Exogenous caffeic acid and epicatechin enhance resistance against Botrytis cinerea through activation of the phenylpropanoid pathway in apples

2020 ◽  
Vol 268 ◽  
pp. 109348
Author(s):  
Mengyu Zhang ◽  
Dajiang Wang ◽  
Xixi Gao ◽  
Zhengyang Yue ◽  
Huiling Zhou
Keyword(s):  
Botrytis Cinerea ◽  
Caffeic Acid ◽  
Phenylpropanoid Pathway

Effect of nitrous oxide against Botrytis cinerea and phenylpropanoid pathway metabolism in table grapes

2019 ◽  
Vol 254 ◽  
pp. 99-105 ◽  
Author(s):  
Jing Xu ◽  
Zheng Zhang ◽  
Xueping Li ◽  
Jia Wei ◽  
Bin Wu
Keyword(s):  
Nitrous Oxide ◽  
Botrytis Cinerea ◽  
Phenylpropanoid Pathway ◽  
Table Grapes

scholarly journals Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis

2006 ◽  
Vol 188 (7) ◽  
pp. 2666-2673 ◽  
Author(s):  
Martin Berner ◽  
Daniel Krug ◽  
Corina Bihlmaier ◽  
Andreas Vente ◽  
Rolf Müller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heterologous Expression ◽  
Caffeic Acid ◽  
Biosynthetic Pathway ◽  
Phenylpropanoid Pathway ◽  
Hydroxycinnamic Acid ◽  
Coumaric Acid ◽  
Streptomyces Fradiae ◽  
Acid Biosynthesis ◽  
Tyrosine Ammonia Lyase

ABSTRACT The saccharomicins A and B, produced by the actinomycete Saccharothrix espanaensis, are oligosaccharide antibiotics. They consist of 17 monosaccharide units and the unique aglycon N-(m,p-dihydroxycinnamoyl)taurine. To investigate candidate genes responsible for the formation of trans-m,p-dihydroxycinnamic acid (caffeic acid) as part of the saccharomicin aglycon, gene expression experiments were carried out in Streptomyces fradiae XKS. It is shown that the biosynthetic pathway for trans-caffeic acid proceeds from l-tyrosine via trans-p-coumaric acid directly to trans-caffeic acid, since heterologous expression of sam8, encoding a tyrosine ammonia-lyase, led to the production of trans-p-hydroxycinnamic acid (coumaric acid), and coexpression of sam8 and sam5, the latter encoding a 4-coumarate 3-hydroxylase, led to the production of trans-m,p-dihydroxycinnamic acid. This is not in accordance with the general phenylpropanoid pathway in plants, where trans-p-coumaric acid is first activated before the 3-hydroxylation of its ring takes place.

scholarly journals Activite Antifongique Des Flavonoïdes Isoles De La Plante Asteriscus Graveolens Subsp. Odorus (Schousb.) Greuter

2016 ◽  
Vol 12 (12) ◽  
pp. 258
Author(s):  
Hakim Alilou ◽  
Bouchaib Bencharki ◽  
Jalal Talbi ◽  
Noureddine Barka
Keyword(s):  
Botrytis Cinerea ◽  
Caffeic Acid ◽  
Fruits And Vegetables ◽  
High Sensitivity ◽  
Penicillium Digitatum ◽  
Penicillium Expansum ◽  
Activité Antifongique ◽  
Post Harvest ◽  
The Subject

A phenol acid and three flavones isolated from the plant Asteriscus graveolens subsp. odorus were tested against three fungi: Botrytis cinerea, Penicillium digitatum and Penicillium expansum. Using the method of incorporation of products in a PDA, a high sensitivity of Botrytis cinerea to caffeic acid and nevadensine was observed. Penicillium digitatum is the sensitive to luteolin, as to Penicillium expansum, it marks a sensitivity to artemetin. The efficacy of the tested molecules could be the subject of an investigation and exploitation in the integrated fight against the three tested fungi that cause a lot of damage to fruits and vegetables post-harvest.

scholarly journals 4-Coumaroyl-CoA ligases in the biosynthesis of the anti-diabetic metabolite montbretin A

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0257478
Author(s):  
Frederick G. Sunstrum ◽  
Hannah L. Liu ◽  
Sharon Jancsik ◽  
Lufiani L. Madilao ◽  
Joerg Bohlmann ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Caffeic Acid ◽  
Expression Patterns ◽  
Differential Expression Analysis ◽  
Lignin Biosynthesis ◽  
Building Blocks ◽  
Phenylpropanoid Pathway ◽  
Major Product ◽  
Hydroxyl Group

