Towards crop improvement in bell pepper (Capsicum annuum L.): Transgenics (uid A::hpt II) by a tissue-culture-independent Agrobacterium-mediated in planta approach

2009 ◽  
Vol 119 (4) ◽  
pp. 362-370 ◽  
Author(s):  
A. Manoj Kumar ◽  
Kalpana N. Reddy ◽  
Rohini Sreevathsa ◽  
Girija Ganeshan ◽  
M. Udayakumar
Keyword(s):  
Tissue Culture ◽  
Capsicum Annuum ◽  
Crop Improvement ◽  
Bell Pepper ◽  
In Planta ◽  
Capsicum Annuum L ◽  
Culture Independent

scholarly journals In-planta Agrobacterium-mediated Stable hva1 and EPSPS Integration into Potato var. Agria Genome

2021 ◽  
Author(s):  
Darush Choobineh ◽  
nafiseh mahdi nezhad ◽  
Ali Niazi Niazi ◽  
Baratali Fakheri ◽  
Abbasali Emamjomeh
Keyword(s):  
Tissue Culture ◽  
Transgene Expression ◽  
Direct Injection ◽  
Crop Improvement ◽  
Constitutive Expression ◽  
Transformation Method ◽  
Bacterial Suspension ◽  
Potato Seed ◽  
In Planta ◽  
Culture Independent

Abstract BackgroundAfter wheat, maize, and rice, potato is not only an important food crop but also a substantial source of income throughout the world. Developing a practical and effective transformation method for cultivars that are recalcitrant in tissue culture is vital. Hva1 encodes the protein of the LEA III superfamily that involves in reactions to abiotic stresses, which holds considerable potential for use as molecular tools for genetic crop improvement toward stress tolerance.ResultsHere, a protocol has been designed for an Agrobacterium-mediated transient transformation in tissue culture-independent conditions in-planta. The protocol establishes for hva1 and EPSPS transformations by direct injection of the bacterial suspension into the potato tuber sprout to encode resistance to cold and against glyphosate herbicide. A two-stage selection was involved using 1% and 2% Glyphosate to eliminate the chimeric and non-transformed plants. Ultimately, the protocol enabled confirmation of gene integration into the plant, transgene expression of the gene and transgene expression, which was made possible by competitive PCR reaction, RT-PCR, and ELISA, respectively. In this research, the transformation efficiencies acquired in potatoes (up to 46%) were higher than those reported using conventional Agrobacterium-mediated approaches in previous studies. ConclusionsThe constitutive expression of the integrated T-DNA neither slowed down the growth rate nor affected potato tuberization significantly. The hva1 gene was expressed successfully leading to the accumulation of the hva1 protein in transgene-generated tubers. This study is the first report on a successful transformation of potato in-planta whereby Agrobacterium can be directed at potato seed sprouts through injection.

scholarly journals TEORES DE MICRONUTRIENTES NO DESENVOLVIMENTO DA PLANTA DE PIMENTÃO SOB FERTIRRIGAÇÃO

Irriga ◽  
2003 ◽  
Vol 8 (2) ◽  
pp. 120-131 ◽  
Author(s):  
Francisco Fernando Noronha Marcussi ◽  
Roberto Lyra Villas Bôas
Keyword(s):  
Capsicum Annuum ◽  
Growth Period ◽  
Bell Pepper ◽  
Soil Science ◽  
Pepper Plant ◽  
Cu Content ◽  
Capsicum Annuum L ◽  
Entire Plant ◽  
Micronutrient Concentration ◽  
E Mail

TEORES DE MICRONUTRIENTES NO DESENVOLVIMENTO DA PLANTA DE PIMENTÃO SOB FERTIRRIGAÇÃO   Francisco Fernando Noronha MarcussiDepartamento de Engenharia Rural, Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected] Lyra Villas BôasDepartament de Recursos Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected]  1 RESUMO  Neste estudo, objetivou-se determinar os teores de micronutrientes (Fe, Zn, Mn, B e Cu) nos diferentes órgãos do híbrido de pimentão Elisa, sob fertirrigação em ambiente protegido, em oito épocas de coleta de planta. O experimento foi desenvolvido nas dependências do Departamento de Recursos Naturais - Ciência do Solo, FCA, Botucatu/SP. Constou o experimento de 8 tratamentos (épocas de coleta de planta – 0, 20, 40, 60, 80, 100, 120, e 140 dias após o transplante das mudas) e 4 repetições, sendo cada repetição formada por 4 plantas. Em cada etapa obteve-se os teores dos micronutrientes no fruto, folha, caule e raiz e na planta como um todo. A fertilização no ciclo estudado, 140 dias, foi feita via fertirrigação localizada. Os resultados mostram que os teores de micronutrientes encontrados na planta de pimentão seguem a seqüência: Fe>Zn>Mn>B>Cu. Nos resultados são apresentadas as tabelas com os teores de Fe, Zn, Mn, B e Cu (Tukey a 5%) em cada órgão da planta e da planta como um todo, nas diferentes épocas de avaliação e, os gráficos mostrando o comportamento dos teores de Zn, Mn, B e Cu.  UNITERMOS: Fertirrigação, concentrações de micronutrientes, Pimentão, Capsicum annuum L. e nutrição mineral.   MACUSSI, F.F.N.; VILLAS BÔAS, R.L MICRONUTRIENT CONCENTRATION IN THE BELL PEPPER PLANT DEVELOPMENT UNDER FERTIRRIGATION   2 ABSTRACT  This study aimed to determine the micronutrient contents (Fe, Zn, Mn, B and Cu) in different organs of Elisa bell pepper hybrid under fertirrigation in protected environment in eight harvesting seasons. The experiment was carried out in the Natural Resource Department – Soil Science, FCA, Botucatu. SP, and  consisted of 8 treatments (harvesting season – 0, 20, 40, 60, 80, 100 and 140 days after seedling transplant), and 4 replications, each one composed of 4 plants. In each growth period the micronutrient content in the fruit, leaf, stem, root and  plant as a whole was determined. The fertilization in the study period i.e., 140 days, was performed by local fertirrigation. The results showed that the micronutrient levels found in the bell pepper plant followed the decreasing order: Fe>Zn>Mn> B>Cu. The tables present the Fe, Zn, Mn, B and Cu (Tukey Test at 5%) contents in each plant organ and in the entire plant at different evaluation periods. The charts show the Zn, Mn, B and Cu content variation.  KEYWORDS: fertirrigation, micronutrient concentration, bell pepper, Capsicum annuum L. and mineral nutrition. 

scholarly journals YIELD AND TOLERANCE OF BELL PEPPER (CAPSICUM ANNUUM L.) CULTIVARS TO POTATO VIRUS Y ISOLATE FROM PUERTO RICO

1969 ◽  
Vol 71 (3) ◽  
pp. 337-340
Author(s):  
Jaime Escudero ◽  
Guillermo Fornaris-Rullán ◽  
Elvin Caraballo
Keyword(s):  
Puerto Rico ◽  
Capsicum Annuum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Bell Pepper ◽  
Capsicum Annuum L

YIELD AND TOLERANCE OF BELL PEPPER (CAPSICUM ANNUUM L.) CULTIVARS TO POTATO VIRUS Y ISOLATE FROM PUERTO RICO

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