Emission of volatile esters and transcription of ethylene- and aroma-related genes during ripening of ‘Pingxiangli’ pear fruit (Pyrus ussuriensis Maxim)

2014 ◽  
Vol 170 ◽  
pp. 17-23 ◽  
Author(s):  
Guopeng Li ◽  
Huijuan Jia ◽  
Jihua Li ◽  
Qiang Wang ◽  
Maojun Zhang ◽  
...  
Keyword(s):  
Pear Fruit ◽  
Pyrus Ussuriensis Maxim ◽  
Volatile Esters

Effects of abscisic acid (ABA) on ethanol fermentation during postharvest ripening of Nanguo pear fruit (Pyrus ussuriensis Maxim.)

2021 ◽  
Vol 288 ◽  
pp. 110388
Author(s):  
Jingyi Lv ◽  
Dongle Xu ◽  
Yingzhi Zhang ◽  
Siyang Ding ◽  
Mingyu Sun ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Ethanol Fermentation ◽  
Pear Fruit ◽  
Postharvest Ripening ◽  
Pyrus Ussuriensis Maxim

scholarly journals Rapid Detection and Quantification of Patulin and Citrinin Contamination in Fruits

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4545
Author(s):  
Sudharsan Sadhasivam ◽  
Omer Barda ◽  
Varda Zakin ◽  
Ram Reifen ◽  
Edward Sionov
Keyword(s):  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Pear Fruit ◽  
Cost Effective Method ◽  
Fruit Samples ◽  
Pome Fruits ◽  
Reliable Methods ◽  
By Products ◽  
Detection And Quantification

Patulin (PAT) and citrinin (CTN) are the most common mycotoxins produced by Penicillium and Aspergillus species and are often associated with fruits and fruit by-products. Hence, simple and reliable methods for monitoring these toxins in foodstuffs are required for regular quality assessment. In this study, we aimed to establish a cost-effective method for detection and quantification of PAT and CTN in pome fruits, such as apples and pears, using high-performance liquid chromatography (HPLC) coupled with spectroscopic detectors without the need for any clean-up steps. The method showed good performance in the analysis of these mycotoxins in apple and pear fruit samples with recovery ranges of 55–97% for PAT and 84–101% for CTN, respectively. The limits of detection (LOD) of PAT and CTN in fruits were 0.006 µg/g and 0.001 µg/g, while their limits of quantification (LOQ) were 0.018 µg/g and 0.003 µg/g, respectively. The present findings indicate that the newly developed HPLC method provides rapid and accurate detection of PAT and CTN in fruits.

scholarly journals Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 439
Author(s):  
Naila Boby ◽  
Muhammad Aleem Abbas ◽  
Eon-Bee Lee ◽  
Zi-Eum Im ◽  
Walter H. Hsu ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Adenosine Monophosphate ◽  
Ethanol Ingestion ◽  
Dependent Manner ◽  
Protective Effects ◽  
Gastric Mucosal Lesions ◽  
Cellular Degeneration ◽  
Mucosal Lesions ◽  
Pyrus Ussuriensis Maxim

Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E2 (PGE2), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC50 values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (P < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE2 levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H+/K+ ATPase pathway as well as muscarinic receptor (M3R) and histamine receptor (H2R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK2R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study.

scholarly journals The MIR-Domain of PbbHLH2 Is Involved in Regulation of the Anthocyanin Biosynthetic Pathway in ”Red Zaosu” (PyrusBretschneideri Rehd.) Pear Fruit

2021 ◽  
Vol 22 (6) ◽  
pp. 3026
Author(s):  
Xieyu Li ◽  
Fangxin Xiang ◽  
Wei Han ◽  
Bingqing Qie ◽  
Rui Zhai ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Anthocyanin Biosynthesis ◽  
Bimolecular Fluorescence Complementation ◽  
Anthocyanin Content ◽  
Pear Fruit ◽  
Fruit Peel ◽  
Interaction Domain ◽  
Dihydroflavonol Reductase ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

The N-terminal of Myc-like basic helix-loop-helix transcription factors (bHLH TFs) contains an interaction domain, namely the MYB-interacting region (MIR), which interacts with the R2R3-MYB proteins to regulate genes involved in the anthocyanin biosynthetic pathway. However, the functions of MIR-domain bHLHs in this pathway are not fully understood. In this study, PbbHLH2 containing the MIR-domain was identified and its function investigated. The overexpression of PbbHLH2 in ”Zaosu” pear peel increased the anthocyanin content and the expression levels of late biosynthetic genes. Bimolecular fluorescence complementation showed that PbbHLH2 interacted with R2R3-MYB TFs PbMYB9, 10, and 10b in onion epidermal cells and confirmed that MIR-domain plays important roles in the interaction between the MIR-domain bHLH and R2R3-MYB TFs. Moreover, PbbHLH2 bound and activated the dihydroflavonol reductase promoter in yeast one-hybrid (Y1H) and dual-luciferase assays. Taken together these results suggested that the MIR domain of PbbHLH2 regulated anthocyanin biosynthesis in pear fruit peel.

Simultaneous measurement of oxygen and carbon dioxide diffusivity in pear fruit tissue

2003 ◽  
Vol 29 (2) ◽  
pp. 155-166 ◽  
Author(s):  
W Schotsmans ◽  
B.E Verlinden ◽  
J Lammertyn ◽  
B.M Nicolaı̈
Keyword(s):  
Carbon Dioxide ◽  
Simultaneous Measurement ◽  
Pear Fruit ◽  
Fruit Tissue

Pilot-scale production of cloudy juice from low-quality pear fruit under low-oxygen conditions

2015 ◽  
Vol 173 ◽  
pp. 827-837 ◽  
Author(s):  
Domien De Paepe ◽  
Katleen Coudijzer ◽  
Bart Noten ◽  
Dirk Valkenborg ◽  
Kelly Servaes ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Scale Production ◽  
Low Oxygen ◽  
Pear Fruit ◽  
Pilot Scale Production ◽  
Oxygen Conditions

Purification and characterization of two soluble acid invertase isozymes from Japanese pear fruit

2003 ◽  
Vol 63 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Hiroshi Hashizume ◽  
Koji Tanase ◽  
Katsuhiro Shiratake ◽  
Hitoshi Mori ◽  
Shohei Yamaki
Keyword(s):  
Acid Invertase ◽  
Japanese Pear ◽  
Pear Fruit ◽  
Purification And Characterization ◽  
Soluble Acid Invertase ◽  
Soluble Acid

scholarly journals Effect of High Temperature on Calcium Uptake by Suspension-Cultured Pear Fruit Cells

1987 ◽  
Vol 84 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Joshua D. Klein ◽  
Ian B. Ferguson
Keyword(s):  
High Temperature ◽  
Calcium Uptake ◽  
Pear Fruit
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