scholarly journals Zinc concentrations in serum and follicular fluid during ovarian stimulation and expression of Zn2+ transporters in human oocytes and cumulus cells

2011 ◽  
Vol 22 (6) ◽  
pp. 647-652 ◽  
Author(s):  
Yves Ménézo ◽  
Laurène Pluntz ◽  
Jacques Chouteau ◽  
Timur Gurgan ◽  
Aygul Demirol ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovarian Stimulation ◽  
Cumulus Cells ◽  
Human Oocytes ◽  
Zinc Concentrations

Association of serum and follicular fluid SHBG levels andSHBG(TAAAA)npolymorphism with follicle size in women undergoing ovarian stimulation

2010 ◽  
Vol 27 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Elissavet Hatzi ◽  
Ioanna Bouba ◽  
Anna Galidi ◽  
Leandros Lazaros ◽  
Nectaria Xita ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovarian Stimulation ◽  
Follicle Size

Human oocyte vitrification with corona radiata, in autologous follicular fluid supplemented with ethylene glycol, preserves conventional IVF potential: birth of four healthy babies

2014 ◽  
Vol 26 (7) ◽  
pp. 1001 ◽  
Author(s):  
Xian-Hong Tong ◽  
Li-Min Wu ◽  
Ren-Tao Jin ◽  
Hong-Bing Luan ◽  
Yu-Sheng Liu
Keyword(s):  
Ethylene Glycol ◽  
Embryo Transfer ◽  
Follicular Fluid ◽  
Control Group ◽  
Human Oocyte ◽  
Oocyte Vitrification ◽  
Human Oocytes ◽  
Corona Radiata ◽  
Fertilisation Rate ◽  
Significant Difference

The aim of this study was to examine the effects of vitrification with autologous follicular fluid (AFF) supplemented with ethylene glycol (EG) and sucrose on human oocytes with corona radiata. A total of 182 human oocytes with corona radiata from fifteen infertile patients were vitrified using either equilibration solutions (ES) and vitrification solution (VS) consisting of AFF, EG and sucrose (AFF group, n = 67) or commercial ES and VS (control group, n = 115). All oocytes were thawed in the next cycle, with surviving oocytes being inseminated by conventional IVF. The clinical outcome of vitrified–warmed oocytes by both vitrification methods was analysed retrospectively. In the AFF group, six patients received embryo transfer, with three couples taking four healthy babies home. In the control group, nine patients received embryo transfer, with four couples taking five healthy babies home. There was no significant difference in the survival rate (91.0 vs 92.2%), two pronuclei (2PN) fertilisation rate (73.8 vs 73.6%), cleavage rate (100 vs 100%), top-quality embryo rate (62.2 vs 59.2%), clinical pregnancy rate (50.0 vs 44.4%), implantation rate (33.3 vs 25%) or take-home baby rate (50.0 vs 44.4%) between the AFF group and the control group, respectively. These results show that AFF supplemented with EG and sucrose is an efficient, cost-effective cryoprotectant for human oocyte cryopreservation. A corona radiata on vitrified–warmed oocytes retains the oocytes’ fertilisation capability in conventional IVF.

Influence of Ovarian Stimulation on Follicular Fluid Steroid Levels and Fertilization of the Oocyte

1983 ◽  
pp. 73-82 ◽  
Author(s):  
J. Testart ◽  
R. Frydman ◽  
M. Castanier ◽  
B. Lassalle ◽  
J. Belaisch
Keyword(s):  
Follicular Fluid ◽  
Ovarian Stimulation

Induction of Mouse Peritoneum Mesenchymal Stem Cells into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media

