An AluI PCR-RFLP detecting a silent allele at the goat POU1F1 locus and its association with production traits

2007 ◽  
Vol 73 (1-3) ◽  
pp. 8-12 ◽  
Author(s):  
X.Y. Lan ◽  
C.Y. Pan ◽  
H. Chen ◽  
C.L. Zhang ◽  
J.Y. Li ◽  
...  
Keyword(s):  
Production Traits ◽  
Pcr Rflp ◽  
Silent Allele

Association of genetic variants of the prolactin gene with milk production traits in Russian Red Pied cattle

2007 ◽  
Vol 2007 ◽  
pp. 156-156 ◽  
Author(s):  
Masoud Alipanah ◽  
Lobov Kalashnikova ◽  
Genadi Rodionov
Keyword(s):  
Genetic Marker ◽  
Regulatory Function ◽  
Cdna Clones ◽  
Production Traits ◽  
Coding Region ◽  
Nucleotide Substitutions ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Rsai Polymorphism

Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential genetic marker of production traits in dairy cattle. The gene was mapped on chromosome 23 by Hallerman et al. (1988). It consists of 5 exons and four introns (Camper et al. 1984) encoding the 199-amino-acid mature protein (Wallis 1974). On the basis of sequence analysis of four different cDNA clones, seven possible nucleotide substitutions were described by Sasavage et al. (1982). One of them, recognized by RsaI endonuclease, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (Mitra et al., 1995). Two allelic variants (B and b) have been distinguished at the DNA level, based on RsaI polymorphism in the third exon of the coding region. It has been suggested that prolactin alleles correlate with milk yield (Lewin et al., 1992).

A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene

2007 ◽  
Vol 87 (1) ◽  
pp. 13-14 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
C. Z. Lei
Keyword(s):  
Genetic Variation ◽  
Gene Polymorphism ◽  
Key Words ◽  
Milk Yield ◽  
Dairy Goat ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Silent Allele

We described a DdeI PCR-RFLP method for detecting silent allele at goat POU1F1 locus: p.S241S. Frequencies of D1 allele varied from 0.600 to 1.000 in eight Chinese native breeds. Association of DdeI RFLP genotypes with milk yield of dairy goat was significant (P < 0.05). Key words: Goat, POU1F1 gene, polymorphism, association, milk yield

scholarly journals Genetic Polymorphisms of Cyp19 and Myostatin Genes in Turkish Indigenous Sheep Breeds

2018 ◽  
Vol 68 (3) ◽  
pp. 313
Author(s):  
I. AKIŞ ◽  
F. ESEN GÜRSEL ◽  
N. HACIHASANOĞLU ÇAKMAK ◽  
G. ATMACA ◽  
H. YARDIBI ◽  
...  
Keyword(s):  
Growth Traits ◽  
Muscle Growth ◽  
Reproductive Activity ◽  
Meat Production ◽  
Production Traits ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Estrogen Synthesis ◽  
Pcr Rflp ◽  
Cyp19 Gene

Growth and meat production traits are very important in sheep breeding. Cyp19 gene has a major role in reproductive activity and growth due to its function in estrogen synthesis. Another gene affecting growth traits is  Myostatin (MSTN) gene, which mainly regulates skeletal muscle growth. In this study allele frequencies of genetic polymorphism in Cyp19 and Myostatin genes were identified by PCR-RFLP method in five indigenous Turkish sheep breeds, Chiose, Imroz, Kivircik, Zom and Morkaraman. Digestion of Cyp19 gene with HaeIII only revealed uncut AA genotype and digestion of MSTN with DraI also revealed only uncut AA genotype. Both loci analyzed in this study were found to be monomorphic in five Turkish indigenous sheep breeds. These highly conserved parts of the two genes can be useful for molecular evolutionary studies in sheep. Further studies regarding association analysis of Cyp19 and MSTN in sheep should be conducted.

scholarly journals PCR-SSCP and <i>Hin6</i>I PCR-RFLP detecting novel anonymous SNPs within ovine <i>PROP1</i> gene (Brief report)

