Assessment of the stability and adaptability of waxbloom and waxless pea (Pisum sativum L.) mutant lines

2006 ◽  
Vol 109 (1) ◽  
pp. 15-20 ◽  
Author(s):  
R. Vassilevska-Ivanova ◽  
N. Naidenova
1983 ◽  
Vol 63 (3) ◽  
pp. 591-599 ◽  
Author(s):  
S. L. A. HOBBS ◽  
J. D. MAHON

Symbiotic nitrogen fixation was examined in 36 plant genotype-bacterial strain combinations produced by growing six genotypes of Pisum sativum L. and six strains of Rhizobium leguminosarum in all combinations. Both genotypes and strains had effects not only on nitrogen fixation but also on characters associated with plant growth and photosynthesis. However, relationships between characters differed markedly depending on whether genotype or strain means were used to calculate correlation coefficients. Genotype × strain (G × S) interactions also affected the expression of several of the characters. Using nitrogen fixation as an example, statistical methods analogous to those developed for the analysis of genotype × environment interactions were used to study this interaction. From this analysis it was apparent that the G × S variability was mainly caused by differences in the magnitude of the response of plant genotypes or bacterial strains to changes in the complementary symbiont with little difference in the stability of this response. An examination of different indicators of performance, response, and stability of that response, suggests that both plant genotypes and bacterial strains could be selected for relatively uniform fixation over a range of symbiotic partners, or that specific combinations could be selected for maximum symbiotic effectiveness.Key words: Genotype × strain, N2 fixation, photosynthesis, respiration, growth


2009 ◽  
Vol 45 (No. 2) ◽  
pp. 57-71 ◽  
Author(s):  
J. Horáček ◽  
M. Griga ◽  
P. Smýkal ◽  
M. Hýbl

Environmental (geographic location, year-to-year effect) and genetic (intravarietal variation) effects on the stability of the isozyme patterns of esterase (EST), acid phosphatase (ACP), alcohol dehydrogenase (ADH), leucine aminopeptidase (LAP), shikimate dehydrogenase (SDH) and glucose-6-phosphate isomerase (PGI) used for pea cultivar genotyping were studied. In addition, selected DNA markers (RAPD, SSR, ISSR, IRAP) were used to study intravarietal genetic homogeneity/variation at a DNA level. Five commercial dry-seed pea (Pisum sativum L.) cultivars Canis, Gotik, Komet, Sonet and Zekon were grown during four years (2001 – 2004) in three locations in the Czech Republic (Šumperk, Čáslav, Uherský Ostroh) that differed in soil and climatic characteristics. Mature dry seeds were used as a standard input sample for analyses. No variation in isozyme spectra was found within particular cultivars between years, locations and fruiting nodes in enzymes providing in general a low polymorphism in pea cultivars (ACP, ADH, LAP, SDH, PGI); similarly, these enzymes also exhibited high stability as related to intravarietal variation. In contrast, EST – highly polymorphic in pea cultivars – showed certain qualitative variation within particular cultivars as related to both environmental and genetic factors. The intravarietal variation detected by selected DNA markers was negligible and mostly quantitative. Possible reasons for the instability/variation of isozyme markers are discussed from the aspect of cultivar genotyping used in pea breeding and seed production.


2012 ◽  
Vol 10 (4) ◽  
pp. 50-55
Author(s):  
Viktor E Tsyganov ◽  
Anna V Tsyganova ◽  
Vera A Voroshilova ◽  
Aleksey U Borisov ◽  
Igor A Tikhonovich

Using pea single mutant lines SGEFix–-2 (sym33) and RisFixV (sym42), which are characterized by different abnormalities during symbiotic nodule development, including thickening of infection threads’ walls, a double mutant RBT4 line, carrying a pair of symbiotic genes sym33 and sym42 was constructed. The epistasis of the mutant allele sym33 over the mutant allele sym42 with respect to the histological and ultrastructural organisation of nodules was shown. Thus, it was demonstrated that Sym33 gene functions earlier in symbiotic nodule development than Sym42 gene.


2012 ◽  
Vol 81 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Wioletta Brosowska-Arendt ◽  
Stanisław Weidner

Plant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long- and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating pea (<em>Pisum sativum </em>L.) seeds. Here we show that, under osmotic stress, cytoskeleton-bound polysomes were charaterized by the highest translation activity, which may be indicative of an important role that this population of polysomes plays in the synthesis of the so-called “stress proteins”. We also find out that in response to osmotic stress, new proteins (22.01, 96.47 and 105.3 kDa), absent in the unstressed sample, associated with the total pool of polysomes, whereas the protein of 22.95 kDa, which was present in the embryonic tissue of seeds germinating under unstressed conditions, disappeared. These changes may have affected both the stability and the translational capacity of polysomes.


2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  

2015 ◽  
Vol 50 (3) ◽  
pp. 353-360
Author(s):  
М.А. ВИШНЯКОВА ◽  
◽  
Е.В. СЕМЕНОВА ◽  
И.А. КОСАРЕВА ◽  
Н.Д. КРАВЧУК ◽  
...  

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