Direct in vitro effect of LH and steroids on leptin secretion by porcine luteal cells during the late-luteal phase of the estrous cycle

2013 ◽  
Vol 13 ◽  
pp. 43
Author(s):  
Gabriela Siawrys
Keyword(s):  
Estrous Cycle ◽  
Luteal Phase ◽  
Luteal Cells ◽  
Late Luteal Phase ◽  
Vitro Effect ◽  
Porcine Luteal Cells

scholarly journals Is interleukin-1α a luteotrophic or luteolytic agent in cattle?

Reproduction ◽  
2010 ◽  
Vol 139 (3) ◽  
pp. 665-672 ◽  
Author(s):  
Magdalena Majewska ◽  
Izabela Woclawek-Potocka ◽  
Mamadou M Bah ◽  
Joanna Hapunik ◽  
Katarzyna K Piotrowska ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Early Pregnancy ◽  
Luteal Phase ◽  
Late Luteal Phase ◽  
Interleukin 1Α ◽  
And Function ◽  
Different Doses

Cytokines are thought to regulate prostaglandin (PG) secretion in the bovine endometrium. However, there is no consensus about the role of interleukin-1α (IL1A) on PG secretion. The objective of this study was to examine the influence of IL1A on basal and interferon-τ (IFNT)-regulated PGin vitrosecretion, as well its effects on PG secretion, progesterone (P4) output, and corpus luteum (CL)in vivolifespan. Explants of bovine endometrium (days 16–17 of the estrous cycle or early pregnancy) were stimulated with IL1A (10 ng/ml), IFNT (30 ng/ml), or IL1A combined with IFN. IL1A alone strongly stimulated luteotrophic PGE2secretion by endometrial tissues of both pregnant and nonpregnant cows. IL1A also stimulated luteolytic PGF2αoutput in the late luteal phase. IFNT augmented the stimulatory effect of IL1A on PGE2secretion. In anin vivoexperiment, saline or IL1A at different doses (0.001–10 μg/per animal) was applied to the uterine lumen on day 16 of the cycle. Only the highest dose of IL1A caused a temporal increase in PGF2αsecretion, while it had no effect on P4secretion or CL lifespan. Application of 0.1 and 1 μg IL1A stimulated P4and PGE2output and prolonged the CL lifespan. Although IL1A may stimulatein vitroluteolytic PGF2αsecretion during the estrous cycle, it only acts as a luteotrophic factorin vivo. IL1A increased luteotrophic PGE2and P4output, inhibiting spontaneous luteolysis. These luteotrophic effects may result in appropriate luteal development and function in cows during the estrous cycle and early pregnancy.

scholarly journals The In Vitro Effect of Steroid Hormones, Arachidonic Acid, and Kinases Inhibitors on Aquaporin 1, 2, 5, and 7 Gene Expression in the Porcine Uterine Luminal Epithelial Cells during the Estrous Cycle

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 832
Author(s):  
Damian Tanski ◽  
Agnieszka Skowronska ◽  
Malgorzata Tanska ◽  
Ewa Lepiarczyk ◽  
Mariusz T. Skowronski
Keyword(s):  
Arachidonic Acid ◽  
Epithelial Cells ◽  
Steroid Hormones ◽  
Estrous Cycle ◽  
Kinase Inhibitor ◽  
Mapk Signaling ◽  
Mapk Kinase ◽  
Vitro Effect ◽  
Luminal Epithelial Cells