Background Montbretins are rare specialized metabolites found in montbretia (Crocosmia x crocosmiiflora) corms. Montbretin A (MbA) is of particular interest as a novel therapeutic for type-2 diabetes and obesity. There is no scalable production system for this complex acylated flavonol glycoside. MbA biosynthesis has been reconstructed in Nicotiana benthamiana using montbretia genes for the assembly of MbA from its various different building blocks. However, in addition to smaller amounts of MbA, the therapeutically inactive montbretin B (MbB) was the major product of this metabolic engineering effort. MbA and MbB differ in a single hydroxyl group of their acyl side chains, which are derived from caffeoyl-CoA and coumaroyl-CoA, respectively. Biosynthesis of both MbA and MbB also require coumaroyl-CoA for the formation of the myricetin core. Caffeoyl-CoA and coumaroyl-CoA are formed in the central phenylpropanoid pathway by acyl activating enzymes (AAEs) known as 4-coumaroyl-CoA ligases (4CLs). Here we investigated a small family of montbretia AAEs and 4CLs, and their possible contribution to montbretin biosynthesis. Results Transcriptome analysis for gene expression patterns related to montbretin biosynthesis identified eight different montbretia AAEs belonging to four different clades. Enzyme characterization identified 4CL activity for two clade IV members, Cc4CL1 and Cc4CL2, converting different hydroxycinnamic acids into the corresponding CoA thioesters. Both enzymes preferred coumaric acid over caffeic acid as a substrate in vitro. While expression of montbretia AAEs did not enhance MbA biosynthesis in N. benthamiana, we demonstrated that both Cc4CLs can be used to activate coumaric and caffeic acid towards flavanone biosynthesis in yeast (Saccharomyces cerevisiae). Conclusions Montbretia expresses two functional 4CLs, but neither of them is specific for the formation of caffeoyl-CoA. Based on differential expression analysis and phylogeny Cc4CL1 is most likely involved in MbA biosynthesis, while Cc4CL2 may contribute to lignin biosynthesis. Both Cc4CLs can be used for flavanone production to support metabolic engineering of MbA in yeast.

The phenylpropanoid pathway affects apple fruit resistance to Botrytis cinerea

2017 ◽  
Vol 166 (3) ◽  
pp. 206-215 ◽  
Author(s):  
Lijing Ma ◽  
Junhua He ◽  
Huan Liu ◽  
Huiling Zhou
Keyword(s):  
Botrytis Cinerea ◽  
Phenylpropanoid Pathway ◽  
Apple Fruit

scholarly journals Transcriptome-Wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of Its Putative BAHDs Gene Complex in A. spathulifolius

2021 ◽  
Vol 22 (12) ◽  
pp. 6333
Author(s):  
Sivagami-Jean Claude ◽  
Sunmi Park ◽  
Seon-Joo Park
Keyword(s):  
Phylogenetic Analysis ◽  
Abiotic Stress ◽  
Human Health ◽  
Caffeic Acid ◽  
Phenylpropanoid Pathway ◽  
Biosynthesis Pathway ◽  
Gene Complex ◽  
Biotic And Abiotic Stress ◽  
Acid Biosynthesis ◽  
Caffeoylquinic Acid

The phenylpropanoid pathway is a major secondary metabolite pathway that helps plants overcome biotic and abiotic stress and produces various byproducts that promote human health. Its byproduct caffeoylquinic acid is a soluble phenolic compound present in many angiosperms. Hydroxycinnamate-CoA shikimate/quinate transferase is a significant enzyme that plays a role in accumulating CQA biosynthesis. This study analyzed transcriptome-wide identification of the phenylpropanoid to caffeoylquinic acid biosynthesis candidate genes in A. spathulifolius flowers and leaves. Transcriptomic analyses of the flowers and leaves showed a differential expression of the PPP and CQA biosynthesis regulated unigenes. An analysis of PPP-captive unigenes revealed a major duplication in the following genes: PAL, 120 unigenes in leaves and 76 in flowers; C3′H, 169 unigenes in leaves and 140 in flowers; 4CL, 41 unigenes in leaves and 27 in flowers; and C4H, 12 unigenes in leaves and 4 in flowers. The phylogenetic analysis revealed 82 BAHDs superfamily members in leaves and 72 in flowers, among which five unigenes encode for HQT and three for HCT. The three HQT are common to both leaves and flowers, whereas the two HQT were specialized for leaves. The pattern of HQT synthesis was upregulated in flowers, whereas HCT was expressed strongly in the leaves of A. spathulifolius. Overall, 4CL, C4H, and HQT are expressed strongly in flowers and CAA and HCT show more expression in leaves. As a result, the quantification of HQT and HCT indicates that CQA biosynthesis is more abundant in the flowers and synthesis of caffeic acid in the leaves of A. spathulifolius.

An Attempt at Biological Control of Blossom Blight of Rose Caused by Botrytis cinerea Using some Local Trichoderma spp. Strains

2020 ◽  
Vol 55 (1) ◽  
pp. 27-34
Author(s):  
G. Zadehdabagh ◽  
K. Karimi ◽  
M. Rezabaigi ◽  
F. Ajamgard
Keyword(s):  
Botrytis Cinerea ◽  
Mycelial Growth ◽  
Limiting Factor ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Khuzestan Province ◽  
Inhibitory Potential ◽  
Cut Rose

The northern of Khuzestan province in Iran is mainly considered as one of the major areas of miniature rose production. Blossom blight caused by Botrytis cinerea has recently become a serious limiting factor in rose production in pre and post-harvest. In current study, an attempt was made to evaluate the inhibitory potential of some local Trichoderma spp. strains against B. cinerea under in vitro and in vivo conditions. The in vitro results showed that all Trichoderma spp. strains were significantly able to reduce the mycelial growth of the pathogen in dual culture, volatile and non-volatile compounds tests compared with control, with superiority of T. atroviride Tsafi than others. Under in vivo condition, the selected strain of T. atroviride Tsafi had much better performance than T. harzianum IRAN 523C in reduction of disease severity compared with the untreated control. Overall, the findings of this study showed that the application of Trichoderma-based biocontrol agents such as T. atroviride Tsafi can be effective to protect cut rose flowers against blossom blight.

