scholarly journals Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

2012 ◽  
Vol 81 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Wioletta Brosowska-Arendt ◽  
Stanisław Weidner
Keyword(s):  
Pisum Sativum ◽  
Osmotic Stress ◽  
Stress Proteins ◽  
Short Term ◽  
Pisum Sativum L ◽  
Associated Proteins ◽  
Total Pool ◽  
The Stability ◽  
Germinating Seeds

Plant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long- and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating pea (<em>Pisum sativum </em>L.) seeds. Here we show that, under osmotic stress, cytoskeleton-bound polysomes were charaterized by the highest translation activity, which may be indicative of an important role that this population of polysomes plays in the synthesis of the so-called “stress proteins”. We also find out that in response to osmotic stress, new proteins (22.01, 96.47 and 105.3 kDa), absent in the unstressed sample, associated with the total pool of polysomes, whereas the protein of 22.95 kDa, which was present in the embryonic tissue of seeds germinating under unstressed conditions, disappeared. These changes may have affected both the stability and the translational capacity of polysomes.

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals Structural characterizations and in vitro digestibility of acid-treated wrinkled and smooth pea starch (Pisum sativum L.)

2016 ◽  
Vol 68 (7-8) ◽  
pp. 762-770 ◽  
Author(s):  
Miaomiao Shi ◽  
Kai Wang ◽  
Shujuan Yu ◽  
Robert G. Gilbert ◽  
Qunyu Gao
Keyword(s):  
Pisum Sativum ◽  
In Vitro Digestibility ◽  
Pisum Sativum L ◽  
Pea Starch ◽  
Structural Characterizations

Effect of the Drying Temperature on Color, Antioxidant Activity and In Vitro Digestibility of Green Pea ( Pisum sativum L.) Flour

2020 ◽  
Vol 72 (9-10) ◽  
pp. 1900228 ◽  
Author(s):  
Manolo Gonzalez ◽  
Jose Alvarez‐Ramirez ◽  
E. Jaime Vernon‐Carter ◽  
Isabel Reyes ◽  
Lurdes Alvarez‐Poblano
Keyword(s):  
Antioxidant Activity ◽  
Pisum Sativum ◽  
In Vitro Digestibility ◽  
Drying Temperature ◽  
Pisum Sativum L ◽  
Green Pea

scholarly journals Performance of Polyester-Based Electrospun Scaffolds under In Vitro Hydrolytic Conditions: From Short-Term to Long-Term Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 786 ◽  
Author(s):  
Oscar Gil-Castell ◽  
José David Badia ◽  
Jordi Bou ◽  
Amparo Ribes-Greus
Keyword(s):  
Molar Mass ◽  
Pure Water ◽  
Phosphate Buffer Solution ◽  
Short Term ◽  
Buffer Solution ◽  
Electrospun Scaffolds ◽  
The Stability ◽  
Short Time

The evaluation of the performance of polyesters under in vitro physiologic conditions is essential to design scaffolds with an adequate lifespan for a given application. In this line, the degradation-durability patterns of poly(lactide-co-glycolide) (PLGA), polydioxanone (PDO), polycaprolactone (PCL) and polyhydroxybutyrate (PHB) scaffolds were monitored and compared giving, as a result, a basis for the specific design of scaffolds from short-term to long-term applications. For this purpose, they were immersed in ultra-pure water and phosphate buffer solution (PBS) at 37 °C. The scaffolds for short-time applications were PLGA and PDO, in which the molar mass diminished down to 20% in a 20–30 days lifespan. While PDO developed crystallinity that prevented the geometry of the fibres, those of PLGA coalesced and collapsed. The scaffolds for long-term applications were PCL and PHB, in which the molar mass followed a progressive decrease, reaching values of 10% for PCL and almost 50% for PHB after 650 days of immersion. This resistant pattern was mainly ascribed to the stability of the crystalline domains of the fibres, in which the diameters remained almost unaffected. From the perspective of an adequate balance between the durability and degradation, this study may serve technologists as a reference point to design polyester-based scaffolds for biomedical applications.

VARIABILITY AND INTERACTION IN THE Pisum sativum L. — Rhizobium leguminosarum SYMBIOSIS

1983 ◽  
Vol 63 (3) ◽  
pp. 591-599 ◽  
Author(s):  
S. L. A. HOBBS ◽  
J. D. MAHON
Keyword(s):  
Nitrogen Fixation ◽  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Symbiotic Nitrogen Fixation ◽  
Correlation Coefficients ◽  
Bacterial Strains ◽  
Plant Genotype ◽  
Pisum Sativum L ◽  
Performance Response ◽  
The Stability

Symbiotic nitrogen fixation was examined in 36 plant genotype-bacterial strain combinations produced by growing six genotypes of Pisum sativum L. and six strains of Rhizobium leguminosarum in all combinations. Both genotypes and strains had effects not only on nitrogen fixation but also on characters associated with plant growth and photosynthesis. However, relationships between characters differed markedly depending on whether genotype or strain means were used to calculate correlation coefficients. Genotype × strain (G × S) interactions also affected the expression of several of the characters. Using nitrogen fixation as an example, statistical methods analogous to those developed for the analysis of genotype × environment interactions were used to study this interaction. From this analysis it was apparent that the G × S variability was mainly caused by differences in the magnitude of the response of plant genotypes or bacterial strains to changes in the complementary symbiont with little difference in the stability of this response. An examination of different indicators of performance, response, and stability of that response, suggests that both plant genotypes and bacterial strains could be selected for relatively uniform fixation over a range of symbiotic partners, or that specific combinations could be selected for maximum symbiotic effectiveness.Key words: Genotype × strain, N2 fixation, photosynthesis, respiration, growth

Morphogenesis of the compound leaf in three genotypes of the pea, Pisum sativum

1986 ◽  
Vol 64 (6) ◽  
pp. 1268-1276 ◽  
Author(s):  
K. S. Gould ◽  
Elizabeth G. Cutter ◽  
J. P. W. Young
Keyword(s):  
Pisum Sativum ◽  
Leaf Development ◽  
Culture System ◽  
Algebraic Model ◽  
Leaf Primordium ◽  
Axillary Shoots ◽  
Compound Leaf ◽  
Pisum Sativum L ◽  
Leaflet Anatomy

Leaf anatomy, ontogeny, and morphology were described and compared in a pea line (Pisum sativum L.) with conventional leaves and in isogenic lines carrying the mutations af (afila) or tl (tendril-less or acacia). The anatomy of stem, petiole, and rachis is not modified by these mutations. The tendrils, which in af replace leaflets, have normal tendril anatomy, and the terminal leaflets of the tl form have normal leaflet anatomy. The shoot apical dome has the same size and shape in the three genotypes, as does the leaf primordium up to the stage of initiation of the first laterals. The mature morphology of leaves varies with node of insertion. Some leaves, especially at nodes 3 and 4, have structures that are not typical of their genotype. An in vitro culture system is described for axillary shoots. Such shoots recapitulate most of the foliar features of seedling plants, but leaf morphology is on average more complex, and aberrant structures are more frequent. All these observations are discussed in relation to Young's algebraic model for compound leaf development.

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