In vitro plantlets from alginate-encapsulated shoot tips of Solanum nigrum L.

2010 ◽  
Vol 124 (4) ◽  
pp. 517-521 ◽  
Author(s):  
Satish K. Verma ◽  
Manoj K. Rai ◽  
Pooja Asthana ◽  
V.S. Jaiswal ◽  
U. Jaiswal
Keyword(s):  
Solanum Nigrum ◽  
Shoot Tips ◽  
In Vitro Plantlets ◽  
Solanum Nigrum L

scholarly journals Antioxidant Activities of Solanum Nigrum L. Leaf Extracts Determined in in vitro Cellular Models

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 63 ◽  
Author(s):  
Agata Campisi ◽  
Rosaria Acquaviva ◽  
Giuseppina Raciti ◽  
Anna Duro ◽  
Milena Rizzo ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Cell Damage ◽  
Glutamate Uptake ◽  
Primary Cultures ◽  
Solanum Nigrum ◽  
Oxidative Status ◽  
Glutamate Excitotoxicity ◽  
Cellular Models ◽  
Solanum Nigrum L

Several medicinal foods abound in traditional medicine with antioxidant potentials that could be of importance for the management of several diseases but with little or no scientific justification to substantiate their use. Thus, the objective of this study was the assessment of the antioxidant effect of two leave extracts of Solanum nigrum L. (SN), which is a medicinal plant member of the Solanaceae family, mainly used for soup preparation in different parts of the world. Then methanolic/water (80:20) (SN1) and water (SN2) leaves extracts were prepared. The total polyphenolic content and the concentration of phenolic acids and flavones compounds were determined. In order to verify whether examined extracts were able to restore the oxidative status, modified by glutamate in primary cultures of astrocytes, the study evaluated the glutathione levels, the intracellular oxidative stress, and the cytotoxicity of SN1 and SN2 extracts. Both extracts were able to quench the radical in an in vitro free cellular system and restore the oxidative status in in vitro primary cultures of rat astroglial cells exposed to glutamate. These extracts prevented the increase in glutamate uptake and inhibited glutamate excitotoxicity, which leads to cell damage and shows a notable antioxidant property.

scholarly journals Manipulating Some Culturing Conditions Enhances Production of Solanine in Microshoots, Callus and Cell suspension Cultures of Solanum nigrum L.: A Wild Medicinal Plant

2020 ◽  
Vol 24 ◽  
pp. 51
Author(s):  
Rida A Shibli
Keyword(s):  
Light Intensity ◽  
Medicinal Plant ◽  
Cell Suspension ◽  
Growth Parameters ◽  
Cell Suspension Cultures ◽  
Solanum Nigrum ◽  
Callus Growth ◽  
Culture Techniques ◽  
Solanum Nigrum L

Solanum nigrum L. is a medicinal plant of solanaceae family with distinguished therapeutic properties. Traditionally, S. nigrum. had been used as an anti-tumorgenic, antioxidant, hepatoprotective, diuretic, and antipyretic agent. The most important alkaloid member in this plant is solanine. Therefore, this study was conducted to utilize tissue culture techniques for the enhancement of solanine production in the in vitro grown cultures of this promising neglected plant. For callus growth and development experimental part, the highest callus growth parameters (callus diameter (21.4 mm) and callus fresh weight (2202.4 mg) were obtained in callus grown on Murashige & Skoog MS media supplemented with 2,4-Dichlorophenoxyacetic acid (2.0 mg·L-1) plus 1.5 mg·L-1 Thidiazuron. Similar trend was also obtained in cell suspension culture experiment, as maximum growth was recorded at similar hormone combination. Moreover, High-performance liquid chromatography analysis revealed that, solanine was affected by growth regulator type and concentration. The highest solanine levels were obtained when the explants were treated with 6-benzylaminopurine at level of 2.0 mg·L-1, as solanine content reached up to (2.61, 1.53 mg.g-1) for callus and cell suspension, respectively, while, microshoot contained the highest solanine (4.52 mg.g-1 DW) at 6-benzylaminopurine level of 1.6 mg.L-1. Additionally, carbon source had positively affected solanine level, where 0.2 M sucrose resulted in production of the highest amounts (3.13, 2.03 and 1.20 mg.g-1 DW) of solanine in microshoots, callus and cell suspension, respectively. Also, exposing microshoots and callus to light intensity of (100 µmol.m -2 s-1) yielded the highest solanine content (4.03 and 1.26 mg.g-1 DW, respectively),while the lowest solanine levels (1.50 and 0.48 mg.g-1 DW) were observed in plant material exposed to the lowest light intensity treatment (25 µmol.m -2 s-1) .

scholarly journals Preservation of In Vitro Grown Shoot Tips of Potato (Solanum tuberosum L.) by Different Methods of Cryopreservation

1970 ◽  
Vol 10 ◽  
pp. 15-20
Author(s):  
Shambhu P. Dhital ◽  
Hira K. Manandhar ◽  
Hak T. Lim
Keyword(s):  
Sucrose Concentration ◽  
Shoot Tips ◽  
Efficient Tool ◽  
Long Term Storage ◽  
In Vitro Plantlets ◽  
Tuber Morphology ◽  
Vegetatively Propagated Plants ◽  
Term Storage

