The expression level of miR-203 in patients with gastric cancer and its clinical significance

2017 ◽  
Vol 213 (12) ◽  
pp. 1515-1518 ◽  
Author(s):  
Yushuang Zheng ◽  
Wei Liu ◽  
Lingchuan Guo ◽  
Xianghong Yang
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Expression Level

scholarly journals Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Wuping Yang ◽  
Kenan Zhang ◽  
Lei Li ◽  
Yawei Xu ◽  
Kaifang Ma ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Renal Cell Carcinoma ◽  
Protein Expression ◽  
Cell Carcinoma ◽  
Clinical Significance ◽  
Renal Cell ◽  
Clear Cell ◽  
Expression Level ◽  
Cell Renal Cell Carcinoma ◽  
Qrt Pcr

Abstract Background Emerging evidence confirms that lncRNAs (long non-coding RNAs) are potential biomarkers that play vital roles in tumors. ZNF582-AS1 is a novel lncRNA that serves as a potential prognostic marker of cancers. However, the specific clinical significance and molecular mechanism of ZNF582-AS1 in ccRCC (clear cell renal cell carcinoma) are unclear. Methods Expression level and clinical significance of ZNF582-AS1 were determined by TCGA-KIRC data and qRT-PCR results of 62 ccRCCs. DNA methylation status of ZNF582-AS1 promoter was examined by MSP, MassARRAY methylation and demethylation analysis. Gain-of-function experiments were conducted to investigate the biological roles of ZNF582-AS1 in the phenotype of ccRCC. The subcellular localization of ZNF582-AS1 was detected by RNA FISH. iTRAQ, RNA pull-down and RIP-qRT-PCR were used to identify the downstream targets of ZNF582-AS1. rRNA MeRIP-seq and MeRIP-qRT-PCR were utilized to examine the N(6)-methyladenosine modification status. Western blot and immunohistochemistry assays were used to determine the protein expression level. Results ZNF582-AS1 was downregulated in ccRCC, and decreased ZNF582-AS1 expression was significantly correlated with advanced tumor stage, higher pathological stage, distant metastasis and poor prognosis. Decreased ZNF582-AS1 expression was caused by DNA methylation at the CpG islands within its promoter. ZNF582-AS1 overexpression inhibited cell proliferative, migratory and invasive ability, and increased cell apoptotic rate in vitro and in vivo. Mechanistically, we found that ZNF582-AS1 overexpression suppressed the N(6)-methyladenosine modification of MT-RNR1 by reducing rRNA adenine N(6)-methyltransferase A8K0B9 protein level, resulting in the decrease of MT-RNR1 expression, followed by the inhibition of MT-CO2 protein expression. Furthermore, MT-RNR1 overexpression reversed the decreased MT-CO2 expression and phenotype inhibition of ccRCC induced by increased ZNF582-AS1 expression. Conclusions This study demonstrates for the first time that ZNF582-AS1 functions as a tumor suppressor gene in ccRCC and ZNF582-AS1 may serve as a potential biomarker and therapeutic target of ccRCC.

scholarly journals miR-21 Is a Promising Novel Biomarker for Lymph Node Metastasis in Patients with Gastric Cancer

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Yuejuan Xu ◽  
Jue Sun ◽  
Jianhua Xu ◽  
Qi Li ◽  
Yuewu Guo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Malignant Disease ◽  
Close Association ◽  
Survival Rates ◽  
Expression Level ◽  
Human Gastric Cancer ◽  
Node Metastasis ◽  
Kaplan Meier

Background. Gastric cancer (GC) is an important malignant disease around the world. Abnormalities of microRNAs (miRNAs) have been implicated in carcinogenesis of various cancers. In the present study, we examined miR-21 expression in human gastric cancer with lymph node metastasis and attempted to uncover its relationship with clinicopathologic data, especially with lymph node metastasis.Materials and Methods. The expression levels of miR-21 in the tumor specimens of GC patients were quantified by RT-PCR. The correlation between miR-21 level and multiple clinicopathological factors was then examined by Mann-Whitney test, Kaplan-Meier survival analysis, and operating characteristic (ROC) analysis.Results. The expression level of miR-21 was higher in GC patients with lymph node metastasis than in those without lymph node metastasis (P<0.05). Expression level of miR-21 was significantly correlated with histologic type, T stage, lymph node metastasis and pTNM stage. The overall survival rates in GC patients with low upregulated miR-21 expression were significantly higher than those with high upregulated miR-21 (P<0.05).Conclusion. A close association is implicated between the elevated miR-21and lymph node metastasis, which could potentially be exploited as a practical biomarker for lymph node metastasis in patients with GC.

scholarly journals Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages

2016 ◽  
Vol 11 (3) ◽  
pp. 2271-2277 ◽  
Author(s):  
BINGYUAN FEI ◽  
FUJIAN JI ◽  
XUEBO CHEN ◽  
ZHUO LIU ◽  
SHUO LI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Beclin 1 ◽  
Clinical Stages ◽  
Cancer Tissues

Clinical significance of RacGAP1 expression at the invasive front of gastric cancer

2014 ◽  
Vol 18 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Susumu Saigusa ◽  
Koji Tanaka ◽  
Yasuhiko Mohri ◽  
Masaki Ohi ◽  
Tadanobu Shimura ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Invasive Front

scholarly journals Clinical Significance of MiR-137 Expression in Patients with Gastric Cancer After Radical Gastrectomy

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142377 ◽  
Author(s):  
Qiaoyan Gu ◽  
Jun Zhang ◽  
Haifeng Hu ◽  
Yu-e Tan ◽  
Shengmei Shi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Radical Gastrectomy

scholarly journals Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer

2018 ◽  
Vol Volume 10 ◽  
pp. 1479-1486 ◽  
Author(s):  
Juan-Yi Wang ◽  
Tao Wu ◽  
Wu Ma ◽  
Shuo Li ◽  
Wen-Jiang Jing ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Significance ◽  
Emt Markers
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