Evaluation of the physiological value of porcine luteal cells isolated in various stages of the luteal phase: Tissue culture approach

1992 ◽  
Vol 8 (3) ◽  
pp. 215-217 ◽  
Author(s):  
E. L. Gregoraszczuk ◽  
A. Wojtusiak
Keyword(s):  
Tissue Culture ◽  
Luteal Phase ◽  
Luteal Cells ◽  
Porcine Luteal Cells ◽  
Physiological Value

Growth hormone and insulin-like growth factor-I action on progesterone secretion by porcine corpora lutea isolated at various periods of the luteal phase

2003 ◽  
Vol 51 (2) ◽  
pp. 197-208 ◽  
Author(s):  
Anna Ptak ◽  
Ewa L. Gregoraszczuk ◽  
J. Rząsa
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Luteal Phase ◽  
Actinomycin D ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Factor I ◽  
Progesterone Secretion ◽  
Igf I ◽  
Porcine Luteal Cells

This study was conducted to investigate the interactions between growth hormone (GH) and insulin-like growth factor-I (IGF-I) on progesterone (P4) secretion by porcine luteal cells cultured in vitro. Cells isolated from corpora lutea (CL) collected at three different periods of the luteal phase (CL1 - early luteal phase; CL2 - middle luteal phase and CL3 - late luteal phase) were incubated with different doses of GH (10, 100 or 200 ng/ml). After 48 h cultures were terminated and the media were frozen until further P4 concentration analysis. GH (100 ng/ml) increased P4 secretion by CL1 and CL2 and had no effect on CL3. In separate studies these cells were treated for 48 h with IGF-I alone or with GH combined with IGF-I. IGF-I alone increased basal P4 secretion only by cells collected from CL1 while concurrent treatment with GH had no effect on P4 secretion by any type of CL. To investigate the possible mechanism of GH and IGF-I mediated induction of P4 secretion, an inhibitory study was conducted. In this experiment, luteal cells collected from CL1 were cultured in the absence or presence of cycloheximide (an inhibitor of protein synthesis) or actinomycin D (an inhibitor of DNA transcription). Cycloheximide or actinomycin D completely blocked the stimulatory effect of both GH and IGF-I on P4 production but did not reduce basal progesterone secretion suggesting involvement of gene transcription and translation in the GH and IGF-I action on luteal cells. Additionally, the activity of 3ß-hydroxysteroid dehydrogenase (3ß-HSD) under the influence of GH added alone or together with IGF was measured by the conversion of pregnenolone to progesterone. Stimulation of P4 secretion in P5-treated cells in GH-stimulated cultures was not observed, however, high stimulatory effect was noted in IGF-I treated cultures. In conclusion, the present studies indicate that there is direct and cycle stage dependent influence of GH and IGF-I on steroidogenesis in porcine luteal cells. It is suggested that both IGF and GH may exert some regulatory action during CL development in the pig.

Mitogen-activated protein kinase kinase kinase 8 (MAP3K8) mediates the LH-induced stimulation of progesterone synthesis in the porcine corpus luteum

2019 ◽  
Vol 31 (9) ◽  
pp. 1444
Author(s):  
Di Zhang ◽  
Ying Liu ◽  
Yan Cui ◽  
Sheng Cui
Keyword(s):  
Protein Kinase ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Kinase Inhibitor ◽  
Mitogen Activated Protein Kinase ◽  
Luteal Cells ◽  
Mitogen Activated Protein ◽  
Porcine Luteal Cells ◽  
Stimulation Of ◽  
Protein Kinase Kinase

Progesterone (P4) synthesized by the corpus luteum (CL) plays a key role in the establishment and maintenance of pregnancy. The LH signal is important for luteinisation and P4 synthesis in pigs. In a previous study, we demonstrated that mitogen-activated protein kinase kinase kinase 8 (MAP3K8) regulates P4 synthesis in mouse CL, but whether the function and mechanism of MAP3K8 in the pig is similar to that in the mouse is not known. Thus, in the present study we investigated the effects of MAP3K8 on porcine CL. Abundant expression of MAP3K8 was detected in porcine CL, and, in pigs, MAP3K8 expression was higher in mature CLs (or those of the mid-luteal phase) than in regressing CLs (late luteal phase). Further functional studies in cultured porcine luteal cells showed that P4 synthesis and the expression of genes encoding the key enzymes in P4 synthesis are significantly reduced when MAP3K8 is inhibited with the MAP3K8 inhibitor Tpl2 kinase inhibitor (MAP3K8i, 10μM). After 12–24h treatment of luteal cells with 100ngmL−1 LH, MAP3K8 expression and P4 secretion were significantly upregulated. In addition, the 10μM MAP3K8 inhibitor blocked the stimulatory effect of LH on P4 synthesis and extracellular signal-regulated kinase (ERK) 1/2 phosphorylation in porcine luteal cells. The LH-induced increases in MAP3K8 phosphorylation and expression, ERK1/2 phosphorylation and P4 synthesis were all blocked when protein kinase A was inhibited by its inhibitor H89 (20 μM) in porcine luteal cells. In conclusion, MAP3K8 mediates the LH-induced stimulation of P4 synthesis through the PKA/mitogen-activated protein kinase signalling pathway in porcine CL.