2008 ◽  
Vol 51 (6) ◽  
pp. 622-623
Author(s):  
X. Lan ◽  
C. Pan ◽  
S. Hu ◽  
H. Chen
Keyword(s):  
Thyroid Stimulating Hormone ◽  
Animal Breeding ◽  
Potential Candidate ◽  
Production Traits ◽  
Coding Regions ◽  
Potential Candidate Gene ◽  
Pcr Rflp ◽  
Ruminant Animals ◽  
Prop1 Gene ◽  
Breeding And Genetics

Abstract. PROP1 plays a direct or indirect key role in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes (WU et al., 1998). Mutations of PROP1 are responsible for deficiencies of POU1F1, GH, prolactin (PRL) and thyroid stimulating hormone (TSH) in Snell and Jackson dwarf mice and in man, as well as deficiencies of LH and FSH, suggesting that the PROP1 gene is a potential candidate gene associating with production traits in animal breeding and genetics. To date, no polymorphism of PROP1 gene has been reported in ruminant animals except bovine (PAN et al., 2007). So, the objective of this study is to investigate the polymorphism of all coding regions within ovine PROP1 gene by PCR-SSCP, DNA sequencing and PCR-RFLP methods, which will possibly contribute to conducting association analysis and evaluating them as genetic markers in animal breeding and genetics.

scholarly journals GENETIC ANALYSIS OF UKRAINIAN LARGE WHITE PIG BREED BY POLYMORPHISMS IN RYR1, ESR1 AND PRLR GENES

2021 ◽  
pp. 30-36
Author(s):  
Y. K. Oliinychenko ◽  
V.I. Rossokha ◽  
O. A. Boyko ◽  
G. N. Tur ◽  
O. A. Zaderikhina ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Economic Traits ◽  
Large White ◽  
Polymorphic Snps ◽  
Pcr Rflp ◽  
Productive Traits ◽  
Pig Performance ◽  
Large White Pig

Marker-Assisted Selection is getting increasing attention in Ukrainian animal breeding as an effective tool for choosing animals with desirable traits. Identification of molecular markers which are related to major genes is a promising approach for improving economic traits and has to be checked for further gene-trait associations. Single nucleotide polymorphisms are genetic markers that can be associated with production traits. SNP genotyping has to be done additionally for each breed to see if they are polymorphic and have significant associations with certain traits. Among the candidate genes that influence the expression of productive traits, special attention is drawn to RYR1, ESR1 and PRLR genes. Gene RYR1 is associated with muscle hypertrophy and other meat quality defects in pigs. ESR1 and PRLR genes influence reproduction and affect the litter size in pigs. SNPs RYR1 g.1843 C>T, ESR1 SNP c.1227 C>T and PRLR g. 1789 G>A have been receiving increasing attention as potential markers for improving quality and pig performance traits. This study investigated the allele variants in three genes (RYR1, ESR1 and PRLR) in Ukrainian Large White pigs of different inside breed types (ULW-1 and ULW-2). The study was performed on 200 pigs. Genotyping was performed using PCR–RFLP method. SNP RYR1 g.1843 C>T was not polymorphic in ULW-1 and ULW-2 inside breed types, when ESR1 SNP c.1227 C> T and PRLR g. 1789 G>A were highly polymorphic. SNPs in ESR1 and PRLR genes had high PIC and χ2 values which indicates the relevance of further associative studies in ULW-1 and ULW-2. Informativeness of the genetic markers in ESR1 and PRLR genes was optimal for associative studies in Ukrainian Large White pig breed of first and second inside breed type.

scholarly journals Genetic diversity of <i>κ</i>-casein (<i>CSN3</i>) and lactoferrin (<i>LTF</i>) genes in the endangered Turkish donkey (<i>Equus asinus</i>) populations

2019 ◽  
Vol 62 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Fulya Özdil ◽  
Hasan Bulut ◽  
Raziye Işık
Keyword(s):  
Restriction Enzyme ◽  
Animal Species ◽  
Restriction Enzymes ◽  
Production Traits ◽  
Single Nucleotide ◽  
Equus Asinus ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Polymerase Chain ◽  
Enzyme Recognition

