Molecular imprinting of doxorubicin by refolding thermally denatured bovine serum albumin and cross-linking with hydrogel network

2021 ◽  
Vol 168 ◽  
pp. 105036
Author(s):  
Muhua Zhao ◽  
Zezhou Li ◽  
Xinyi Li ◽  
Huaisyuan Xie ◽  
Qinyue Zhao ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Molecular Imprinting ◽  
Bovine Serum ◽  
Cross Linking ◽  
Hydrogel Network

Effect of some drugs and additives on the cross-linking of bovine serum albumin by glutaraldehyde

1989 ◽  
Vol 57 (3) ◽  
pp. 205-210 ◽  
Author(s):  
A.M. Saleh ◽  
L.K. El-Khordagui ◽  
D.A. Robb ◽  
Florence A.T.
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Linking ◽  
The Cross

New Photoactivators for Multiphoton Excited Three-dimensional Submicron Cross-linking of Proteins: Bovine Serum Albumin and Type 1 Collagen¶†

2007 ◽  
Vol 76 (2) ◽  
pp. 135-144
Author(s):  
Jonathan D. Pitts ◽  
Amy R. Howell ◽  
Rosa Taboada ◽  
Ipsita Banerjee ◽  
Jun Wang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Three Dimensional ◽  
Cross Linking

Combined cross-linking treatments of bovine serum albumin gel beadlets for controlled-delivery of caffeine

2009 ◽  
Vol 23 (5) ◽  
pp. 1398-1405 ◽  
Author(s):  
Chee-Yuen Gan ◽  
Lai-Hoong Cheng ◽  
Eng-Tong Phuah ◽  
Pei-Ni Chin ◽  
Abbas F.M. AlKarkhi ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Controlled Delivery ◽  
Cross Linking

Development and characterization of enzymatic biosensor based on lipase from porcine pancreas for propylparaben detection

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Leila Snani ◽  
Saida Zougar ◽  
Fatiha Benamia ◽  
Ilhem Ghodbane
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Pancreatic Lipase ◽  
Bovine Serum ◽  
Breast Tumors ◽  
Cross Linking ◽  
Porcine Pancreatic Lipase ◽  
Experimental Conditions ◽  
Content Type

Purpose The purpose of this paper is to study the immobilization of porcine pancreatic lipase (PPL), in an organic matrix by a covalent cross-linking method to sense propylparaben (PP) present in aqueous solution. Design/methodology/approach PPL immobilization was performed by the covalent cross-linking method, using bovine serum albumin (BSA) in the presence of saturated glutaraldehyde vapor (GA). The preparation of the enzymatic membrane involves the incorporation of porcine pancreatic lipase (PPL), bovine serum albumin (BSA) and glycerol into a phosphate buffer solution (PBS). Characterization of this sensor was performed by impedance spectroscopy (EIS) and scanning electron microscope (SEM). The effect of experimental conditions such as PPL activity, potential, scan rate, PP concentration, pH and presence of interfering elements were studied by cyclic voltammetry. Findings Under the optimal experimental conditions, a number of significant factors were optimized. The method exhibited good linearity in the range of 10–14 to 10–9 mol/L with a good correlation coefficient of 0.957, detection limit (LOD) of 3.66 × 10–15 mol/L and high sensitivity of 1.086 mA mol−1L. The authors also obtained a very good coverage rate of the surface equal to 91.44%, and hydrolytic activity of lipase is evaluated to 26.64 mmol min−1. The stability and the interference were also evaluated. The equivalent circuit used to explain the electrochemical behavior of modified electrode is a Randle circuit. Practical implications The main application of biosensors is the detection of biomolecules that are either indicators of a disease. For example, electrochemical biosensing techniques can be used as clinical tools to detect breast tumors, because these compounds (PP) were found in breast tumors. Originality/value The result registered in this paper indicates that the developed sensor is an efficient, fast, simple and inexpensive analytical tool that can be used for the analysis of water containing PP.

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