Persistence of modern varieties and hybrids of carrots and its dependence on the biochemical composition

2020 ◽  
pp. 16-19
Author(s):  
Е.В. Янченко
Keyword(s):  
Botrytis Cinerea ◽  
Sclerotinia Sclerotiorum ◽  
Dry Matter ◽  
Inverse Correlation ◽  
Dry Weight ◽  
Matter Content ◽  
White Rot ◽  
Dry Matter Content ◽  
Important Indicator ◽  
Modern Varieties

Цель исследований – дать оценку сохраняемости и болезнеустойчивости современных сортов и гибридов моркови столовой и определить корреляционные зависимости влияния биохимических показателей качества на сохраняемость и степень поражения моркови столовой различными видами болезней в процессе хранения. Исследования проводились в 2011–2016 годах во ВНИИО – филиале ФГБНУ ФНЦО по общепринятым методикам. В биохимической лаборатории отдела земледелия и агрохимии содержание сухого вещества определяли высушиванием до абсолютно сухого веса, общего сахара – по Бертрану, аскорбиновой кислоты – по Мурри, нитраты – ионоселективным методом. При характеристике моркови столовой важнейший показатель, определяющий его качество – количество сухого вещества и сахаров. В процессе хранения были выявлены следующие болезни моркови: серая гниль (Botrytis cinerea Pers. ex Fr.), белая гниль (Sclerotinia sclerotiorum (Lib.)), белая парша (Rhizoctonia carotae Rad.), альтернариоз (Alternaria radicina M., Dr. et E.). В большей степени сортообразцы моркови столовой поражались серой гнилью. Лучшими по сохраняемости сортообразцами были Корсар (94,6%), F1Берлин (94,5%), Берликум Роял (94,1%) и F1 Звезда (94%). Сохраняемость у зарубежных сортов и гибридов моркови столовой была немного выше, чем у отечественных (на 0,4%) как за счет меньшей величины убыли массы (6,3% против 6,4%), так и потерь от болезней (1,6% против 1,9%). Сохраняемость корнеплодов моркови находится в прямой корреляционной зависимости от содержания сухого вещества (r=+0,41), каротиноидов (r=+0,39), моносахаров (r­=+0,30) и суммы сахаров (r=+0,27). Проявление серой гнили находится в обратной корреляционной связи с содержанием сухого вещества и каротиноидов (r=-0,37 и r=-0,35 соответственно), белой парши – в прямой корреляции с содержанием сухого вещества , моносахаров и дисахаров (r= +0,21; r= +0,39; r= -0,41 соответственно), белой гнили в обратной корреляционной связи с содержанием сухого вещества, моносахаров и дисахаров. The purpose of the research is to assess the persistence and disease resistance of modern varieties and hybrids of carrots and to determine the correlation between the influence of biochemical quality indicators on the persistence and degree of damage to carrots by various types of diseases during storage. The research was conducted in 2011–2016 at ARRIVG – branch of FSBSI FSVC, according to generally accepted methods. In the biochemical laboratory of the Department of Agriculture and Agrochemistry, the dry matter content was determined by drying to absolutely dry weight, total sugar – by Bertran, ascorbic acid – by Murri, nitrates – by the ion-selective method. When describing carrots, the most important indicator that determines its quality is the amount of dry matter and sugars. During storage, the following diseases of carrots were detected: gray rot (Botrytis cinerea Pers. ex Fr.), white rot (Sclerotinia sclerotiorum (Lib.), white scab (Rhizoctonia carotae Rad.), alternariasis (Alternaria radicina M., Dr. et E.). To a greater extent, varieties of table carrots were affected by gray rot. The best preserved varieties were Corsar (94.6%), F1 Berlin (94.5%), Berlicum Royal (94.1%) and F1 Zvezda (94%). The persistence of foreign varieties and hybrids of table carrots was slightly higher than that of domestic ones by 0.4%. both due to a smaller amount of weight loss (6.3% vs. 6.4%) and losses from diseases (1.6% vs. 1.9%). The persistence of carrot root crops is directly correlated with the content of dry matter (r=+0.41), carotenoids (r=+0.39), monosaccharides (r=+0.30) and the amount of sugars (r=+0.27). The manifestation of gray rot is in inverse correlation with the content of dry matter and carotenoids (r=-0.37 and r=-0.35, respectively), white scab is in direct correlation with the content of dry matter (r= +0.21; r= +0.39; r= –0.41, respectively), white rot is in inverse correlation with the content of dry matter, monosaccharides and disaccharides.

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