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, Atlantic and Superior were cryopreserved by vitrification, encapsulationvitrification and encapsulation-dehydration. Cryopreservation by vitrification method was used to determine the optimum concentration of sucrose and cold hardening temperature during sub-culturing period to the donor plantlets. Nine-percent sucrose gave 46.7% survival in Atlantic and 40% in Superior. The most optimum hardening temperature for 50% survival in Atlantic and 43.3% in Superior was 10°C. In the case of comparative study of three different cryopreservation methods, the highest survival (52%) as well as regeneration (46%) were observed when the shoot tips were cryopreserved by encapsulation-vitrification method, and the lowest survival (36%) and regeneration (28%) from the vitrification. Plant and tuber morphology of potato regenerated after cryopreservation were similar to those of the non-cryopreserved in vitro plantlets (control). Thus, this study demonstrated that encapsulation-vitrification method was the most effective one among other methods for higher survival as well as regeneration in in vitro shoot tips of potato.Key words: Cryopreservation; Dehydration; Encapsulation; Potato; Regeneration; VitrificationDOI: 10.3126/njst.v10i0.2804Nepal Journal of Science and Technology Volume 10, 2009 December Page: 15-20

scholarly journals Effect of chemical elicitors on secondary metabolite induction of Solanum nigrum In vitro.

2014 ◽  
Vol 8 (3) ◽  
pp. 28-34
Author(s):  
Baan M. Abdulrazzak ◽  
Saadia H. Mahmood ◽  
Kadhim M. Ibrahim
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Jasmonic Acid ◽  
Callus Cultures ◽  
Solanum Nigrum ◽  
Naphthalene Acetic Acid ◽  
Leaf Discs ◽  
Methanolic Extracts ◽  
Solanum Nigrum L

This project aimed to increase the production of some secondary metabolites using chemical elicitors in tissue cultures of Solanum nigrum L. plants. The quality and quantity of phytochemicals were estimated using methanolic extracts of dried leaves and callus were analyzed using HPLC. Callus was initiated from leaf discs cultured on Murashig and Skoog (MS) medium supplemented with Naphthalene acetic acid (NAA) at concentrations of 1.0, 2.0, 3.0, 4.0 or 5.0 mg/l and BA at 0.5, 1.0, 1.5, 2.0 or 2.5 mg/l for S. nigrum callus initiation. The same combination was used for callus maintenance for the plant. The results showed an increase in the concentration of secondary metabolites in methanol extracts induced from leaves. Callus cultures induced from leaf discs were treated with some chemical stimuli such as jasmonic acid and salicylic acid, (2,4,6, 8 mg/l jasmonic acid), or (50, 100, 150, 200 mg/l salicylic acid). Result showed that there were significant differences between the various treatment, the best concentration of jasmonic acid to stimulate and increase the production was 8 mg/l for S. nigrum. Moreover, Salicylic acid at 100 mg/l stimulated the production of secondary metabolites in callus culture of S. nigrum.

scholarly journals In vitro Regeneration and Flower Induction on Solanum nigrum L. from Pachamalai hills of Eastern Ghats

1970 ◽  
Vol 18 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Amzad Basha Kolar ◽  
L . Vivekanandan ◽  
Ghouse Basha M
Keyword(s):  
In Vitro Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Solanum Nigrum ◽  
In Vitro Flowering ◽  
Eastern Ghats ◽  
Solanum Nigrum L

 Explants of Solanum nigrum L., collected from Pachamalai hills callused successfully on MS basal medium supplemented with IAA and BAP. The highest frequency of green compact callus and multiple shoots were obtained on MS containing 2.0 mg/l IAA and 0.5 mg/l BAP. The callus when cultured on MS basal medium fortified with different concentrations of BAP (3.0 - 8.0 mg/l) and IAA (0.5 mg/l) showed multiple shoot formation. The highest frequency of multiple shoots was obtained on MS containing 6.0 mg/l BAP and 0.5 mg/l IAA. For in vitro flowering, the node explants were cultured on MS fortified with different concentrations of BAP (2.0 - 7.0 mg/l) and NAA (0.5 mg/l). The highest number of multiple shoots were obtained in MS supplemented with 6.0 mg/l BAP and 0.5 mg/l NAA. The in vitro flowering was observed on MS containing 2,4-D and BAP 1.5 mg/l, respectively. The best rooting was obtained on MS containing 0.5 mg/l IBA. The well-rooted plants were hardened and finally planted in the garden.  Key words: In vitro studies, Medicinal plant, Solanum nigrum, Node, Callus D.O.I. 10.3329/ptcb.v18i1.3264 Plant Tissue Cult. & Biotech. 18(1): 43-48, 2008 (June)

Photosynthetic and transpiration responses of in vitro-regenerated Solanum nigrum L. plants to ex vitro adaptation

2010 ◽  
Vol 46 (2) ◽  
pp. 134-141 ◽  
Author(s):  
Swasti S. Swain ◽  
Tapasi Tripathy ◽  
Pradipta K. Mohapatra ◽  
Pradeep K. Chand
Keyword(s):  
Solanum Nigrum ◽  
Ex Vitro ◽  
Solanum Nigrum L

scholarly journals In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

2017 ◽  
Vol 7 (3) ◽  
pp. 169-180 ◽  
Author(s):  
Hanan Abd Al-Hay Saied Al-Ashaal ◽  
◽  
Hanan Farouk Aly Abduallah ◽  
Ayman Ali Farghaly ◽  
Sanaa Ahmed Ali ◽  
...  
Keyword(s):  
Potential Role ◽  
Solanum Nigrum ◽  
Solanum Nigrum L
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