scholarly journals Optimization of human mesenchymal stem cell isolation from synovial membrane: Implications for subsequent tissue engineering effectiveness

2016 ◽  
Vol 5 ◽  
pp. 79-85 ◽  
Author(s):  
Norihiko Sugita ◽  
Yu Moriguchi ◽  
Morito Sakaue ◽  
David A. Hart ◽  
Yukihiko Yasui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Synovial Membrane ◽  
Human Mesenchymal Stem Cell ◽  
Cell Isolation ◽  
Stem Cell Isolation

Improved Human Mesenchymal Stem Cell Isolation

2014 ◽  
Vol 23 (4-5) ◽  
pp. 399-406 ◽  
Author(s):  
Tzu-Min Chan ◽  
Horng-Jyh Harn ◽  
Hui-Ping Lin ◽  
Pei-Wen Chou ◽  
Julia Yi-Ru Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Cell Isolation ◽  
Stem Cell Isolation

scholarly journals 27: Progress in Regenerative Medicine Using Cord Blood: Mesenchymal Stem Cell Isolation and Induction to Bone and Cartilage

2007 ◽  
Vol 13 (11) ◽  
pp. 1402 ◽  
Author(s):  
Tsuneo A. Takahashi ◽  
Xiaohong Zhang ◽  
Koichi Igura ◽  
Toshihide Nishimura ◽  
Ayako Mitsuru ◽  
...  
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Mesenchymal Stem Cell ◽  
Cord Blood ◽  
Cell Isolation ◽  
Stem Cell Isolation ◽  
And Cartilage

Mesenchymal stem cell isolation and characterization from human spinal ligaments

2012 ◽  
Vol 417 (4) ◽  
pp. 1193-1199 ◽  
Author(s):  
Toru Asari ◽  
Ken-Ichi Furukawa ◽  
Sunao Tanaka ◽  
Hitoshi Kudo ◽  
Hiroki Mizukami ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Isolation ◽  
Isolation And Characterization ◽  
Spinal Ligaments ◽  
Stem Cell Isolation

scholarly journals 3D Printed Wavy Scaffolds Enhance Mesenchymal Stem Cell Osteogenesis

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 31 ◽  
Author(s):  
Shen Ji ◽  
Murat Guvendiren
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Mesenchymal Stem Cell ◽  
Calcium Deposition ◽  
Porous Scaffolds ◽  
Osteogenic Lineage ◽  
3D Printed

There is a growing interest in developing 3D porous scaffolds with tunable architectures for bone tissue engineering. Surface topography has been shown to control stem cell behavior including differentiation. In this study, we printed 3D porous scaffolds with wavy or linear patterns to investigate the effect of wavy scaffold architecture on human mesenchymal stem cell (hMSC) osteogenesis. Five distinct wavy scaffolds were designed using sinusoidal waveforms with varying wavelengths and amplitudes, and orthogonal scaffolds were designed using linear patterns. We found that hMSCs attached to wavy patterns, spread by taking the shape of the curvatures presented by the wavy patterns, exhibited an elongated shape and mature focal adhesion points, and differentiated into the osteogenic lineage. When compared to orthogonal scaffolds, hMSCs on wavy scaffolds showed significantly enhanced osteogenesis, indicated by higher calcium deposition, alkaline phosphatase activity, and osteocalcin staining. This study aids in the development of 3D scaffolds with novel architectures to direct stem osteogenesis for bone tissue engineering.

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