Quantitative detection of multiplex cardiac biomarkers with encoded SERS nanotags on a single T line in lateral flow assay

2018 ◽  
Vol 277 ◽  
pp. 502-509 ◽  
Author(s):  
Di Zhang ◽  
Li Huang ◽  
Bing Liu ◽  
Enben Su ◽  
Hong-Yuan Chen ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Cardiac Biomarkers ◽  
Quantitative Detection

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of aflatoxin B1 in crops

Talanta ◽  
2016 ◽  
Vol 161 ◽  
pp. 297-303 ◽  
Author(s):  
Yong Zhao ◽  
Xiao Liu ◽  
Xiaochen Wang ◽  
Chongyun Sun ◽  
Xinrui Wang ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection

Sample Preincubation Strategy for Sensitive and Quantitative Detection of Clenbuterol in Swine Urine Using a Fluorescent Microsphere–Based Immunochromatographic Assay

2014 ◽  
Vol 77 (11) ◽  
pp. 1998-2003 ◽  
Author(s):  
SHENG L. DENG ◽  
SHAN SHAN ◽  
CHAO L. XU ◽  
DAO F. LIU ◽  
YONG H. XIONG ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Rapid Screening ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescent Microsphere ◽  
Swine Urine ◽  
Relative Standard

We describe an ultrasensitive and quantitative immunochromatographic assay to determine the amount of clenbuterol (CLB) in swine urine. In this study, fluorescein isothiocyanate polystyrene fluorescent microspheres were used as probes. A sample preincubation strategy was introduced to this immunochromatographic assay. Results showed that the strategy evidently improved the sensitivity and accuracy of lateral flow assay. The method was completed in 20 min, and a half-maximal inhibitory concentration of 0.13 μg liter−1 was obtained. The limit of detection of the proposed method to determine CLB in swine urine was 0.01 μg liter−1, which was lower than the limit of detection of immunochromatographic assays without preincubation. Intra-and interday recoveries of spiked swine urine ranged from 85.0 to 107.5%. The relative standard deviation values of the preincubated test strip ranged from 2.7 to 12.5%. Analysis of the CLB in swine urine samples showed that the result obtained from the lateral flow assay is consistent with that obtained from a commercial enzyme-linked immunosorbent assay kit. Our results suggest that the developed fluorescent microsphere–based immunochromatographic assay may be useful as a rapid screening method to detect CLB quantitatively.

An up-converting phosphor technology-based lateral flow assay for point-of-collection detection of morphine and methamphetamine in saliva

The Analyst ◽  
2018 ◽  
Vol 143 (19) ◽  
pp. 4646-4654 ◽  
Author(s):  
Qiushi Hu ◽  
Qiaozhen Wei ◽  
Pingping Zhang ◽  
Shuang Li ◽  
Lei Xue ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection

Rapid and quantitative detection of morphine and methamphetamine in saliva with high sensitivity and accuracy by an UPT-LFA.

Electrochemical studies of lateral flow assay test results for procalcitonin detection

2021 ◽  
Author(s):  
Yachana Gupta Gupta ◽  
Kalpana ◽  
Aditya Sharma Ghrera
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Qualitative Analysis ◽  
Test Line ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection ◽  
Cyclic Voltammograms ◽  
Paper Strip ◽  
Electrochemical Studies

In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml.

Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

2009 ◽  
Vol 79 (1) ◽  
pp. 121-123 ◽  
Author(s):  
Qing Qu ◽  
Ziwen Zhu ◽  
Yufei Wang ◽  
Zhijun Zhong ◽  
Jin Zhao ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection

SERS-based lateral flow assay for quantitative detection of C-reactive protein as an early bio-indicator of a radiation-induced inflammatory response in nonhuman primates

The Analyst ◽  
2018 ◽  
Vol 143 (9) ◽  
pp. 2115-2121 ◽  
Author(s):  
Zhen Rong ◽  
Rui Xiao ◽  
Shuang Xing ◽  
Guolin Xiong ◽  
Zuyin Yu ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nonhuman Primates ◽  
Field Evaluation ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection ◽  
C Reactive Protein ◽  
Acute Radiation Syndrome ◽  
Reactive Protein ◽  
Radiation Induced

We demonstrate a SERS-based lateral flow assay for rapid and quantitative detection of CRP and its potential for in-field evaluation of acute radiation syndrome.

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