Abstract. In this study, the κ-casein (CSN3) and lactoferrin (LTF) genes which were found in association with milk production traits in different animal species were studied firstly in Turkish donkey populations. A total of 108 donkeys from different regions of Turkey were used in order to reveal the different genotypes of CSN3 and LTF genes by using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods. To determine the genetic polymorphism, we attempted to digest a fragment of 235 bp of the CSN3 gene and a fragment of 751 bp of the LTF gene using PstI, and DraII, EagI and MboI restriction enzymes, respectively. Neither the CSN3 gene nor the LTF gene had enzyme recognition sites with the PstI, DraII and MboI restriction enzymes in all of the studied samples. However, the LTF gene was only distinguished with the EagI restriction enzyme. Three genotypes were identified in the LTF gene with the EagI restriction enzyme: GG homozygotes (667, 84 bp), AG heterozygotes (751; 667, 84 bp) and AA homozygotes (751 bp). The transition from guanine to adenine in 89 bp of the LTF gene lacks the restriction site and different genotypes are obtained. This novel single nucleotide polymorphism (SNP) has been firstly detected in donkeys. According to the results, the G allele was predominant in the LTF-EagI gene in the studied Turkish donkey populations. In this study, all the genotype distributions of LTF-EagI were not found in Hardy–Weinberg equilibrium (P<0.05). The CSN3 and LTF genes have not been studied before in donkeys, so the results are the preliminary results of these gene regions in donkeys.

scholarly journals Associations between the bovine PPARGC1A gene and milk production traits

2010 ◽  
Vol 55 (No. 5) ◽  
pp. 195-199 ◽  
Author(s):  
I. Kowalewska-Łuczak ◽  
H. Kulig ◽  
M. Kmieć
Keyword(s):  
Protein Content ◽  
Milk Production ◽  
Common Haplotype ◽  
Production Traits ◽  
Milk Production Traits ◽  
Milk Traits ◽  
Pcr Rflp ◽  
The Individual ◽  
Jersey Cows

The aim of this study was to investigate associations between PPARGC1A genotypes and haplotypes and milk production traits. The study included 181 Jersey cows. The genotypes were identified by the PCR-RFLP method. The frequencies of the most common alleles were as follows: T &ndash; 0.63 (c.1892T&gt;C) and A &ndash; 0.88 (c.3359A&gt;C). The frequency of the most common haplotype was TC/AA &ndash; 0.558. The results showed that there were no statistically significant associations between the individual genotypes of both SNPs and milk traits; however, individuals with the CC/AC haplotype produced significantly (P &le; 0.05; P &le; 0.001) less milk and had a significantly (P &le; 0.001) higher protein content in milk.

scholarly journals Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 123 ◽  
Author(s):  
Katarzyna Piórkowska ◽  
Martyna Małopolska ◽  
Katarzyna Ropka-Molik ◽  
Magdalena Szyndler-Nędza ◽  
Angelika Wiechniak ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Missense Variant ◽  
Intramuscular Fat ◽  
Regulatory Function ◽  
Pork Quality ◽  
Production Traits ◽  
Rna Seq ◽  
Feed Conversion ◽  
Meat Content ◽  
Pcr Rflp

In recent years, pig producers have struggled with the problem of low intramuscular fat levels in pork, which impacts palatability and ultimately meat quality. Reduced levels of intramuscular fat are likely the result of breeding objectives aimed at increasing lean meat content. In this study, three mutations within candidate genes for fat content (SCD, ACACA, and FASN) were selected, based on RNA-seq results and the relationship between polymorphisms in genes related to lipid metabolism, fattening and slaughter characteristics, as well as pork quality, including IMF level, were evaluated to identify selection markers. Moreover, their impact on gene expression was also examined. The PCR–RFLP (polymerase cha- in reaction – restriction fragments length) method was used to establish genotypes and effect sizes of potential genetic markers were estimated using a GLM model. It was identified that a FASN missense variant was positively associated with the expression level of this gene, which suggested its linkage with a mutation having a regulatory function. The association study indicated that the FASN missense variant may play a role in the determination of feed conversion and meat colour. In turn, a mutation in the ACACA gene showed a relationship with IMF content in the Puławska breed where the differences reached as much as 20%. We suggest considering all three mutations in further studies based on different pig populations due to the crucial role of SCD, ACACA, and FASN genes in lipid metabolism.

