Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force

2007 ◽  
Vol 82B (2) ◽  
pp. 471-480 ◽  
Author(s):  
Kazunori Shimizu ◽  
Akira Ito ◽  
Tatsuro Yoshida ◽  
Yoichi Yamada ◽  
Minoru Ueda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Magnetite Nanoparticles ◽  
Magnetic Force ◽  
Human Mesenchymal Stem Cell ◽  
Cell Sheets

scholarly journals 3D Printed Wavy Scaffolds Enhance Mesenchymal Stem Cell Osteogenesis

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 31 ◽  
Author(s):  
Shen Ji ◽  
Murat Guvendiren
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Mesenchymal Stem Cell ◽  
Calcium Deposition ◽  
Porous Scaffolds ◽  
Osteogenic Lineage ◽  
3D Printed

There is a growing interest in developing 3D porous scaffolds with tunable architectures for bone tissue engineering. Surface topography has been shown to control stem cell behavior including differentiation. In this study, we printed 3D porous scaffolds with wavy or linear patterns to investigate the effect of wavy scaffold architecture on human mesenchymal stem cell (hMSC) osteogenesis. Five distinct wavy scaffolds were designed using sinusoidal waveforms with varying wavelengths and amplitudes, and orthogonal scaffolds were designed using linear patterns. We found that hMSCs attached to wavy patterns, spread by taking the shape of the curvatures presented by the wavy patterns, exhibited an elongated shape and mature focal adhesion points, and differentiated into the osteogenic lineage. When compared to orthogonal scaffolds, hMSCs on wavy scaffolds showed significantly enhanced osteogenesis, indicated by higher calcium deposition, alkaline phosphatase activity, and osteocalcin staining. This study aids in the development of 3D scaffolds with novel architectures to direct stem osteogenesis for bone tissue engineering.

scholarly journals Scaffold-free human mesenchymal stem cell construct geometry regulates long bone regeneration

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Samuel Herberg ◽  
Daniel Varghai ◽  
Daniel S. Alt ◽  
Phuong N. Dang ◽  
Honghyun Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Mesenchymal Stem Cell ◽  
Functional Restoration ◽  
Defect Healing ◽  
Tgf Β1

AbstractBiomimetic bone tissue engineering strategies partially recapitulate development. We recently showed functional restoration of femoral defects using scaffold-free human mesenchymal stem cell (hMSC) condensates featuring localized morphogen presentation with delayed in vivo mechanical loading. Possible effects of construct geometry on healing outcome remain unclear. Here, we hypothesized that localized presentation of transforming growth factor (TGF)-β1 and bone morphogenetic protein (BMP)-2 to engineered hMSC tubes mimicking femoral diaphyses induces endochondral ossification, and that TGF-β1 + BMP-2-presenting hMSC tubes enhance defect healing with delayed in vivo loading vs. loosely packed hMSC sheets. Localized morphogen presentation stimulated chondrogenic priming/endochondral differentiation in vitro. Subcutaneously, hMSC tubes formed cartilage templates that underwent bony remodeling. Orthotopically, hMSC tubes stimulated more robust endochondral defect healing vs. hMSC sheets. Tissue resembling normal growth plate was observed with negligible ectopic bone. This study demonstrates interactions between hMSC condensation geometry, morphogen bioavailability, and mechanical cues to recapitulate development for biomimetic bone tissue engineering.

scholarly journals Mesenchymal Stem Cell–Encapsulated Collagen Microspheres for Bone Tissue Engineering

2010 ◽  
Vol 16 (2) ◽  
pp. 225-235 ◽  
Author(s):  
Barbara Pui Chan ◽  
Ting Yan Hui ◽  
Mei Yi Wong ◽  
Kevin Hak Kong Yip ◽  
Godfrey Chi Fung Chan
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering

2019 ◽  
Vol 11 (9) ◽  
pp. 8749-8762 ◽  
Author(s):  
Chen Zhao ◽  
Nader Taheri Qazvini ◽  
Monirosadat Sadati ◽  
Zongyue Zeng ◽  
Shifeng Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hydrogel Scaffold ◽  
Hybrid Hydrogel ◽  
Self Assembled
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