2019 ◽  
Vol 28 (8) ◽  
pp. 554-564 ◽  
Author(s):  
Leila Mirzaeian ◽  
Poopak Eftekhari-Yazdi ◽  
Fereshteh Esfandiari ◽  
Farideh Eivazkhani ◽  
Mojtaba Rezazadeh Valojerdi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Follicular Fluid ◽  
Cumulus Cells ◽  
Conditioned Media

scholarly journals The Role of Interleukin-18 in Serum and Follicular Fluid during In Vitro Fertilization and Intracytoplasmic Sperm Injection

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Veronika Günther ◽  
Ibrahim Alkatout ◽  
Corinna Fuhs ◽  
Ali Salmassi ◽  
Liselotte Mettler ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Intracytoplasmic Sperm Injection ◽  
Ovarian Stimulation ◽  
The Body ◽  
Interleukin 18 ◽  
Vitro Fertilization ◽  
Median Level

Cytokines are key modulators of the immune system and play an important role in the ovarian cycle. IL-18 levels in serum and follicular fluid were analyzed in women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. The cohort study group consisted of 90 women, who were undergoing IVF or ICSI. The body mass index (BMI) was determined in all patients; IL-18 levels were measured in follicular fluid and serum. IL-18 levels in serum were significantly higher than those in follicular fluid. The median level in serum was 162.75 (80.21) pg/mL and that in follicular fluid, 138.24 (91.78) pg/mL. Women undergoing IVF treatment had lower IL-18 levels in serum (median, 151.19 (90.73) pg/mL) than those treated with ICSI (median, 163.57 (89.97) pg/mL). The correlation between IL-18 levels in serum and BMI was statistically significant, as well as the correlation between IL-18 levels in follicular fluid and ovarian stimulation response (p=0.003). IL-18 was correlated with the response to ovarian stimulation and was the reason for successful pregnancy after IVF or ICSI treatment. Among other cytokines, IL-18 appears to be a promising prognostic marker of success in reproductive treatment and should be evaluated as such in further prospective studies.

scholarly journals Predictive value of bovine follicular components as markers of oocyte developmental potential

2014 ◽  
Vol 26 (2) ◽  
pp. 337 ◽  
Author(s):  
Satoko Matoba ◽  
Katrin Bender ◽  
Alan G. Fahey ◽  
Solomon Mamo ◽  
Lorraine Brennan ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Cumulus Cell ◽  
Principal Component ◽  
Transcript Abundance ◽  
Cumulus Cells ◽  
Blastocyst Stage ◽  
Developmental Potential ◽  
Thecal Cells ◽  
Total Fatty Acids

The follicle is a unique micro-environment within which the oocyte can develop and mature to a fertilisable gamete. The aim of this study was to investigate the ability of a panel of follicular parameters, including intrafollicular steroid and metabolomic profiles and theca, granulosa and cumulus cell candidate gene mRNA abundance, to predict the potential of bovine oocytes to develop to the blastocyst stage in vitro. Individual follicles were dissected from abattoir ovaries, carefully ruptured under a stereomicroscope and the oocyte was recovered and individually processed through in vitro maturation, fertilisation and culture. The mean (± s.e.m.) follicular concentrations of testosterone (62.8 ± 4.8 ng mL–1), progesterone (616.8 ± 31.9 ng mL–1) and oestradiol (14.4 ± 2.4 ng mL–1) were not different (P > 0.05) between oocytes that formed (competent) or failed to form (incompetent) blastocysts. Principal-component analysis of the quantified aqueous metabolites in follicular fluid showed differences between oocytes that formed blastocysts and oocytes that degenerated; l-alanine, glycine and l-glutamate were positively correlated and urea was negatively correlated with blastocyst formation. Follicular fluid associated with competent oocytes was significantly lower in palmitic acid (P = 0.023) and total fatty acids (P = 0.031) and significantly higher in linolenic acid (P = 0.036) than follicular fluid from incompetent oocytes. Significantly higher (P < 0.05) transcript abundance of LHCGR in granulosa cells, ESR1 and VCAN in thecal cells and TNFAIP6 in cumulus cells was associated with competent compared with incompetent oocytes.

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