The comparison of methods to identify the presence of fibrocytes in formalin-fixed, paraffin-embedded archival cardiac tissue with coronary heart disease

2014 ◽  
Vol 210 (12) ◽  
pp. 929-933
Author(s):  
Pu-Ping Lei ◽  
Qun Shuai ◽  
Shang-Wen Wang ◽  
Si-Ming Tao ◽  
Yong-Qiang Qu ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Cardiac Tissue ◽  
Comparison Of Methods ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

2010 ◽  
Vol 400 (1) ◽  
pp. 110-117 ◽  
Author(s):  
John B.A. Okello ◽  
Jaymi Zurek ◽  
Alison M. Devault ◽  
Melanie Kuch ◽  
Andrew L. Okwi ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Comparison Of Methods ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Autopsy Tissues ◽  
Formalin Fixed

scholarly journals Chronic Occupational Exposure to Ionizing Radiation Induces Alterations in the Structure and Metabolism of the Heart: A Proteomic Analysis of Human Formalin-Fixed Paraffin-Embedded (FFPE) Cardiac Tissue

2020 ◽  
Vol 21 (18) ◽  
pp. 6832
Author(s):  
Omid Azimzadeh ◽  
Tamara Azizova ◽  
Juliane Merl-Pham ◽  
Andreas Blutke ◽  
Maria Moseeva ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Gamma Ray ◽  
Confounding Factor ◽  
Control Group ◽  
Histopathological Analysis ◽  
Label Free ◽  
Proteomics Analysis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Epidemiological studies on workers employed at the Mayak plutonium enrichment plant have demonstrated an association between external gamma ray exposure and an elevated risk of ischemic heart disease (IHD). In a previous study using fresh-frozen post mortem samples of the cardiac left ventricle of Mayak workers and non-irradiated controls, we observed radiation-induced alterations in the heart proteome, mainly downregulation of mitochondrial and structural proteins. As the control group available at that time was younger than the irradiated group, we could not exclude age as a confounding factor. To address this issue, we have now expanded our study to investigate additional samples using archival formalin-fixed paraffin-embedded (FFPE) tissue. Importantly, the control group studied here is older than the occupationally exposed (>500 mGy) group. Label-free quantitative proteomics analysis showed that proteins involved in the lipid metabolism, sirtuin signaling, mitochondrial function, cytoskeletal organization, and antioxidant defense were the most affected. A histopathological analysis elucidated large foci of fibrotic tissue, myocardial lipomatosis and lymphocytic infiltrations in the irradiated samples. These data highlight the suitability of FFPE material for proteomics analysis. The study confirms the previous results emphasizing the role of adverse metabolic changes in the radiation-associated IHD. Most importantly, it excludes age at the time of death as a confounding factor.

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