New insight into ovary abortion during ovary development of hazelnut through a combined proteomic and transcriptomic analysis

2018 ◽  
Vol 234 ◽  
pp. 36-48 ◽  
Author(s):  
Jianfeng Liu ◽  
Jiyang Xing ◽  
Jun Fang ◽  
Pengfei Ai ◽  
Yunqing Cheng
Keyword(s):  
Transcriptomic Analysis ◽  
Ovary Development ◽  
Insight Into

scholarly journals Proteo-Transcriptomic Analysis Identifies Potential Novel Toxins Secreted by the Predatory, Prey-Piercing Ribbon Worm Amphiporus lactifloreus

Marine Drugs ◽  
2020 ◽  
Vol 18 (8) ◽  
pp. 407 ◽  
Author(s):  
Björn Marcus von Reumont ◽  
Tim Lüddecke ◽  
Thomas Timm ◽  
Günter Lochnit ◽  
Andreas Vilcinskas ◽  
...  
Keyword(s):  
Small Molecule ◽  
Sea Anemone ◽  
Transcriptomic Analysis ◽  
Sea Star ◽  
Cystine Knot ◽  
Preliminary Identification ◽  
Protein Toxins ◽  
Insight Into

Nemerteans (ribbon worms) employ toxins to subdue their prey, but research thus far has focused on the small-molecule components of mucus secretions and few protein toxins have been characterized. We carried out a preliminary proteotranscriptomic analysis of putative toxins produced by the hoplonemertean Amphiporus lactifloreus (Hoplonemertea, Amphiporidae). No variants were found of known nemertean-specific toxin proteins (neurotoxins, cytotoxins, parbolysins or nemertides) but several toxin-like transcripts were discovered, expressed strongly in the proboscis, including putative metalloproteinases and sequences resembling sea anemone actitoxins, crown-of-thorn sea star plancitoxins, and multiple classes of inhibitor cystine knot/knottin family proteins. Some of these products were also directly identified in the mucus proteome, supporting their preliminary identification as secreted toxin components. Two new nemertean-typical toxin candidates could be described and were named U-nemertotoxin-1 and U-nemertotoxin-2. Our findings provide insight into the largely overlooked venom system of nemerteans and support a hypothesis in which the nemertean proboscis evolved in several steps from a flesh-melting organ in scavenging nemerteans to a flesh-melting and toxin-secreting venom apparatus in hunting hoplonemerteans.

scholarly journals Physiological and transcriptomic analysis provide novel insight into cobalt stress responses in willow

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yi-Ming Wang ◽  
Qi Yang ◽  
Hui Xu ◽  
Yan-Jing Liu ◽  
Hai-Ling Yang
Keyword(s):  
Stress Responses ◽  
Transcriptomic Analysis ◽  
Insight Into

scholarly journals Ionomic and transcriptomic analysis provides new insight into the distribution and transport of cadmium and arsenic in rice

2017 ◽  
Vol 331 ◽  
pp. 246-256 ◽  
Author(s):  
Xuemin Feng ◽  
Lei Han ◽  
Daiyin Chao ◽  
Yan Liu ◽  
Yajing Zhang ◽  
...  
Keyword(s):  
Transcriptomic Analysis ◽  
Insight Into

scholarly journals Transcriptomic Analysis of the Pistacia vera (L.) Fruits Enable the Identification of Genes and Hormone-Related Gene Linked to Inflorescence Bud Abscission

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 60
Author(s):  
Jubina Benny ◽  
Antonio Giovino ◽  
Francesco Paolo Marra ◽  
Bipin Balan ◽  
Federico Martinelli ◽  
...  
Keyword(s):  
Transcription Factors ◽  
De Novo ◽  
Sugar Metabolism ◽  
Transcriptomic Analysis ◽  
Pistacia Vera ◽  
Alternate Bearing ◽  
Hormone Biosynthesis ◽  
The Individual ◽  
Axillary Inflorescence ◽  
Insight Into