Association of polymorphism at intron 2 of FABP3 Gene with milk production traits in Sahiwal and Karan Fries cattle

2018 ◽  
Author(s):  
Alok Kumar Yadav ◽  
Anupama Mukherjee ◽  
Suchit Kumar
Keyword(s):  
Milk Production ◽  
Rflp Analysis ◽  
Production Traits ◽  
Milk Production Traits ◽  
Pcr Rflp ◽  
Sahiwal Cattle ◽  
Pcr Products ◽  
The Mean ◽  
Intron 2 ◽  
Karan Fries

PCR-RFLP analysis of PCR products were carried out using Aci I / SSi I for 100 Sahiwal and 115 Karan Fries cattle. In Sahiwal cattle and Karan Fries cattle, 438bp has three genotypes AA (438), AB (438+299+139 bp) and BB (299+139 bp). In Sahiwal cattle these genotypes are highly significant for FL305DPY but in Karan Fries cattle these genotypes are highly significant for FL305DMY, FLTMY, FL305DFY and FL305DPY. In Sahiwal cattle, mean ± SE of AA genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY, FL305DPY were 1809.90 ± 15.7 kg, 2029.4 ± 15.6 kg, 99.90 ± 0.66 kg, 154.87 ± 0.17 kg and 44.81 ± 0.06 kg, respectively and for AB genotype were 1800.76 ± 9.48 kg, 1993.99 ± 9.42 kg, 100.54 ± 0.39 kg, 154.79 ± 0.10 kg, 43.99 ± 0.04 kg, respectively and for BB genotype were 1830.0 ± 14.10 kg, 2032.80 ± 14.0 kg, 100.24 ± 0.59 kg, 155.11 ± 0.15 kg, 42.98 ± 0.05 kg, respectively. Heterozygous AB genotype was found to be superior for, FL305DFY trait. AA genotype was significantly superior for FL305DPY traits whereas BB genotype was found to be superior for FL305DMY, FLTMY, FL305DSNFY. In Karan Fries cattle, the mean ± SE of AA genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY, FL305DPY were 3442.17 ± 8.39 kg, 4461.93 ± 8.39 kg, 124.96 ± 7.20 kg, 277.35 ± 0.08 kg and 112.51 ± 0.08 kg, respectively and for AB genotype were 3572.69 ± 5.93 kg, 4592.45 ± 5.93 kg, 140.17 ± 5.09 kg, 278.60 ± 0.06 kg, 113.91 ± 0.05 kg, respectively and for BB genotypes were 3502.41 ± 9.19 kg, 4522.17 ± 9.19 kg, 136.91 ± 7.89 kg, 277.93 ± 0.09 kg, 113.19 ± 0.08 kg, respectively.

Identification of Genetic Variants in ABCG2 Gene Influencing Milk Production Traits in Dairy Cattle

2019 ◽  
Author(s):  
Arun Pratap Singh ◽  
A K Chakravarty ◽  
M A Mir ◽  
Ashwani Arya ◽  
Manvendra Singh
Keyword(s):  
Milk Yield ◽  
Rflp Analysis ◽  
Milk Composition ◽  
Breeding Value ◽  
Production Traits ◽  
Crossbred Cattle ◽  
Fat Percentage ◽  
Pcr Rflp ◽  
Karan Fries ◽  
Abcg2 Gene

This study was performed to investigate the polymorphisms in the ABCG2 (ATP-binding cassette sub-family G member 2) gene and to reveal the association of genotypes with breeding value (BV) for first lactation milk yield and milk composition traits of Karan Fries (HF crossbred) cattle. The traits were adjusted against the significant effect of non-genetic factors. PCR-RFLP analysis of ABCG2 (exon 14) gene revealed three genotypes. AA genotype in ABCG2 gene had significant effect on BV for average test day fat percentage. The identified potential genetic marker could be used for the development of Marker Assisted Selection (MAS) strategy for higher milk yield and milk composition traits in Karan Fries Cattle.

Sign in / Sign up

Export Citation Format

Share Document