Immunohistochemical localization of pituitary gonadotrophins and gonadal steroids confirms the 'two-cell, two-gonadotrophin' hypothesis of steroidogenesis in the human ovary

1990 ◽  
Vol 126 (3) ◽  
pp. 483-NP ◽  
Author(s):  
M. Kobayashi ◽  
R. Nakano ◽  
A. Ooshima
Keyword(s):  
Luteal Phase ◽  
Immunohistochemical Analysis ◽  
Immunohistochemical Localization ◽  
Follicular Phase ◽  
Gonadal Steroids ◽  
Immunohistochemical Method ◽  
Human Ovary ◽  
Luteal Cells ◽  
Menstrual Cycles

ABSTRACT Ovaries from 37 women with normal menstrual cycles were analysed for localization of pituitary gonadotrophins and gonadal steroids using an immunohistochemical method. In the follicular phase, FSH and oestradiol-17β localized in the granulosa layer, and LH, progesterone and testosterone localized in the internal thecal layer. In the luteal phase, gonadotrophins and steroids localized in luteal cells. Particularly in the early luteal phase, FSH and oestradiol-17β localized in large luteal cells, and LH, progesterone and testosterone localized in small luteal cells. The results of the present immunohistochemical analysis confirm the two-cell, two-gonadotrophin hypothesis of steroidogenesis in the human ovary. Journal of Endocrinology (1990) 126, 483–488

scholarly journals The Effect of Uterine Flushings and Endometrial Protein Fractions on Progesterone Secretion by Porcine Luteal Cells

1986 ◽  
Vol 55 (3) ◽  
pp. 163-171
Author(s):  
Jadwiga Przala ◽  
Z. Luberda ◽  
A. Grazul ◽  
T. Wiesak ◽  
J. Kotwica
Keyword(s):  
Protein Fractions ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Porcine Luteal Cells

scholarly journals Immunohistological Localization and Expression of α-Actin in the Baboon (Papio anubis) Corpus Luteum

1997 ◽  
Vol 45 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Firyal S. Khan-Dawood ◽  
Jun Yang ◽  
M. Yusoff Dawood
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Adherens Junctions ◽  
Tunica Albuginea ◽  
Corpora Lutea ◽  
Papio Anubis ◽  
Lh Surge ◽  
Luteal Cells ◽  
Steroidogenic Cells ◽  
E Cadherin

We have recently shown the presence of E-cadherin and of α- and γ-catenins in human and baboon corpora lutea. These are components of adherens junctions between cells. The cytoplasmic catenins link the cell membrane-associated cadherins to the actin-based cytoskeleton. This interaction is necessary for the functional activity of the E-cad-herins. Our aim therefore was to determine the presence of α-actin in the baboon corpus luteum, to further establish whether the necessary components for E-cadherin activity are present in this tissue. An antibody specific for the smooth muscle isoform of actin, α-actin, was used for these studies. The results using immunohistochemistry show that (a) α-actin is present in steroidogenic cells of the active corpus luteum, theca externa of the corpus luteum, cells of the vasculature, and the tunica albuginea surrounding the ovary. The intensity of immunoreactivity for α-actin varied, with the cells of the vasculature reacting more intensely than the luteal cells. A difference in intensity of immunoreactivity was also observed among the luteal cells, with the inner granulosa cells showing stronger immunoreactivity than the peripheral theca lutein cells. There was no detectable immunoreactivity in the steroidogenic cells of the atretic corpus luteum. However, in both the active and atretic corpora lutea, α-actin-positive vascular cells were dispersed within the tissue. (b) Total α-actin (luteal and non-luteal), as determined by Western blot analyses, does not change during the luteal phase and subsequent corpus luteum demise (atretic corpora lutea). (c) hCG stimulated the expression of α-actin and progesterone secretion by the early luteal phase (LH surge + 1–5 days) and midluteal phase (LH surge + 6–10 days) cells in culture, but only progesterone in the late luteal phase (LH surge + 11–15 days). The data show that α-actin is present in luteal cells and that its expression is regulated by hCG, thus suggesting that E-cadherin may form functional adherens junctions in the corpus luteum.

Effects of Oxytocin on in Vitro Steroid Release of Midstage Small and Large Porcine Luteal Cells*

Endocrinology ◽  
1990 ◽  
Vol 126 (5) ◽  
pp. 2343-2349 ◽  
Author(s):  
LUTZ PITZEL ◽  
HUBERTUS JARRY ◽  
WOLFGANG WUTTKE
Keyword(s):  
Luteal Cells ◽  
Porcine Luteal Cells

Inhibitory Effect of Oxytocin and Vasopressin on Steroid Release by Cultured Porcine Luteal Cells*

Endocrinology ◽  
1988 ◽  
Vol 122 (5) ◽  
pp. 1780-1785 ◽  
Author(s):  
LUTZ PITZEL ◽  
IRMELIN PROBST ◽  
HUBERTUS JARRY ◽  
WOLFGANG WUTTKE
Keyword(s):  
Inhibitory Effect ◽  
Luteal Cells ◽  
Porcine Luteal Cells
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