Pistacia vera (L.) is an alternate bearing species. The tree produces axillary inflorescence buds every year. Still, they abscise in “ON” overloaded shoots, causing a limited production in the following “OFF” year, causing a significant and unfavorable production fluctuation. In this work, we carried out de novo discovery and transcriptomic analysis in fruits of “ON” and “OFF” shoots of the cultivar Bianca. We also investigated whether the fruit signaling pathway and hormone biosynthesis directly or indirectly linked to the premature fall of the inflorescence buds causing alternate bearing. We identified 1536 differentially expressed genes (DEGs) in fruits of “ON” vs. “OFF” shoots, which are involved primarily in sugar metabolism, plant hormone pathways and transcription factors. The premature bud abscission linked to the phenomenon is attributable to a lack of nutrients (primarily sugar) and the possible competition between the same branches’ sinks (fruits vs. inflorescence buds). Hormone pathways are involved as a response to signals degradation and remobilization of carbon and nutrients due to the strengthening of the developing embryos. Genes of the secondary metabolism and transcription factors are also involved in tailoring the individual branches response to the nutritional stress and sink competition. Crosstalk among sugar and various hormone-related genes, e.g., ethylene, auxin, ABA and cytokinin, were determined. The discovery of putative biomarkers like callose synthase 5, trehalose-6-phosphate synthase, NAD(P)-linked oxidoreductase and MIOX2, Jasmonate, and salicylic acid-related genes can help to design precision farming practices to mitigate the alternate bearing phenomenon to increase farming profitability. The aim of the analysis is to provide insight into the gene expression profiling of the fate of “ON” and “OFF” fruits associated with the alternate bearing in the pistachio.

scholarly journals Comparative Transcriptomic Analysis Provides Insight into the Domestication and Improvement of Pear (P. pyrifolia) Fruit

2019 ◽  
Vol 180 (1) ◽  
pp. 435-452 ◽  
Author(s):  
Xiaolong Li ◽  
Lun Liu ◽  
Meiling Ming ◽  
Hongju Hu ◽  
Mingyue Zhang ◽  
...  
Keyword(s):  
Transcriptomic Analysis ◽  
Insight Into ◽  
Comparative Transcriptomic Analysis

scholarly journals Healthy Mitochondria: The Brake of Cell Proliferation of Malignant Melanoma

2020 ◽  
Author(s):  
Zhenyao Yu ◽  
Zizhen Zhao ◽  
Chen Fu ◽  
Wei Zhou ◽  
Junchen Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcriptomic Analysis ◽  
Male Mice ◽  
Specific Mechanism ◽  
Male And Female ◽  
Cycle Arrest ◽  
Male Patients ◽  
Anti Tumor Activity ◽  
Insight Into ◽  
Transplantation Therapy

Abstract Background: Melanoma has become the leading cause of death from skin disease, and male patients show higher mortality rate than female. Mitochondrial transplantation therapy is an active area of current research but the anti-melanoma activity and specific mechanism involved in the therapy remain to be fully characterized.Methods: In the study, we intravenously administrated mitochondria extracted from male and female mouse livers respectively to the mice bearing malignantly subcutaneous and metastatic melanoma, and identified the signal mechanism responsible for the mitochondrial treatment through transcriptomic analysis. Meanwhile, the efficiency of female mitochondria and male mitochondria was compared in the cultured melanoma cells and transplanted melanoma in mice. Results: The results suggested that the mitochondria significantly inhibited the tumor growth, and the transcriptomic analysis suggested that general chromosome silencing and local opening of UPRmt effect region were strongly associated with the mitochondria against melanoma., which represented cell cycle arrest, autophagy, and cell apoptosis in the mitochondrial therapy on the metastasis melanoma. Moreover, the anti-tumor activity of mitochondria from female animals was more efficient in comparison to the males, and the female mitochondria could probably induce more persuasive mitochondria-nuclear communication than the mitochondria from male mice.Conclusions: The study not only reveals the anti-tumor mechanism of the mitochondria but also provides a novel insight into the effect of mitochondria in different genders.

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