Aquaporins (AQPs) are integral membrane proteins, which play an important role in water homeostasis in the uterus. According to the literature, the expression of aquaporins in reproductive structures depends on the local hormonal milieu. The current study investigated the effect of selected PKA kinase inhibitor H89 and MAPK kinase inhibitor PD98059, on the expression of AQP1, 2, 5, and 7, and steroid hormones (E2), progesterone (P4), and arachidonic acid (AA) in the porcine endometrium on days 18–20 and 2–4 of the estrous cycle (the follicular phase where estrogen and follicle-stimulating hormone (FSH) are secreted increasingly in preparation for estrus and the luteal phase where the ovarian follicles begin the process of luteinization with the formation of the corpus luteum and progesterone secretion, respectively). The luminal epithelial cells were incubated in vitro in the presence of the aforementioned factors. The expression of mRNA was determined by the quantitative real-time PCR technique. In general, in Experiment 1, steroid hormones significantly increased expression of AQP1, 2, and 5 while arachidonic acid increased expression of AQP2 and AQP7. On the other hand, MAPK kinase inhibitor significantly decreased the expression of AQP1 and 5. In Experiment 2, E2, P4, or AA combined with kinase inhibitors differentially affected on AQPs expression. E2 in combination with PKA inhibitor significantly decreased expression of AQP1 but E2 or P4 combined with this inhibitor increased the expression of AQP5 and 7. On the contrary, E2 with PD98059 significantly increased AQP5 and AQP7 expression. Progesterone in combination with MAPK kinase inhibitor significantly downregulated the expression of AQP5 and upregulated AQP7. Arachidonic acid mixed with H89 or PD98059 caused a decrease in the expression of AQP5 and an increase of AQP7. The obtained results indicate that estradiol, progesterone, and arachidonic acid through PKA and MAPK signaling pathways regulate the expression of AQP1 and AQP5 in the porcine luminal epithelial cells in the periovulatory period.

scholarly journals Luteotrophic effects of relaxin, chorionic gonadotrophin and FSH in common marmoset monkeys (Callithrix jacchus)

Reproduction ◽  
2010 ◽  
Vol 139 (5) ◽  
pp. 923-930 ◽  
Author(s):  
Nicola Beindorff ◽  
Almuth Einspanier
Keyword(s):  
Early Pregnancy ◽  
Luteal Phase ◽  
Common Marmoset ◽  
Chorionic Gonadotrophin ◽  
Traditional Role ◽  
Local Effects ◽  
Ringer’S Solution ◽  
Late Luteal Phase ◽  
Marmoset Monkeys

In early pregnant primates, relaxin (RLX) is highly upregulated within the corpus luteum (CL), suggesting that RLX may have an important role in the implantation of the blastocyst. Therefore, the aim of the present study was to investigate the local effects of RLX and gonadotrophins on the maintenance of the CL using anin vitromicrodialysis system. CLs of common marmoset monkeys were collected by luteectomy during different stages of the luteal phase and early pregnancy. Each CL was perfused with either Ringer's solution alone or Ringer's solution supplemented with either porcine RLX (250, 500 and 1000 ng/ml) or gonadotrophins (50 IU/ml). Application of RLX provoked a significant luteal response of progesterone (P4) and oestradiol (E2) secretions during the mid-luteal phase (500 ng/ml: P454±42%, E224±11%; 1000 ng/ml: E216±13%), and especially during the late luteal phase (250 ng/ml: P453±10%; 500 ng/ml: P444±15%; 1000 ng/ml: P462±15%, E218±7%). The effects of RLX on steroid secretion were irrespective of the RLX dosages. While treatment with human chorionic gonadotrophin did not affect luteal steroid or RLX secretion, the application of FSH resulted in a significant increase in the secretion of both P4(20±8%) and E2(37±28%), and a prominent rise in RLX during early pregnancy. In conclusion, our results demonstrate that RLX and FSH have a luteotrophic function in the marmoset monkeys; moreover, FSH has a function beyond its traditional role just as a follicle-stimulating hormone.

