microarrays, we analyzed changes in gene expression within the stem cells upon coculture with the DP over a 5-day time course. A number of important signaling pathways and growth factors were regulated. The hair-specific keratin 6hf (K6hf) gene proved a particularly good marker of hair differentiation, with a 7.9-fold increase in mRNA and resulting increased protein levels. The high expression of K6hf was unique to DP-induced keratinocyte differentiation, since expression of K6hf was not induced by high calcium. Since the β-catenin signaling pathway has been implicated in hair follicle development, we examined the role of β-catenin in our system and demonstrated that β-catenin/lef-1 signaling is required for DP-induced hair differentiation.

scholarly journals 621 An apple stem cell-derived extract increases epithelial stem cells number in the hair follicle bulge

2019 ◽  
Vol 139 (9) ◽  
pp. S321
Author(s):  
M. Fehrholz ◽  
F. Wandrey ◽  
I. Piccini ◽  
J. Gherardini ◽  
M.A. Alam ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hair Follicle ◽  
Epithelial Stem Cells ◽  
Apple Stem

A simple culture method for epithelial stem cells derived from human hair follicle

2013 ◽  
Vol 8 (5) ◽  
pp. 432-439 ◽  
Author(s):  
Abu Hilmi ◽  
Ahmad Halim ◽  
Norhayati Noor ◽  
Chin Lim ◽  
Zamzuri Idris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Human Hair ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Epidermal Layer ◽  
Epithelial Stem Cells ◽  
Human Hair Follicle ◽  
Color Flow ◽  
Defined Culture

AbstractThe challenge arises among researchers when hair follicle stem cells (HFSCs) derived from a human hair follicle remain poorly expanded in defined culture medium. In this study, we isolated the HFSCs and they became confluent after 10 days of cultivation. Comparing the viability of HFSCs cultured in defined keratinocytes serum free medium (KSFM) in a coated plate and CnT07 medium in an uncoated plate, the number of HFSCs cultured in CnT07 was significantly higher at days 2, 4, 6 and 8 (P=0.004). The population doubling time of HFSCs was 21.48±0.44 hours in non-coated plates with CnT07 and 30.73±0.75 hours in coated plates with KSFM. Our primary HFSC cultures were positive for CD200 and K15 with brownish color. Flow cytometry analysis showed that the percentage of HFSCs expressing CD200 and K15 were 65.20±3.16 and 72.07±6.62 respectively. After reaching 100% confluence, the HFSCs were differentiated into an epidermal layer in vitro using CnT02-3D defined media. HFSCs were differentiated into an epidermal layer after 2 weeks of induction. Involucrin- and K6-positive cells were detected in the differentiated epidermal layer. This method is a simple technique for HFSC isolation and has a lower cost of processing and labor, and it represents a promising tool for skin tissue engineering.

scholarly journals Skin stem cells: rising to the surface

2008 ◽  
Vol 180 (2) ◽  
pp. 273-284 ◽  
Author(s):  
Elaine Fuchs
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Sebaceous Gland ◽  
Wound Repair ◽  
Tissue Homeostasis ◽  
Epithelial Stem Cells ◽  
The Past ◽  
Protective Barrier ◽  
Mammalian Skin ◽  
Skin Epidermis

The skin epidermis and its appendages provide a protective barrier that is impermeable to harmful microbes and also prevents dehydration. To perform their functions while being confronted with the physicochemical traumas of the environment, these tissues undergo continual rejuvenation through homeostasis, and, in addition, they must be primed to undergo wound repair in response to injury. The skin's elixir for maintaining tissue homeostasis, regenerating hair, and repairing the epidermis after injury is its stem cells, which reside in the adult hair follicle, sebaceous gland, and epidermis. Stem cells have the remarkable capacity to both self-perpetuate and also give rise to the differentiating cells that constitute one or more tissues. In recent years, scientists have begun to uncover the properties of skin stem cells and unravel the mysteries underlying their remarkable capacity to perform these feats. In this paper, I outline the basic lineages of the skin epithelia and review some of the major findings about mammalian skin epithelial stem cells that have emerged in the past five years.

The C8/144B monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells

1998 ◽  
Vol 111 (21) ◽  
pp. 3179-3188 ◽  
Author(s):  
S. Lyle ◽  
M. Christofidou-Solomidou ◽  
Y. Liu ◽  
D.E. Elder ◽  
S. Albelda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Monoclonal Antibody ◽  
Hair Follicle ◽  
Human Hair ◽  
Expression Cloning ◽  
Cell Phenotype ◽  
Epithelial Stem Cells ◽  
Human Hair Follicle ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Stem cells are vital for the homeostasis of self-renewing tissues such as the hair follicle. Epithelial stem cells have been implicated in tumorigenesis and wound healing, and their manipulation may have wide ranging applications including gene therapy and tissue transplantation. Rodent hair follicle stem cells have been localized to an area of the follicle called the bulge, however, the identification and characterization of human hair follicle stem cells has been hampered by a lack of cellular markers for this area. We have determined that the C8/144B monoclonal antibody, originally generated against a short intracytoplasmic peptide of CD8, preferentially immunostains hair follicle bulge keratinocytes without staining the remaining hair follicle. Using expression cloning, we identified cytokeratin 15 as the keratinocyte protein recognized by the C8/144B monoclonal antibody. By delineating the bulge using this antibody, we demonstrated that bulge cells possess a stem cell phenotype characterized by their slowly-cycling nature, preferential proliferation at the onset of new hair follicle growth, high level of beta1 integrin expression, and expression of cytokeratin 19.

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