Enzyme recycling could break down plastics by 70%

2020 ◽  
Vol 64 (5) ◽  
pp. 237
Keyword(s):  
Enzyme Recycling

KINETIC AND ENZYME RECYCLING STUDIES OF IMMOBILIZED b-GLUCOSIDASE FOR LIGNOCELLULOSIC BIOMASS HYDROLYSIS

2018 ◽  
Vol 17 (6) ◽  
pp. 1385-1398 ◽  
Author(s):  
Deepak K. Tuli ◽  
Ruchi Agrawal ◽  
Alok Satlewal ◽  
Anshu S. Mathur ◽  
Ravi P. Gupta ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Biomass Hydrolysis ◽  
Enzyme Recycling

scholarly journals Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1558 ◽  
Author(s):  
Zhang ◽  
Dong ◽  
Zhou ◽  
Hu ◽  
Li ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Immobilized Enzyme ◽  
Coiled Coil ◽  
Retention Rates ◽  
Generate Functional ◽  
Retention Efficiency ◽  
Enzyme Recycling ◽  
Lysine Residues ◽  
Target Molecules ◽  
Good Retention

Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling. Here, we report the noncovalent immobilization of a genetically modified β-glucosidase onto the outer surface of synthetic magnetoferritin through the electrostatic interaction of a heterodimeric coiled-coil protein formed by coils containing lysine residues (K-coils) and coils containing glutamic acid (E-coils). The immobilized enzyme was characterized, and its enzymatic properties were evaluated. Furthermore, reusability of immobilized enzyme was demonstrated in aqueous solution under an applied magnetic field. The results showed that magnetoferritin was successfully prepared and it was an excellent support for enzyme immobilization. After three times usages, the retention rates were 93.75%, 82.5%, and 56.25%, respectively, demonstrating that immobilized enzyme possessed good retention efficiency and could be used as potential carrier for other biomolecules. The strategy of enzyme immobilization developed in this work can be applied, in general, to many other target molecules.

scholarly journals GORAB scaffolds COPI at the trans-Golgi for efficient enzyme recycling and correct protein glycosylation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Tomasz M. Witkos ◽  
Wing Lee Chan ◽  
Merja Joensuu ◽  
Manuel Rhiel ◽  
Ed Pallister ◽  
...  
Keyword(s):  
Protein Glycosylation ◽  
Enzyme Recycling
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