Label-free fluorescent assay for high sensitivity and selectivity detection of acid phosphatase and inhibitor screening

2016 ◽  
Vol 234 ◽  
pp. 470-477 ◽  
Author(s):  
Jing Wang ◽  
Yu Yan ◽  
Xu Yan ◽  
Tianyu Hu ◽  
Xiaojian Tang ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
High Sensitivity ◽  
Label Free ◽  
Fluorescent Assay ◽  
Inhibitor Screening ◽  
Sensitivity And Selectivity

A colorimetric and near-infrared fluorescent probe with high sensitivity and selectivity for acid phosphatase and inhibitor screening

2014 ◽  
Vol 50 (63) ◽  
pp. 8677 ◽  
Author(s):  
Yongqian Xu ◽  
Benhao Li ◽  
Liangliang Xiao ◽  
Jia Ouyang ◽  
Shiguo Sun ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
High Sensitivity ◽  
Inhibitor Screening ◽  
Sensitivity And Selectivity

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

scholarly journals A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

RSC Advances ◽  
2018 ◽  
Vol 8 (57) ◽  
pp. 32893-32898 ◽  
Author(s):  
Jie Lv ◽  
Binnan He ◽  
Na Wang ◽  
Meng Li ◽  
Yulong Lin
Keyword(s):  
Gold Nanoparticle ◽  
High Sensitivity ◽  
Inhibitor Screening ◽  
Dual Channel ◽  
Sensitivity And Selectivity

A novel colorimetric and fluorescent dual-channel probe was developed for acetylcholinesterase detection and inhibitor screening with high sensitivity and selectivity.

scholarly journals Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2577 ◽  
Author(s):  
Yuanying Liang ◽  
Ting Guo ◽  
Lei Zhou ◽  
Andreas Offenhäusser ◽  
Dirk Mayer
Keyword(s):  
Detection Limit ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Label Free ◽  
Dopamine Detection ◽  
Electrochemical Transistor ◽  
Sensitivity And Selectivity

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

Nitrogen doped carbon dots: mechanism investigation and their application for label free CA125 analysis

2019 ◽  
Vol 7 (19) ◽  
pp. 3053-3058 ◽  
Author(s):  
Yuanyuan Liu ◽  
Liping Jiang ◽  
Bijun Li ◽  
Xinyue Fan ◽  
Wei Wang ◽  
...  
Keyword(s):  
Nitrogen Doping ◽  
Competitive Adsorption ◽  
High Sensitivity ◽  
Label Free ◽  
Nitrogen Doped ◽  
Microwave Assisted ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Nitrogen-doped CDs (N-CDs) were firstly prepared by using pear juice as the carbon source and ethanediamine as a nitrogen doping precursor with a microwave assisted pyrolysis technique. Based on the fluorescence recovery induced by competitive adsorption and desorption, a label-free “turn on” fluorescence assay with high sensitivity and selectivity was proposed for the analysis of CA125.

scholarly journals A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2441 ◽  
Author(s):  
Xinxing Tang ◽  
Kefeng Wu ◽  
Han Zhao ◽  
Mingjian Chen ◽  
Changbei Ma
Keyword(s):  
Adenosine Deaminase ◽  
High Selectivity ◽  
High Sensitivity ◽  
Rnase H ◽  
Fluorescence Signal ◽  
Label Free ◽  
Fluorescent Assay ◽  
Adenosine Deaminase Activity ◽  
G Quadruplex ◽  
Almost All

Adenosine deaminase (ADA), able to catalyze the irreversible deamination of adenosine into inosine, can be found in almost all tissues and plays an important role in several diseases. In this work, we developed a label-free fluorescence method for the detection of adenosine deaminase activity and inhibition. In the presence of ADA, ATP has been shown to be hydrolyzed. The ATP aptamer was shown to form a G-quadruplex/thioflavin T (ThT) complex with ThT and exhibited an obvious fluorescence signal. However, the ATP aptamer could bind with ATP and exhibited a low fluorescence signal because of the absence of ADA. This assay showed high sensitivity to ADA with a detection limit of 1 U/L based on an SNR of 3 and got a good linear relationship within the range of 1–100 U/L with R2 = 0.9909. The LOD is lower than ADA cutoff value (4 U/L) in the clinical requirement and more sensitive than most of the reported methods. This technique exhibited high selectivity for ADA against hoGG I, UDG, RNase H and λexo. Moreover, this strategy was successfully applied for assaying the inhibition of ADA using erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) and, as such, demonstrated great potential for the future use in the diagnosis of ADA-relevant diseases, particularly in advanced drug development.

Exonuclease III assisted and label-free detection of mercury ion based on toehold strand displacement amplification strategy

2016 ◽  
Vol 8 (39) ◽  
pp. 7054-7060 ◽  
Author(s):  
Lili Yu ◽  
Hui Xu ◽  
Hou Chen ◽  
Liangjiu Bai ◽  
Wenxiang Wang
Keyword(s):  
High Sensitivity ◽  
Mercury Ion ◽  
Fluorometric Assay ◽  
Label Free ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
Amplification Strategy

A label-free, exonuclease III assisted Hg2+ fluorometric assay based on strand displacement amplification was developed with high sensitivity and selectivity.

scholarly journals Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Shuchen Hsieh ◽  
Shu-Ling Hsieh ◽  
Chiung-wen Hsieh ◽  
Po-Chiao Lin ◽  
Chun-Hsin Wu
Keyword(s):  
Specific Interaction ◽  
High Sensitivity ◽  
Sensor Technology ◽  
Glucose Detection ◽  
Label Free ◽  
Con A ◽  
Cantilever Sensor ◽  
Sensitivity And Selectivity ◽  
Free Glucose ◽  
Afm Cantilever

Efficient maintenance of glucose homeostasis is a major challenge in diabetes therapy, where accurate and reliable glucose level detection is required. Though several methods are currently used, these suffer from impaired response and often unpredictable drift, making them unsuitable for long-term therapeutic practice. In this study, we demonstrate a method that uses a functionalized atomic force microscope (AFM) cantilever as the sensor for reliable glucose detection with sufficient sensitivity and selectivity for clinical use. We first modified the AFM tip with aminopropylsilatrane (APS) and then adsorbed glucose-specific lectin concanavalin A (Con A) onto the surface. The Con A/APS-modified probes were then used to detect glucose by monitoring shifts in the cantilever resonance frequency. To confirm the molecule-specific interaction, AFM topographical images were acquired of identically treated silicon substrates which indicated a specific attachment for glucose-Con A and not for galactose-Con A. These results demonstrate that by monitoring the frequency shift of the AFM cantilever, this sensing system can detect the interaction between Con A and glucose, one of the biomolecule recognition processes, and may assist in the detection and mass quantification of glucose for clinical applications with very high sensitivity.

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