Resistance to freezing in liquid nitrogen of carnation (Dianthus caryophyllus L. var Eolo) apical and axillary shoot tips excised from different aged in vitro plantlets

1988 ◽  
Vol 7 (3) ◽  
pp. 170-173 ◽  
Author(s):  
J. Dereuddre ◽  
J. Fabre ◽  
C. Bassaglia
Keyword(s):  
Liquid Nitrogen ◽  
Dianthus Caryophyllus ◽  
Shoot Tips ◽  
Axillary Shoot ◽  
In Vitro Plantlets

scholarly journals In vitro Conservation and Cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub

2013 ◽  
Vol 41 (2) ◽  
pp. 638 ◽  
Author(s):  
Aylin OZUDOGRU ◽  
Diogo Pedrosa Corrêa Da SILVA ◽  
Ergun KAYA ◽  
Giuliano DRADI ◽  
Renato PAIVA ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Storage Temperature ◽  
Sucrose Concentration ◽  
Aluminum Foil ◽  
Shoot Tips ◽  
In Vitro Conservation ◽  
Shoot Cultures ◽  
Storage Medium ◽  
Droplet Vitrification

The study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.

scholarly journals Preservation of In Vitro Grown Shoot Tips of Potato (Solanum tuberosum L.) by Different Methods of Cryopreservation

1970 ◽  
Vol 10 ◽  
pp. 15-20
Author(s):  
Shambhu P. Dhital ◽  
Hira K. Manandhar ◽  
Hak T. Lim
Keyword(s):  
Sucrose Concentration ◽  
Shoot Tips ◽  
Efficient Tool ◽  
Long Term Storage ◽  
In Vitro Plantlets ◽  
Tuber Morphology ◽  
Vegetatively Propagated Plants ◽  
Term Storage

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, Atlantic and Superior were cryopreserved by vitrification, encapsulationvitrification and encapsulation-dehydration. Cryopreservation by vitrification method was used to determine the optimum concentration of sucrose and cold hardening temperature during sub-culturing period to the donor plantlets. Nine-percent sucrose gave 46.7% survival in Atlantic and 40% in Superior. The most optimum hardening temperature for 50% survival in Atlantic and 43.3% in Superior was 10°C. In the case of comparative study of three different cryopreservation methods, the highest survival (52%) as well as regeneration (46%) were observed when the shoot tips were cryopreserved by encapsulation-vitrification method, and the lowest survival (36%) and regeneration (28%) from the vitrification. Plant and tuber morphology of potato regenerated after cryopreservation were similar to those of the non-cryopreserved in vitro plantlets (control). Thus, this study demonstrated that encapsulation-vitrification method was the most effective one among other methods for higher survival as well as regeneration in in vitro shoot tips of potato.Key words: Cryopreservation; Dehydration; Encapsulation; Potato; Regeneration; VitrificationDOI: 10.3126/njst.v10i0.2804Nepal Journal of Science and Technology Volume 10, 2009 December Page: 15-20

In vitro plantlets from alginate-encapsulated shoot tips of Solanum nigrum L.

2010 ◽  
Vol 124 (4) ◽  
pp. 517-521 ◽  
Author(s):  
Satish K. Verma ◽  
Manoj K. Rai ◽  
Pooja Asthana ◽  
V.S. Jaiswal ◽  
U. Jaiswal
Keyword(s):  
Solanum Nigrum ◽  
Shoot Tips ◽  
In Vitro Plantlets ◽  
Solanum Nigrum L

scholarly journals Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification

2017 ◽  
Vol 66 (1-2) ◽  
pp. 44-50
Author(s):  
Tatjana Vujović ◽  
Đurđina Ružić ◽  
Radosav Cerović
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Shoot Tips ◽  
Lower Survival Rate ◽  
Droplet Vitrification ◽  
Vitrification Solution ◽  
Long Term Preservation ◽  
Direct Immersion

SummaryIn vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material.

Cold hardening and slow cooling: tools for successful cryopreservation and recovery of in vitro shoot tips of silver birch

1996 ◽  
Vol 26 (11) ◽  
pp. 2015-2022 ◽  
Author(s):  
Leena Ryynänen
Keyword(s):  
Dimethyl Sulfoxide ◽  
Liquid Nitrogen ◽  
Callus Formation ◽  
Shoot Tips ◽  
Silver Birch ◽  
Mature Trees ◽  
Slow Cooling ◽  
Freezing Procedure ◽  
Light:Dark Cycle

Shoot tips of silver birch (Betulapendula Roth) derived from in vitro cultures originating from mature trees were used as explant material. Several pretreatments prior to cryopreservation were studied in order to enhance shoot tip recovery after storage in liquid nitrogen. Cold acclimation and the classical slow freezing procedure proved to be essential for successful survival, while both nonhardening and vitrification resulted in minimal survival. The optimum procedure was the following: Shoots were cold hardened (5 °C on a 8 h: 16 h (light:dark) cycle) for 3 weeks, and dissected shoot tips were then precultivated on woody plant medium with 5% dimethyl sulfoxide for 72 h. The material was transferred to cryotubes, and a mixture of 10% polyethylene glycol, 10% glucose, and 10% dimethyl sulfoxide was used as cryoprotectant. The cryotubes were slowly cooled to −38 °C before immersion in liquid nitrogen for 8 d. After fast thawing, axillary buds formed shoots without callus formation, but the growth of the new shoots was delayed from a few days to several weeks. Variability in regrowth was considerable as a result of genotype differences.

