Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): Induction, conversion and synthetic seeds

2007 ◽  
Vol 111 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Lírio Luiz Dal Vesco ◽  
Douglas Steinmacher ◽  
Antonio Carlos Torres ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Synthetic Seeds ◽  
Acca Sellowiana

scholarly journals Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

2014 ◽  
Vol 23 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Dinesh Giri ◽  
Sushma Tamta
Keyword(s):  
Somatic Embryogenesis ◽  
Medicinal Plant ◽  
Somatic Embryos ◽  
Synthetic Seeds ◽  
Ex Situ ◽  
Secondary Embryogenesis ◽  
Zygotic Embryos ◽  
High Concentration ◽  
Cotyledon Explants ◽  
Short Period

This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013  (December)

scholarly journals Effects of glutathione supplementation and carbon source during somatic embryogenesis of Acca sellowiana (O.Berg) Burret (Myrtaceae)

2018 ◽  
Vol 40 (1) ◽  
pp. 40257
Author(s):  
Ana Flávia Pavei ◽  
Hugo Pacheco de Freitas Fraga ◽  
Leila Do Nascimento Vieira ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Carbon Source ◽  
Acca Sellowiana

5-Azacytidine combined with 2,4-D improves somatic embryogenesis of Acca sellowiana (O. Berg) Burret by means of changes in global DNA methylation levels

2012 ◽  
Vol 31 (12) ◽  
pp. 2165-2176 ◽  
Author(s):  
Hugo P. F. Fraga ◽  
Leila N. Vieira ◽  
Clarissa A. Caprestano ◽  
Douglas A. Steinmacher ◽  
Gustavo A. Micke ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Somatic Embryogenesis ◽  
Global Dna Methylation ◽  
Acca Sellowiana

SOMATIC EMBRYOGENESIS AND PRODUCTION OF SYNTHETIC SEEDS IN ZIZIPHUS

2013 ◽  
pp. 83-90 ◽  
Author(s):  
C. Sudhersan ◽  
S. Jibimanuel ◽  
J. Ashkanani
Keyword(s):  
Somatic Embryogenesis ◽  
Synthetic Seeds

scholarly journals The role of γ -aminobutyric acid (Gaba) in somatic embryogenesis of Acca sellowiana Berg. (Myrtaceae)

2009 ◽  
Vol 21 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Maristela Raitz Booz ◽  
Gilberto B. Kerbauy ◽  
Miguel Pedro Guerra ◽  
Rosete Pescador
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Somatic Embryos ◽  
Culture Medium ◽  
Aminobutyric Acid ◽  
Zygotic Embryos ◽  
Protein Amino Acid ◽  
Endogenous Gaba ◽  
Acca Sellowiana

The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in a large number of normal embryos, and the lowest percentage of formation of fused somatic embryos, phenotypic characteristic of most deformed embryos in all treatments. Also, all treatments promoted the formation of the somatic embryos with positive characteristics of development resumption, which however did not originate the seedlings.

Dynamics of free and3H-labelled glutamine concentrations during zygotic and somatic embryogenesis of Feijoa [Acca sellowiana(O. Berg.) Burret]

2012 ◽  
Vol 87 (6) ◽  
pp. 583-587 ◽  
Author(s):  
R. Pescador ◽  
G. B. Kerbauy ◽  
H.P.F. Fraga ◽  
R. M. Hamasaki ◽  
L.B.B. Tavares ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Acca Sellowiana

Advances and constraints in somatic embryogenesis of Araucaria angustifolia, Acca sellowiana, and Bactris gasipaes

2020 ◽  
Vol 143 (2) ◽  
pp. 241-263
Author(s):  
Valdir Marcos Stefenon ◽  
Joseph Francis Ree ◽  
Marcos Vinicius Marques Pinheiro ◽  
Daniela Goeten ◽  
Neusa Steiner ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Bactris Gasipaes ◽  
Araucaria Angustifolia ◽  
Acca Sellowiana

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

Biologia ◽  
2014 ◽  
Vol 69 (2) ◽  
Author(s):  
Vijay Kumar ◽  
Sheela Chandra
Keyword(s):  
Somatic Embryogenesis ◽  
Seed Production ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Synthetic Seeds ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Swertia Chirayita

AbstractAn efficient protocol for plant regeneration through somatic embryogenesis was established from in vivo leaf explants of Swertia chirayita, a critically endangered medicinal herb. The highest frequency (76%) of embryogenic callus was induced on Murashige & Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L kinetin (Kn) from in vivo leaf explants. Globular somatic embryos were induced and further matured from such embryogenic calli by subsequent culture on the same medium. The highest number of somatic embryos (48.83 ± 4.6) was recovered from embryogenic calli derived from leaf explants after 6 weeks of culture. Synthetic seeds were produced by encapsulating of torpedo stage embryos in sodium alginate (4% W/V) gel, dropped into 100 mM calcium chloride (CaCl2 · 2H2O) solution. The synthetic seeds were germinated on MS medium. The highest frequency of synthetic seed germination (84%) was observed on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA. Regenerants were successfully acclimatized under ex vitro condition. This is the first report on synthetic seed production of S. chirayita. Application of these protocols would be helpful in reducing stress in natural habitat, and in long-term storage of elite genotypes through synthetic seed production.

scholarly journals Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

2009 ◽  
Vol 44 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Neusa Steiner ◽  
Sara Beatriz Maldonado ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Total Protein ◽  
Somatic Embryos ◽  
Periodic Acid ◽  
Soluble Sugars ◽  
Buffer Solution ◽  
Periodic Acid Schiff ◽  
Total Sugars ◽  
Protein Levels ◽  
Acca Sellowiana

The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana.

Sign in / Sign up

Export Citation Format

Share Document