Pumpkin seed protein hydrolysate treatment alleviates salt stress effects on Phaseolus vulgaris by elevating antioxidant capacity and recovering ion homeostasis

2020 ◽  
Vol 271 ◽  
pp. 109495 ◽  
Author(s):  
Mahmoud Z. Sitohy ◽  
El-Sayed M. Desoky ◽  
Ali Osman ◽  
Mostafa M. Rady
Keyword(s):  
Salt Stress ◽  
Phaseolus Vulgaris ◽  
Antioxidant Capacity ◽  
Seed Protein ◽  
Protein Hydrolysate ◽  
Ion Homeostasis ◽  
Pumpkin Seed ◽  
Stress Effects

Exogenous application of 24-epibrassinosteroid mitigates NaCl toxicity in flax by modifying free amino acids profile and antioxidant defence system

2020 ◽  
Vol 47 (6) ◽  
pp. 565 ◽  
Author(s):  
Leila Amraee ◽  
Fatemeh Rahmani ◽  
Babak Abdollahi Mandoulakani
Keyword(s):  
Amino Acids ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Antioxidant Capacity ◽  
Free Amino ◽  
Ion Homeostasis ◽  
Seed Priming ◽  
Antioxidant Response ◽  
Salinity Treatment ◽  
Antioxidant Defence System

In the present study, we investigated the ameliorative effects of 24-epibrassinosteroid (24-epiBL) on antioxidant response and ion homeostasis in two NaCl-stressed Linum usitatissimum L. (flax) cultivars differing in salt tolerance. The content and profile of amino acids were also studied in the tolerant cultivar. Salt stress differently altered the activity of antioxidant enzymes, phenol and flavonoid contents, total antioxidant capacity and ion homeostasis in both cultivars, whereas H2O2 and malondialdehyde (MDA) contents were induced only in the TN-97-95 cultivar. Free amino acid concentrations showed variable patterns under salinity conditions compared with the control plants. 24-epiBL decreased the soluble protein content in NaCl-treated plants and also decreased stimulatory effects of salinity on the production and accumulation of phenol and flavonoid contents and antioxidant capacity with altered ion (Na+, K+, and Cl–) contents. The 24-epiBL reduced the chlorophylls (a, b) and carotenoid contents in salt-treated TN-97-95 cultivar while enhanced the activity of antioxidant enzymes and declined the H2O2 content and lipid peroxidation in both NaCl-stressed cultivars. The profile and content of amino acids were significantly changed by 24-epiBL application under salinity treatment. In summary, our findings demonstrate that 24-epiBL seed priming mitigates the deleterious effects of salt stress in flax plants.

scholarly journals Functional properties of pumpkin (Cucurbita pepo) seed protein isolate and hydrolysate

2016 ◽  
Vol 81 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Sandra Bucko ◽  
Jaroslav Katona ◽  
Ljiljana Popovic ◽  
Zuzana Vastag ◽  
Lidija Petrovic
Keyword(s):  
Ionic Strength ◽  
Cucurbita Pepo ◽  
Seed Protein ◽  
Protein Hydrolysate ◽  
Protein Isolate ◽  
Emulsifying Properties ◽  
Protein Solution ◽  
Pumpkin Seed ◽  
Solution Interface ◽  
Oil In Water

Pumpkin seed protein isolate (PSPI) was enzymatically hydrolysed by pepsin to obtain pumpkin seed protein hydrolysate, PSPH. Investigation on solubility, interfacial and emulsifying properties of both PSPI and PSPH was conducted under different conditions of pH (3-8) and ionic strength (0-1 mol/dm3 NaCl). PSPI had the lowest solubility, i.e. isoelectric point (pI), at pH 5. PSPH had higher solubility than PSPI over whole range of pH and ionic strengths tested. Decrease in surface and interfacial tension evidenced that both PSPI and PSPH adsorb at air/protein solution and oil/protein solution interface. Emulsions (20 % oil in water) stabilized by 1 g/100cm3 PSPI or PSPH solution were prepared at pH 3, 5 and 8 and ionic strength of 0 and 0.5 mol/dm3 NaCl. PSPH stabilized emulsions from coalescence at all pH and ionic strengths tested. PSPI was able to stabilize emulsions at pH 3 and 0 mol/dm3 NaCl, and at pH 8 regardless of ionic strength, while emulsions at pH 5 and both 0 and 0.5 mol/dm3 NaCl and at pH 3 when ionic strength was increased separated to oil and serum layer immediately after preparation. All emulsions were susceptible to creaming instability.

