In vitro aerobic and anaerobic muscle capacities in the European eel, Anguilla anguilla: Effects of a swimming session

2011 ◽  
Vol 176 (3) ◽  
pp. 118-122 ◽  
Author(s):  
Philippe Sébert ◽  
Hélène Mortelette ◽  
Jonathan Nicolas ◽  
Aline Amérand ◽  
Marc Belhomme ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Aerobic And Anaerobic

Effects of salinity and sea salt type on egg activation, fertilization, buoyancy and early embryology of European eel, Anguilla anguilla

Zygote ◽  
2015 ◽  
Vol 24 (1) ◽  
pp. 121-138 ◽  
Author(s):  
Sune Riis Sørensen ◽  
Ian Anthony Ernest Butts ◽  
Peter Munk ◽  
Jonna Tomkiewicz
Keyword(s):  
Ionic Composition ◽  
Anguilla Anguilla ◽  
Fertilization Success ◽  
Sea Salt ◽  
European Eel ◽  
Natural Seawater ◽  
Neutral Buoyancy ◽  
Cell Cleavage ◽  
Practical Salinity Unit

SummaryImproper activation and swelling of in vitro produced eggs of European eel, Anguilla anguilla, has been shown to negatively affect embryonic development and hatching. We investigated this phenomenon by examining the effects of salinity and sea salt type on egg dimensions, cell cleavage patterns and egg buoyancy. Egg diameter after activation, using natural seawater adjusted to different salinities, varied among female eels, but no consistent pattern emerged. Activation salinities between 30–40 practical salinity unit (psu) produced higher quality eggs and generally larger egg diameters. Chorion diameters reached maximal values of 1642 ± 8 μm at 35 psu. A positive relationship was found between egg neutral buoyancy and activation salinity. Nine salt types were investigated as activation and incubation media. Five of these types induced a substantial perivitelline space (PVS), leading to large egg sizes, while the remaining four salt types resulted in smaller eggs. All salt types except NaCl treatments led to high fertilization rates and had no effect on fertilization success as well as egg neutral buoyancies at 7 h post-fertilization. The study points to the importance of considering ionic composition of the media when rearing fish eggs and further studies are encouraged.

FAILURE OF CORPUSCLES OF STANNIUS OF THE EUROPEAN EEL (ANGUILLA ANGUILLA L.) TO PRODUCE CORTICOSTEROIDS IN VITRO

1965 ◽  
Vol 33 (2) ◽  
pp. 319-320 ◽  
Author(s):  
I. CHESTER JONES ◽  
D. K. O. CHAN ◽  
I. W. HENDERSON ◽  
W. MOSLEY ◽  
T. SANDOR ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Corpuscles Of Stannius

Steroidogenesis in Vitro by Homogenates of Adrenocortical Tissue of the European Eel (Anguilla anguilla L.)1

Endocrinology ◽  
1967 ◽  
Vol 81 (4) ◽  
pp. 904-912 ◽  
Author(s):  
T. SANDOR ◽  
A. LANTHIER ◽  
I. W. HENDERSON ◽  
I. CHESTER JONES
Keyword(s):  
Anguilla Anguilla ◽  
European Eel

Penetration of horseradish peroxidase into the extravascular channel system of in vitro European eel (Anguilla anguilla) pars distalis, with comments on the role of the nongranulated cells

1982 ◽  
Vol 60 (5) ◽  
pp. 838-847 ◽  
Author(s):  
John F. Leatherland ◽  
Bridget I. Baker
Keyword(s):  
Horseradish Peroxidase ◽  
Cell Function ◽  
Cell Activity ◽  
Anguilla Anguilla ◽  
Metabolic Inhibitors ◽  
European Eel ◽  
Pars Distalis ◽  
Large Molecules

Horseradish peroxidase (HPO) was used as an extracellular space marker to investigate the characteristics of the extravascular network in in vitro cultured hemipituitaries of Anguilla anguilla. HPO penetrated large extravascular channels in both the rostral and proximal pars distalis. In the rostral pars distalis the channels were associated with the base of the columnar prolactin cells and in the basal lamina between the corticotropes and anterior neurohypophysis in the dorsal region. In the proximal pars distalis the channels separated the cords of granulated cells. HPO also penetrated into fine extracellular spaces, which appeared to be continuous with the extravascular channels, in both rostral and proximal pars distalis. HPO penetrated only those extracellular spaces associated with the nongranulated (NG) cells, suggesting a related function between the movement of large molecules in the extravascular network and NG cell function. However, in in vitro situations which alter pars distalis cell activity (altered osmotic pressure of the incubation medium or incubation in the presence of metabolic inhibitors such as dinitrophenol or KCN) penetration of HPO did not appear to be affected, suggesting that the HPO penetration was passive, at least in the in vitro conditions used here.

The biosynthesis of 18-hydroxycorticosterone from exogenous corticosterone by teleost fish adrenocortical tissue in vitro

1970 ◽  
Vol 48 (5) ◽  
pp. 553-558 ◽  
Author(s):  
T. Sandor ◽  
S. W. C. Chan ◽  
J. G. Phillips ◽  
D. Ensor ◽  
I. W. Henderson ◽  
...  
Keyword(s):  
Gadus Morhua ◽  
Sea Water ◽  
Kidney Tissue ◽  
Head Kidney ◽  
Anguilla Anguilla ◽  
Melanogrammus Aeglefinus ◽  
European Eel ◽  
Kidney Mitochondria ◽  
Excess Substrate

Incubation of homogenized head kidney tissue of the cod (Gadus morhua) and the haddock (Melanogrammus aeglefinus) with tritiated corticosterone resulted in the formation of small amounts of 18-hydroxycorticosterone (cod 0.04%, haddock 0.012% transformation per 6 g head kidney tissue per 4 h), in the presence of excess NADPH. Incubation of European eel (Anguilla anguilla) posterior vein and head kidney "mitochondria" with corticosterone-4-14C, in the presence of excess substrate and NADPH, also gave rise to 18-hydroxycorticosterone (fresh-water-adapted animals, 0.13% per mitochondria equivalent to 756 mg tissue per 2 h; animals adapted to sea water for 48 h, 0.09% per mitochondria equivalent to 456 mg tissue per 2 h). The biosynthetic, isotopically labelled 18-hydroxycorticosterone was identified by serial oxidation. Head kidneys of both the cod and haddock transformed corticosterone to cortisol. However, the unequivocal presence of aldosterone could not be shown in any of these experiments.

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