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

scholarly journals Evidence for a PGF2α auto-amplification system in the endometrium in mares

Reproduction ◽  
2016 ◽  
Vol 151 (5) ◽  
pp. 517-526 ◽  
Author(s):  
Keisuke Kozai ◽  
Shota Tokuyama ◽  
Anna Z Szóstek ◽  
Yuko Toishi ◽  
Nobuo Tsunoda ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Stromal Cells ◽  
Luteal Phase ◽  
Endometrial Cell ◽  
Late Luteal Phase ◽  
Prostaglandin F ◽  
Amplification System ◽  
Prostaglandin Endoperoxide Synthase

AbstractIn mares, prostaglandin F2α(PGF2α) secreted from the endometrium is a major luteolysin. Some domestic animals have an auto-amplification system in which PGF2αcan stimulate its own production. Here, we investigated whether this is also the case in mares. In anin vivostudy, mares at the mid-luteal phase (days 6–8 of estrous cycle) were injected i.m. with cloprostenol (250 µg) and blood samples were collected at fixed intervals until 72 h after treatment. Progesterone (P4) concentrations started decreasing 45 min after the injection and continued to decrease up to 24 h (P < 0.05). In turn, 13,14-dihydro-15-keto-PGF2α(PGFM) metabolite started to increase 4h after an injection and continued to increase up to 72 h (P < 0.05). PGF receptor (PTGFR) mRNA expression in the endometrium was significantly higher in the late luteal phase than in the early and regressed luteal phases (P < 0.05).In vitro, PGF2αsignificantly stimulated (P < 0.05) PGF2αproduction by endometrial tissues and endometrial epithelial and stromal cells and significantly increased (P < 0.05) the mRNA expression of prostaglandin-endoperoxide synthase-2 (PTGS2), an enzyme involved in PGF2αsynthesis in endometrial cell. These findings strongly suggest the existence of an endometrial PGF2αauto-amplification system in mares.

Effects of Oxytocin on in Vitro Steroid Release of Midstage Small and Large Porcine Luteal Cells*

Endocrinology ◽  
1990 ◽  
Vol 126 (5) ◽  
pp. 2343-2349 ◽  
Author(s):  
LUTZ PITZEL ◽  
HUBERTUS JARRY ◽  
WOLFGANG WUTTKE
Keyword(s):  
Luteal Cells ◽  
Porcine Luteal Cells

Cellular basis of luteal steroidogenesis in the human ovary

1989 ◽  
Vol 122 (1) ◽  
pp. 303-NP ◽  
Author(s):  
B. Fisch ◽  
R. A. Margara ◽  
R. M. L. Winston ◽  
S. G. Hillier
Keyword(s):  
Luteal Phase ◽  
Culture System ◽  
Direct Action ◽  
Cellular Level ◽  
Luteal Cell ◽  
Aromatase Activity ◽  
Vitro Fertilization ◽  
Luteal Cells ◽  
Expected Time

ABSTRACT A primary monolayer cell culture system was developed to investigate human corpus luteum (CL) function in vitro. Steroidogenic cells were isolated by collagenase dispersal and Percoll density-gradient fractionation from CLs enucleated at progressive stages of the luteal phase (tubal surgery patients). 'Pure' granulosa-lutein cells were aspirated from ovulatory follicles at mid-cycle (in-vitro fertilization patients). The steroidogenic capacity (progesterone/20α-dihydroprogesterone biosynthesis and aromatase activity) of isolated luteal cells was assessed in relation to CL development. Basal luteal cell steroidogenesis was maximal at around the expected time of ovulation and declined with CL age during the luteal phase. Conversely, human chorionic gonadotrophin (hCG)-responsive steroidogenesis was initially undetectable but developed as the luteal phase progressed. These results show that luteal cell steroidogenesis becomes increasingly dependent upon gonadotrophic support with CL age. This is evidence that functional luteolysis in human ovaries (1) is pre-programmed to occur at the cellular level, (2) is initiated automatically at the time of ovulation and (3) is reversed at the time of CL 'rescue' in early pregnancy by the direct action of trophoblastic hCG on steroidogenic luteal cells. The culture system described should be of value in further defining the control of human CL form and function at the cellular level. Journal of Endocrinology (1989) 122, 303–311

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