scholarly journals An investigation on the influence of pH and ionic strength on the adsorption and interfacial dilatational properties at the oil-water interface of pumpkin (Cucurbita pepo) seed protein hydrolysate

2018 ◽  
Vol 83 (7-8) ◽  
pp. 847-861
Author(s):  
Sandra Bucko ◽  
Jaroslav Katona ◽  
Lidija Petrovic ◽  
Jelena Milinkovic ◽  
Jadranka Fraj ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Rate Constant ◽  
Cucurbita Pepo ◽  
Seed Protein ◽  
Protein Hydrolysate ◽  
Protein Isolate ◽  
Pumpkin Seed ◽  
Interfacial Pressure ◽  
Oscillation Frequencies ◽  
Influence Of Ph

Pumpkin (Cucurbita pepo) seed protein hydrolysate (PSPH) was obtained by enzymatic hydrolysis of pumpkin seed protein isolate using pepsin. Influence of pH (3, 5 and 8) and ionic strength, Ic (0?1 mol dm-3), on the adsorption kinetics of PSPH (diffusion rate constant, kdiff, and adsorption rate constant, kads), interfacial pressure (?) and interfacial dilatational properties (dilatational elasticity, E?, and viscosity, E?) of the oil?PSPH solution interfaces was investigated at different PSPH concentrations (c = 0.0014?14 g dm-3). It was found that PSPH adsorbs to the interface at c ? 0.0014 g dm-3, regardless of pH and ionic strength, as evidenced by the increase in interfacial pressure. The kdiff and kads value were found to be the highest at pH 3 and the lowest at pH 5 at the corresponding concentrations. The dilatational properties of the interfaces, which were investigated at different oscillation frequencies, ?, 0.01?0.2 Hz, showed that the E? of the oil?PSPH solution interfaces is much higher than its E?. Moreover, E? increases with increasing PSPH concentration at pH 5 and 8, and with increasing Ic, regardless of the pH, while E? changes only minimally.

scholarly journals Ethylene Supplementation Combined with Split Application of Nitrogen and Sulfur Protects Salt-Inhibited Photosynthesis through Optimization of Proline Metabolism and Antioxidant System in Mustard (Brassica juncea L.)

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1303
Author(s):  
Badar Jahan ◽  
Noushina Iqbal ◽  
Mehar Fatma ◽  
Zebus Sehar ◽  
Asim Masood ◽  
...  
Keyword(s):  
Salt Stress ◽  
Brassica Juncea ◽  
Antioxidant System ◽  
Photosynthetic Efficiency ◽  
Growth Potential ◽  
Proline Metabolism ◽  
Sowing Time ◽  
Stress Effects ◽  
Nutrient Assimilation ◽  
S Application

In the present study, the potential of ethylene as ethephon (an ethylene source) was investigated individually and in combination with split doses of nitrogen (N) and sulfur (S) soil treatments for removal of the damaging effects of salt stress (100 mM NaCl) in mustard (Brassica juncea L.). Plants were grown with 50 mg N plus 50 mg S kg−1 soil at sowing time and an equivalent dose at 20 days after sowing [N50 + S50]0d and 20d. Ethephon at 200 μL L‒1 was applied to combined split doses of N and S with or without NaCl. Plants subjected to NaCl showed a decrease in growth and photosynthetic characteristics as well as N and S assimilation, whereas proline metabolism and antioxidants increased. The application of ethephon to plants grown with split N and S doses significantly enhanced photosynthetic efficiency by increasing the assimilation of N and S, improving the concentration of proline and induction of the antioxidant system with or without NaCl. The regulation of ethylene and/or split forms of N and S application may be potential tools for not just overcoming salt stress effects in this species and in related Brassicaceae but also enhancing their photosynthesis and growth potential through increased nutrient assimilation.

Ion homeostasis during salt stress in plants

2001 ◽  
Vol 13 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Ramón Serrano ◽  
Alonso Rodriguez-Navarro
Keyword(s):  
Salt Stress ◽  
Ion Homeostasis
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