scholarly journals Development of the first axillary in vitro shoot multiplication protocol for coconut palms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hannes Wilms ◽  
Dries De Bièvre ◽  
Kevin Longin ◽  
Rony Swennen ◽  
Juhee Rhee ◽  
...  
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Pests And Diseases ◽  
Planting Material ◽  
In Vitro Plantlets ◽  
Disease Free

AbstractThe coconut palm or “tree of life” is one of nature’s most useful plants and the demand for its fruit is increasing. However, coconut production is threatened by ageing plantations, pests and diseases. Currently, the palm is exclusively propagated via seeds, limiting the amount of planting material. A novel micropropagation method is presented, based on axillary shoot formation. Apical meristems of in vitro coconut seedlings are cultured onto Y3 medium containing 1 µM TDZ. This induces the apical meristem to proliferate through axillary shoots in ~ 27% of the initiated explants. These axillary shoots are seen as white clumps of proliferating tissue and can be multiplied at a large scale or regenerated into rooted in vitro plantlets. This innovative micropropagation method will enable the production of disease-free, high quality in vitro plantlets, which will solve the worldwide scarcity of coconut planting material.

scholarly journals In vitro Axillary Shoot Regeneration and Direct Protocorm-like Body Induction from Axenic Shoot Tips of Doritis pulcherrima Lindl.

2014 ◽  
Vol 23 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Tustu Mondal ◽  
Sumana Aditya ◽  
Nirmalya Banerjee
Keyword(s):  
Root Formation ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Tree Bark ◽  
Axillary Shoot ◽  
Inhibitory Effects ◽  
Terrestrial Orchid ◽  
Root Regeneration ◽  
Shoot Tip Explants

An efficient protocol for in vitro propagation of an important ornamental terrestrial orchid, Doritis pulcherima Lindl. through axillary shoot and direct protocorm-like body (PLB) formation from shoot tip explants derived from six- month-old axenic seedlings has been described. Shoot tips were cultured on modified nutrient medium of Knudson’s C supplemented with 0.1% peptone and combination of various concentrations of NAA and BAP. The effect of NAA and BAP on axillary shoot formation, protocorm-like body induction and root regeneration from the explants was significant. The highest frequency of axillary shoot formation was recorded in the medium containing 2 mg/l BAP and the PLB production was higher in the medium containing 2 mg/l NAA. A higher concentration of BAP showed inhibitory effects on the axillary shoot formation and PLB induction. Efficient root regeneration was observed in low concentration of NAA. However, the profuse root formation was common in the PGR free medium. Rooted plantlets were hardened successfully through the stepwise acclimation protocol and platelets were finally established in the potting mixture containing small pieces of dead tree bark of mango, charcoal pieces and broken bricks in 1 : 2 : 1 ratio. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17526 Plant Tissue Cult. & Biotech. 23(2): 251-261, 2013  (December)

scholarly journals Cryopreservation and Acclimatization of Lycopersicon esculentum (Mill.) Genotypes

2014 ◽  
Vol 42 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Ana COSTE ◽  
Sergiu VALIMAREANU ◽  
Adela HALMAGYI
Keyword(s):  
Lycopersicon Esculentum ◽  
Liquid Nitrogen ◽  
Growth Conditions ◽  
Alginate Beads ◽  
Shoot Tips ◽  
Ex Vitro ◽  
Fresh Weight Basis ◽  
Ex Vitro Growth ◽  
Laminar Air Flow

Romanian tomato (Lycopersicon esculentum Mill.) cultivars have been cryopreserved by encapsulation-dehydration and successfully acclimatized to ex vitro growth conditions. Shoot tips were excised from in vitro grown plants then precultured for 24 h in various sucrose concentrations, dehydrated up to 6 h in laminar air flow prior to direct immersion in liquid nitrogen   (−196°C) for 24 h. Different parameters have been studied: the effects of osmoprotection and desiccation duration on the regrowth of cryopreserved shoot tips, the effects of various IBA concentrations on rooting and the ex vitro cclimatization of plants recovered from liquid nitrogen. The highest frequency of regrowth (72% cv. ‘Pontica’) was obtained when encapsulated explants were precultured in 0.5 M sucrose and the moisture content (fresh weight basis) of alginate beads was 23%. The highest rooting rates (58% to 77%) for all cultivars were observed for shoots grown on MS medium supplemented with 1.0 mg/l IBA. The rooted plants could be easily acclimatized ex vitro with up to 100% survival.

scholarly journals Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates

2011 ◽  
Vol 28 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Kentaro Sekizawa ◽  
Shin-ichi Yamamoto ◽  
Tariq Rafique ◽  
Kuniaki Fukui ◽  
Takao Niino
Keyword(s):  
Dianthus Caryophyllus ◽  
Shoot Tips
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