A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse

2012 ◽  
Vol 5 (5) ◽  
pp. 7168 ◽  
Author(s):  
Mingjie Jin ◽  
Christa Gunawan ◽  
Nirmal Uppugundla ◽  
Venkatesh Balan ◽  
Bruce E. Dale
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Biological Process ◽  
Ethanol Productivity ◽  
Yeast Cells ◽  
Enzyme Recycling ◽  
High Ethanol

Cellulosic ethanol production by combination of cellulase-displaying yeast cells

2012 ◽  
Vol 51 (6-7) ◽  
pp. 366-372 ◽  
Author(s):  
Seung-Ho Baek ◽  
Sujin Kim ◽  
Kyusung Lee ◽  
Jung-Kul Lee ◽  
Ji-Sook Hahn
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Yeast Cells

scholarly journals Optimization of Yeast, Sugar and Nutrient Concentrations for High Ethanol Production Rate Using Industrial Sugar Beet Molasses and Response Surface Methodology

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 86
Author(s):  
Jean-Baptiste Beigbeder ◽  
Julia Maria de Medeiros Dantas ◽  
Jean-Michel Lavoie
Keyword(s):  
Response Surface Methodology ◽  
Sugar Beet ◽  
Ethanol Production ◽  
Response Surface ◽  
Ethanol Productivity ◽  
Sugar Concentration ◽  
Yeast Fermentation ◽  
Beet Molasses ◽  
Sugar Beet Molasses ◽  
High Ethanol

Among the various agro-industrial by-products, sugar beet molasses produced by sugar refineries appear as a potential feedstock for ethanol production through yeast fermentation. A response surface methodology (RSM) was developed to better understand the effect of three process parameters (concentration of nutrient, yeast and initial sugar) on the ethanol productivity using diluted sugar beet molasses and Saccharomyces cerevisiae yeast. The first set of experiments performed at lab-scale indicated that the addition of 4 g/L of nutrient combined with a minimum of 0.2 g/L of yeast as well as a sugar concentration lower than 225 g/L was required to achieve high ethanol productivities (<15 g/L/d). The optimization allowed to considerably reduce the amount of yeast initially introduced in the fermentation substrate while still maximizing both ethanol productivity and yield process responses. Finally, scale-up assays were carried out in 7.5 and 100 L bioreactors using the optimal conditions: 150 g/L of initial sugar concentration, 0.27 g/L of yeast and 4 g/L of nutrient. Within 48 h of incubation, up to 65 g/L of ethanol were produced for both scales, corresponding to an average ethanol yield and sugar utilization rate of 82% and 85%, respectively. The results obtained in this study highlight the use of sugar beet molasses as a low-cost food residue for the sustainable production of bioethanol.

scholarly journals Development of High-Productivity Continuous Ethanol Production using PVA-Immobilized Zymomonas mobilis in an Immobilized-Cells Fermenter

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Nurhayati Nurhayati ◽  
Chieh-Lun Cheng ◽  
Jo-Shu Chang
Keyword(s):  
Ethanol Production ◽  
Dilution Rate ◽  
Ethanol Fermentation ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Productivity ◽  
Glucose Loading ◽  
Continuous Ethanol Fermentation ◽  
Residual Glucose ◽  
High Ethanol

Ethanol as one of renewable energy was being considered an excellent alternative clean-burning fuel to replace gasoline. Continuous ethanol fermentation systems had offered important economic advantages compared to traditional systems. Fermentation rates were significantly improved, especially when continuous fermentation was integrated with cell immobilization techniques to enrich the cells concentration in fermentor. Growing cells of Zymomonas mobilis immobilized in polyvinyl alcohol (PVA) gel beads were employed in an immobilized-cells fermentor for continuous ethanol fermentation from glucose. The glucose loading, dilution rate, and cells loading were varied in order to determine which best condition employed in obtaining both high ethanol production and low residual glucose with high dilution rate. In this study, 20 g/L, 100 g/L, 125 g/L and 150 g/L of glucose concentration and 20% (w/v), 40% (w/v) and 50% (w/v) of cells loading were employed with range of dilution rate at 0.25 to 1 h-1. The most stable production was obtained for 25 days by employing 100 g/L of glucose loading. Meanwhile, the results also exhibited that 125 g/L of glucose loading as well as 40% (w/v) of cells loading yielded high ethanol concentration, high ethanol productivity, and acceptable residual glucose at 62.97 g/L, 15.74 g/L/h and 0.16 g/L, respectively. Furthermore, the dilution rate of 4 hour with 100 g/L and 40% (w/v) of glucose and cells loading was considered as the optimum condition with ethanol production, ethanol productivity and residual glucose obtained were 49.89 g/L, 12.47 g/L/h, and 2.04 g/L, respectively. This recent study investigated ethanol inhibition as well. The present research had proved that high sugar concentration was successfully converted to ethanol. These achieved results were promising for further study.

