Dilute acid pre-treatment of oilseed rape straw for bioethanol production

2011 ◽  
Vol 36 (9) ◽  
pp. 2424-2432 ◽  
Author(s):  
Anil Kuruvilla Mathew ◽  
Keith Chaney ◽  
Mitch Crook ◽  
Andrea Claire Humphries
2011 ◽  
Vol 102 (11) ◽  
pp. 6547-6553 ◽  
Author(s):  
Anil Kuruvilla Mathew ◽  
Keith Chaney ◽  
Mitch Crook ◽  
Andrea Claire Humphries

2017 ◽  
Vol 28 (3) ◽  
pp. 1 ◽  
Author(s):  
Charlie Marembu Dodo ◽  
Samphson Mamphweli ◽  
Omobola Okoh

Bioethanol production is one of the most promising possible substitutes for fossil-based fuels, but there is a need to make available cost-effective methods of production if it is to be successful. Various methods for the production of bioethanol using different feedstocks have been explored. Bioethanol synthesis from sugarcane, their tops and leaves have generally been regarded as waste and discarded. This investigation examined the use of lignocellulosic sugarcane leaves and tops as biomass and evaluated their hydrolysate content. The leaves and tops were hydrolysed using concentrated and dilute sulphuric acid and compared with a combination of oxidative alkali-peroxide pre-treatment with enzyme hydrolysis using the enzyme cellulysin® cellulase. Subsequent fermentation of the hydrolysates into bioethanol was done using the yeast saccharomyces cerevisae. The problem of acid hydrolysis to produce inhibitors was eliminated by overliming using calcium hydroxide and this treatment was subsequently compared with sodium hydroxide neutralisation. It was found that oxidative alkali pre-treatment with enzyme hydrolysis gave the highest yield of fermentable sugars of 38% (g/g) for 7% (v/v) peroxide pretreated biomass than 36% (g/g) for 5% (v/v) with the least inhibitors. Concentrated and dilute acid hydrolysis each gave yields of 25% (g/g) and 22% (g/g) respectively, although the acid required a neutralisation step, resulting in dilution. Alkaline neutralisation of acid hydrolysates using sodium hydroxide resulted in less dilution and loss of fermentable sugars, compared with overliming. Higher yields of bioethanol of 13.7 g/l were obtained from enzyme hydrolysates than the 6.9 g/l ethanol from dilute acid hydrolysates. There was more bioethanol yield of 13.7 g/l after 72 hours of fermentation with the yeast than the 7.0 g/l bioethanol after 24 hours.This research showed that it is possible to use sugarcane waste material to supplement biofuel requirements and that combining the chemical and biological methods of pretreatments can give higher yields at a faster rate.


2019 ◽  
Vol 33 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Benan İnan ◽  
Didem Özçimen

In the last decade, studies that have focused on biodiesel production from algal biomass have been replaced with bioethanol production from algae, because bioethanol production from algae seems more promising when assessed on economic terms. Most coastal areas are covered with macroalgae, which are considered as a waste, and thus become a great problem for the municipality. Instead of their disposal, they can be alternatively utilized for bioethanol production. In this study, macroalgae located in the coastal regions of the Marmara Sea were collected and utilized for bioethanol production, and effects of the concentration of pre-treatment chemicals, pre-treatment temperature, and pre-treatment time on bioethanol yield were investigated. The highest bioethanol yields for dilute acid and alkaline pre-treatments were obtained under the conditions of 2 N sulfuric acid and 0.15 N potassium hydroxide solutions at the pre-treatment temperature of 100 °C and pre-treatment time of 60 minutes.


2014 ◽  
Vol 154 ◽  
pp. 248-253 ◽  
Author(s):  
Anil Kuruvilla Mathew ◽  
Mitch Crook ◽  
Keith Chaney ◽  
Andrea Clare Humphries

2021 ◽  
Vol 13 (2) ◽  
pp. 643-655
Author(s):  
A. Thomas ◽  
M. Laxmi ◽  
A. Benny

With decades of studies on cellulose bioconversion, cellulases have been playing an important role in producing fermentable sugars from lignocellulosic biomass. Copious microorganisms that are able to degrade cellulose have been isolated and identified. The present study has been undertaken to isolate and screen the cellulase producing bacteria from soils of agrowaste field. Cellulase production has been qualitatively analyzed in carboxy methylcellulose (CMC) agar medium after congo red staining and NaCl treatment by interpretation with zones around the potent colonies. Out of the seven isolates, only two showed cellulase production. The morphogical and molecular characterization revealed its identity as Escherichia coli and Staphylococcus aureus. The potential of organisms for bioethanol production has been investigated using two substrates, namely, paper and leaves by subjecting with a pre-treatment process using acid hydrolysis to remove lignin which acts as physical barrier to cellulolytic enzymes. Ethanolic fermentation was done using Saccharomyces cerevisiae for 24-48 h and then the bioethanol produced was qualitatively proved by iodoform assay. These finding proves that ethanol can be made from the agricultural waste and the process is recommended as a means of generating wealth from waste.


Sign in / Sign up

Export Citation Format

Share Document