Effect of sucrose and mannitol on the accumulation of health-promoting compounds and the activity of metabolic enzymes in broccoli sprouts

2011 ◽  
Vol 128 (3) ◽  
pp. 159-165 ◽  
Author(s):  
Rongfang Guo ◽  
Gaofeng Yuan ◽  
Qiaomei Wang
Keyword(s):  
Metabolic Enzymes ◽  
Broccoli Sprouts ◽  
Health Promoting

Effect of 2, 4-epibrassinolide on main health-promoting compounds in broccoli sprouts

LWT ◽  
2014 ◽  
Vol 58 (1) ◽  
pp. 287-292 ◽  
Author(s):  
Rongfang Guo ◽  
Qiumei Hou ◽  
Gaofeng Yuan ◽  
Yanting Zhao ◽  
Qiaomei Wang
Keyword(s):  
Broccoli Sprouts ◽  
Health Promoting

scholarly journals Sulforaphane from Cruciferous Vegetables: Recent Advances to Improve Glioblastoma Treatment

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1755 ◽  
Author(s):  
Giulia Sita ◽  
Patrizia Hrelia ◽  
Agnese Graziosi ◽  
Fabiana Morroni
Keyword(s):  
Median Overall Survival ◽  
Therapeutic Strategies ◽  
Cruciferous Vegetables ◽  
Cellular Mechanisms ◽  
Broccoli Sprouts ◽  
Leading Role ◽  
Incurable Disease ◽  
Health Promoting ◽  
Low Toxicity ◽  
Poor Efficacy

Sulforaphane (SFN), an isothiocyanate (ITC) derived from cruciferous vegetables, particularly broccoli and broccoli sprouts, has been widely investigated due to its promising health-promoting properties in disease, and low toxicity in normal tissue. Although not yet fully understood, many mechanisms of anticancer activity at each step of cancer development have been attributed to this ITC. Given the promising data available regarding SFN, this review aimed to provide an overview on the potential activities of SFN related to the cellular mechanisms involved in glioblastoma (GBM) progression. GBM is the most frequent malignant brain tumor among adults and is currently an incurable disease due mostly to its highly invasive phenotype, and the poor efficacy of the available therapies. Despite all efforts, the median overall survival of GBM patients remains approximately 1.5 years under therapy. Therefore, there is an urgent need to provide support for translating the progress in understanding the molecular background of GBM into more complex, but promising therapeutic strategies, in which SFN may find a leading role.

Influence of light on health-promoting phytochemicals of broccoli sprouts

2008 ◽  
Vol 88 (5) ◽  
pp. 904-910 ◽  
Author(s):  
Santiago Pérez-Balibrea ◽  
Diego A Moreno ◽  
Cristina García-Viguera
Keyword(s):  
Broccoli Sprouts ◽  
Health Promoting

Effect of Se treatment on glucosinolate metabolism and health-promoting compounds in the broccoli sprouts of three cultivars

2016 ◽  
Vol 190 ◽  
pp. 374-380 ◽  
Author(s):  
Ming Tian ◽  
Xiaoyun Xu ◽  
Yanlong Liu ◽  
Lin Xie ◽  
Siyi Pan
Keyword(s):  
Broccoli Sprouts ◽  
Health Promoting ◽  
Glucosinolate Metabolism

scholarly journals Effect of jasmonic acid on glucosinolate metabolism in different organs of broccoli sprouts

2019 ◽  
pp. 81
Author(s):  
Yinglian Zhu, Fengwu Wang, Liping Guo
Keyword(s):  
Jasmonic Acid ◽  
Cancer Prevention ◽  
Broccoli Sprouts ◽  
Health Promoting ◽  
Glucosinolate Metabolism ◽  
Myrosinase Activity

Edible sprouts, especially Brassica sprouts, contain high levels of health-promoting compounds. Exogenous elicitors have been used as strategies to improve the nutraceutical quality of Brassica sprouts. In this study, effects of jasmonic acid (JA) treatment on growth, the levels of glucosinolates and isothiocyanates, as well as myrosinase activity in different organs of broccoli sprouts were investigated. JA treatment markedly increased the contents of glucosinolates (GSLs), especially glucoraphanin, glucobrassicin and neoglucobrassicin in broccoli sprouts. However, gluconapin was not affected even decreased by JA treatment. Cotyledon, hypocotyl and root obtained the different results in induction of GSLs. Among these, neoglucobrassicin obtained the highest enhancement in three organs. Myrosinase activity in cotyledon of broccoli increased after JA treatment, while decreased in hypocotyl. Three concentrations of JA all significantly increased sulforaphane and isothiocyanates formation in cotyledon, hypocotyl and root of broccoli sprouts. Application of 100 μM JA led to the highest myrosinase activity, the least gluconapin and the most sulforaphane and isothiocyanates in cotyledon, as well as the most isothiocyanates in root. These results indicated that JA treatment could be an effective way to improve the cancer-prevention benefits of broccoli sprouts via enhancing sulforaphane and total isothiocyanates.

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

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