scholarly journals Evaluating crude whey for bioethanol production using non-Saccharomyces yeast, Kluyveromyces marxianus

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Asmamaw Tesfaw ◽  
Ebru Toksoy Oner ◽  
Fassil Assefa
Keyword(s):  
Ethanol Production ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Food Production ◽  
Ethanol Productivity ◽  
Bioethanol Production ◽  
Ph Adjustment ◽  
Saccharomyces Yeast ◽  
Food Substrate ◽  
High Ethanol

AbstractEthanol production from non-food substrate is strongly recommended to avoid competition with food production. Whey, which is rich in nutrients, is one of the non-food substrate for ethanol production by Kluyveromyces spp. The purpose of this study was to optimize ethanol from different crude (non-deproteinized, non-pH adjusted, and non-diluted) whey using K. marxianus ETP87 which was isolated from traditional yoghurt. The sterilized and non-sterilized whey were employed for K. marxianus ETP87 substrate to evaluate the yeast competition potential with lactic acid and other microflora in whey. The effect of pH and temperature on ethanol productivity from whey was also investigated. Peptone, yeast extract, ammonium sulfate ((NH4)2SO4), and urea were supplemented to whey in order to investigate the requirement of additional nutrient for ethanol optimization. The ethanol obtained from non-sterilized whey was slightly and statistically lower than sterilized whey. The whey storage at 4 °C didn’t guarantee the constant lactose presence at longer preservation time. Significantly high amount of ethanol was attained from whey without pH adjustment (3.9) even if it was lower than pH controlled (5.0) whey. The thermophilic yeast, K. marxianus ETP87, yielded high ethanol between 30 and 35 °C, and the yeast was able to produce high ethanol until 45 °C, and significantly lower ethanol was recorded at 50 °C. The ammonium sulfate and peptone enhanced ethanol productivity, whereas yeast extract and urea depressed the yeast ethanol fermentation capability. The K. marxianus ETP87, the yeast isolated from traditional yoghurt, is capable of producing ethanol from non-sterilized and non-deproteinized substrates.

scholarly journals Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

2007 ◽  
Vol 73 (7) ◽  
pp. 2349-2353 ◽  
Author(s):  
Shinsuke Sakai ◽  
Yoshiki Tsuchida ◽  
Shohei Okino ◽  
Osamu Ichihashi ◽  
Hideo Kawaguchi ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Acids ◽  
Ethanol Production ◽  
Corynebacterium Glutamicum ◽  
Cellulosic Ethanol ◽  
Ethanol Productivity ◽  
Fermentation Inhibitors

ABSTRACT In cellulosic ethanol production, pretreatment of a biomass to facilitate enzymatic hydrolysis inevitably yields fermentation inhibitors such as organic acids, furans, and phenols. With representative inhibitors included in the medium at various concentrations, individually or in various combinations, ethanol production by Corynebacterium glutamicum R under growth-arrested conditions was investigated. In the presence of various inhibitors, the 62 to 100% ethanol productivity retained by the C. glutamicum R-dependent method far exceeded that retained by previously reported methods.

Vitalized yeast with high ethanol productivity

RSC Advances ◽  
2014 ◽  
Vol 4 (94) ◽  
pp. 52299-52306 ◽  
Author(s):  
Xiumei Liu ◽  
Wenjuan Xu ◽  
Chao Zhang ◽  
Peifang Yan ◽  
Songyan Jia ◽  
...  
Keyword(s):  
Water Soluble ◽  
Ethanol Productivity ◽  
Yeast Cells ◽  
High Ethanol

Yeast cells exo-protected by water soluble PEGs are tolerant to higher ethanol concentrations, and offer high ethanol productivity.

Production and Application of Lignin-Based Chemicals and Materials in the Cellulosic Ethanol Production: An Overview on Lignin Closed-Loop Biorefinery Approaches

2021 ◽  
Author(s):  
Carlos Eduardo de Araújo Padilha ◽  
Cleitiane da Costa Nogueira ◽  
Bárbara Ribeiro Alves Alencar ◽  
Íthalo Barbosa Silva de Abreu ◽  
Emmanuel Damilano Dutra ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Closed Loop

scholarly journals Utilization of Agro-Industrial Residues and Municipal Waste of Plant Origin for Cellulosic Ethanol Production

2011 ◽  
Vol 02 (10) ◽  
pp. 1303-1309 ◽  
Author(s):  
Fabiano A. Gonçalves ◽  
Eliana J. Sanjinez-Argandoña ◽  
Gustavo G. Fonseca
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Municipal Waste ◽  
Plant Origin ◽  
Industrial Residues
Sign in / Sign up

Export Citation Format

Share Document