Induction of Mouse Peritoneum Mesenchymal Stem Cells into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media

2019 ◽  
Vol 28 (8) ◽  
pp. 554-564 ◽  
Author(s):  
Leila Mirzaeian ◽  
Poopak Eftekhari-Yazdi ◽  
Fereshteh Esfandiari ◽  
Farideh Eivazkhani ◽  
Mojtaba Rezazadeh Valojerdi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Follicular Fluid ◽  
Cumulus Cells ◽  
Conditioned Media

scholarly journals Wharton's jelly derived mesenchymal stem cells differentiate into oocyte like cells in vitro by follicular fluid and cumulus cells conditioned medium

Heliyon ◽  
2020 ◽  
Vol 6 (10) ◽  
pp. e04992
Author(s):  
Mona Zolfaghar ◽  
Leila Mirzaeian ◽  
Bahareh Beiki ◽  
Tahereh Naji ◽  
Ashraf Moini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Follicular Fluid ◽  
Cumulus Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

scholarly journals Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells

2021 ◽  
Author(s):  
Cristina Russo ◽  
Giuliana Mannino ◽  
Martina Patanè ◽  
Nunziatina Laura Parrinello ◽  
Rosalia Pellitteri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Neural Differentiation ◽  
Conditioned Media ◽  
Glial Fibrillary Acid Protein ◽  
Ghrelin Receptor ◽  
Neuronal Markers ◽  
Adipose Mesenchymal Stem Cells ◽  
Marker Expression

AbstractThe influences of ghrelin on neural differentiation of adipose-derived mesenchymal stem cells (ASCs) were investigated in this study. The expression of typical neuronal markers, such as protein gene product 9.5 (PGP9.5) and Microtubule Associated Protein 2 (MAP2), as well as glial Fibrillary Acid Protein (GFAP) as a glial marker was evaluated in ASCs in different conditions. In particular, 2 µM ghrelin was added to control ASCs and to ASCs undergoing neural differentiation. For this purpose, ASCs were cultured in Conditioned Media obtained from Olfactory Ensheathing cells (OEC-CM) or from Schwann cells (SC-CM). Data on marker expression were gathered after 1 and 7 days of culture by fluorescence immunocytochemistry and flow cytometry. Results show that only weak effects were induced by the addition of only ghrelin. Instead, dynamic ghrelin-induced modifications were detected on the increased marker expression elicited by glial conditioned media. In fact, the combination of ghrelin and conditioned media consistently induced a further increase of PGP9.5 and MAP2 expression, especially after 7 days of treatment. The combination of ghrelin with SC-CM produced the most evident effects. Weak or no modifications were found on conditioned medium-induced GFAP increases. Observations on the ghrelin receptor indicate that its expression in control ASCs, virtually unchanged by the addition of only ghrelin, was considerably increased by CM treatment. These increases were enhanced by combining ghrelin and CM treatment, especially at 7 days. Overall, it can be assumed that ghrelin favors a neuronal rather than a glial ASC differentiation.

scholarly journals Electrospun fibers enhanced the paracrine signaling of mesenchymal stem cells for cartilage regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nurul Dinah Kadir ◽  
Zheng Yang ◽  
Afizah Hassan ◽  
Vinitha Denslin ◽  
Eng Hin Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrospun Fiber ◽  
Cartilage Regeneration ◽  
Conditioned Media ◽  
Biologically Active ◽  
Paracrine Signaling ◽  
Therapeutic Value ◽  
A Cell ◽  
Fiber Sheet

Abstract Background Secretome profiles of mesenchymal stem cells (MSCs) are reflective of their local microenvironments. These biologically active factors exert an impact on the surrounding cells, eliciting regenerative responses that create an opportunity for exploiting MSCs towards a cell-free therapy for cartilage regeneration. The conventional method of culturing MSCs on a tissue culture plate (TCP) does not provide the physiological microenvironment for optimum secretome production. In this study, we explored the potential of electrospun fiber sheets with specific orientation in influencing the MSC secretome production and its therapeutic value in repairing cartilage. Methods Conditioned media (CM) were generated from MSCs cultured either on TCP or electrospun fiber sheets of distinct aligned or random fiber orientation. The paracrine potential of CM in affecting chondrogenic differentiation, migration, proliferation, inflammatory modulation, and survival of MSCs and chondrocytes was assessed. The involvement of FAK and ERK mechanotransduction pathways in modulating MSC secretome were also investigated. Results We showed that conditioned media of MSCs cultured on electrospun fiber sheets compared to that generated from TCP have improved secretome yield and profile, which enhanced the migration and proliferation of MSCs and chondrocytes, promoted MSC chondrogenesis, mitigated inflammation in both MSCs and chondrocytes, as well as protected chondrocytes from apoptosis. Amongst the fiber sheet-generated CM, aligned fiber-generated CM (ACM) was better at promoting cell proliferation and augmenting MSC chondrogenesis, while randomly oriented fiber-generated CM (RCM) was more efficient in mitigating the inflammation assault. FAK and ERK signalings were shown to participate in the modulation of MSC morphology and its secretome production. Conclusions This study demonstrates topographical-dependent MSC paracrine activities and the potential of employing electrospun fiber sheets to improve the MSC secretome for cartilage regeneration.

Comparative immunomodulatory properties of adipose-derived mesenchymal stem cells conditioned media from BALB/c, C57BL/6, and DBA mouse strains

2013 ◽  
Vol 114 (4) ◽  
pp. 955-965 ◽  
Author(s):  
Seyed Mahmoud Hashemi ◽  
Zuhair Mohammad Hassan ◽  
Ali Akbar Pourfathollah ◽  
Sara Soudi ◽  
Abbas Shafiee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Media ◽  
Mouse Strains ◽  
Immunomodulatory Properties

Organic and inorganic zinc show similar regulatory effects on the expression of some germ cell specific markers induced in bone marrow mesenchymal stem cells after treatment with retinoic acid

Biologia ◽  
2019 ◽  
Vol 74 (12) ◽  
pp. 1721-1731
Author(s):  
Maryam Ghasemzadeh-Hasankolaei ◽  
Forough Azam Sayahpour ◽  
Mohammad Ghasemzadeh-Hasankolaei ◽  
Mohammad Taghi Ghorbanian ◽  
Mohamadreza B. Eslaminejad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Marrow Mesenchymal Stem Cells ◽  
Regulatory Effects
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